BAIAP2 Antibody (OTI 1D9)

Novus Biologicals | Catalog # NBP2-00810

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI 1D9
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Product Specifications

Immunogen

Full length human recombinant protein of human BAIAP2 (NP_006331) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

57.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for BAIAP2 Antibody (OTI 1D9)

Western Blot: BAIAP2 Antibody (OTI 1D9) [NBP2-00810]

Western Blot: BAIAP2 Antibody (OTI 1D9) [NBP2-00810]

Western Blot: BAIAP2 Antibody (1D9) [NBP2-00810] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BAIAP2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BAIAP2.
Immunocytochemistry/Immunofluorescence: BAIAP2 Antibody (1D9) [NBP2-00810] Staining of COS7 cells transiently transfected by pCMV6-ENTRY BAIAP2.
Immunohistochemistry-Paraffin: BAIAP2 Antibody (OTI 1D9) [NBP2-00810]

Immunohistochemistry-Paraffin: BAIAP2 Antibody (OTI 1D9) [NBP2-00810]

Immunohistochemistry-Paraffin: BAIAP2 Antibody (1D9) [NBP2-00810] - Staining of paraffin-embedded Human prostate tissue using anti-BAIAP2 mouse monoclonal antibody.
Flow Cytometry: BAIAP2 Antibody (OTI 1D9) [NBP2-00810]

Flow Cytometry: BAIAP2 Antibody (OTI 1D9) [NBP2-00810]

Flow Cytometry: BAIAP2 Antibody (1D9) [NBP2-00810] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-BAIAP2 antibody, and then analyzed by flow cytometry.
Immunohistochemistry-Paraffin: BAIAP2 Antibody (OTI 1D9) [NBP2-00810]

Immunohistochemistry-Paraffin: BAIAP2 Antibody (OTI 1D9) [NBP2-00810]

Immunohistochemistry-Paraffin: BAIAP2 Antibody (1D9) [NBP2-00810] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-BAIAP2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: BAIAP2 Antibody (OTI 1D9) [NBP2-00810]

Immunohistochemistry-Paraffin: BAIAP2 Antibody (OTI 1D9) [NBP2-00810]

Immunohistochemistry-Paraffin: BAIAP2 Antibody (1D9) [NBP2-00810] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-BAIAP2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: BAIAP2 Antibody (OTI 1D9) [NBP2-00810]

Immunohistochemistry-Paraffin: BAIAP2 Antibody (OTI 1D9) [NBP2-00810]

Immunohistochemistry-Paraffin: BAIAP2 Antibody (1D9) [NBP2-00810] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-BAIAP2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: BAIAP2 Antibody (OTI 1D9) [NBP2-00810]

Immunohistochemistry-Paraffin: BAIAP2 Antibody (OTI 1D9) [NBP2-00810]

Immunohistochemistry-Paraffin: BAIAP2 Antibody (1D9) [NBP2-00810] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-BAIAP2 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: BAIAP2 Antibody (OTI 1D9) [NBP2-00810]

Immunohistochemistry-Paraffin: BAIAP2 Antibody (OTI 1D9) [NBP2-00810]

Immunohistochemistry-Paraffin: BAIAP2 Antibody (1D9) [NBP2-00810] - Staining of paraffin-embedded Human Kidney tissue using anti-BAIAP2 mouse monoclonal antibody.

Applications for BAIAP2 Antibody (OTI 1D9)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.65 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: BAIAP2

The protein encoded by this gene has been identified as a brain-specific angiogenesis inhibitor (BAI1)-binding protein. This adaptor protein links membrane bound G-proteins to cytoplasmic effector proteins. This protein functions as an insulin receptor tyrosine kinase substrate and suggests a role for insulin in the central nervous system. It also associates with a downstream effector of Rho small G proteins, which is associated with the formation of stress fibers and cytokinesis. This protein is involved in lamellipodia and filopodia formation in motile cells and may affect neuronal growth-cone guidance. This protein has also been identified as interacting with the dentatorubral-pallidoluysian atrophy gene, which is associated with an autosomal dominant neurodegenerative disease. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Alternate Names

BAI1-associated protein 2IRS-58, BAI-associated protein 2, BAP2, brain-specific angiogenesis inhibitor 1-associated protein 2, Fas ligand-associated factor 3, FLAF3, Insulin receptor substrate p53, Insulin receptor substrate p53/p58, Insulin receptor substrate protein of 53 kDa, IRSP53, IRSp53/58, Protein BAP2

Entrez Gene IDs

10458 (Human)

Gene Symbol

BAIAP2

Additional BAIAP2 Products

Product Documents for BAIAP2 Antibody (OTI 1D9)

Certificate of Analysis

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Product Specific Notices for BAIAP2 Antibody (OTI 1D9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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