BANP Antibody - BSA Free

Novus Biologicals | Catalog # NB100-79784

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all BANP Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 425 and the C-terminus (residue 469) of human BTG3 associated nuclear protein using the numbering given in TrEMBL entry Q96GJ7 (GeneID 54971).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BANP Antibody - BSA Free

Western Blot: BANP Antibody [NB100-79784]

Western Blot: BANP Antibody [NB100-79784]

Western Blot: BANP Antibody [NB100-79784] - Detection of human and mouse SMAR1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMAR1 antibody NB100-79784 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry: BANP Antibody [NB100-79784]

Immunohistochemistry: BANP Antibody [NB100-79784]

Immunohistochemistry: BANP Antibody [NB100-79784] - Detection of human SMAR1 by immunohistochemistry. Samples: FFPE sections of human lung carcinoma. Antibodies: Affinity purified rabbit anti-SMAR1 Cat. No. NB100-79784 Lot 2 used at a dilution of 1:5,000 (0.2ug/ml) (left) and Cat. No. NB100-79784 Lot3 used at a dilution of 1:5,000 (0.2ug/ml) (right). Detection: DAB
Western Blot: BANP Antibody [NB100-79784]

Western Blot: BANP Antibody [NB100-79784]

Western Blot: BANP Antibody [NB100-79784] - Nuclear extract (NE) from HeLa cells. Antibody used at 0.1 ug/ml.
Western Blot: BANP Antibody [NB100-79784]

Western Blot: BANP Antibody [NB100-79784]

Western Blot: BANP Antibody [NB100-79784] - Detection of Human, Mouse and Rat SMAR1/BANP by Western Blot. Samples: Whole cell lysate (50 ug) from mouse 4T1, mouse CT26.WT, rat C6, HeLa, and 293T cells. Antibodies: Affinity purified rabbit anti-SMAR1/BANP antibody NB100-79784 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunohistochemistry-Paraffin: BANP Antibody [NB100-79784]

Immunohistochemistry-Paraffin: BANP Antibody [NB100-79784]

Immunohistochemistry-Paraffin: BANP Antibody [NB100-79784] - FFPE section of human breast tumor. Affinity purified rabbit anti-SMAR1/BANP used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit
Immunohistochemistry: BANP Antibody [NB100-79784]

Immunohistochemistry: BANP Antibody [NB100-79784]

Immunohistochemistry: BANP Antibody [NB100-79784] - Sample: FFPE sections of human lung carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti-fs18 SMAR1 fs20 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB
Immunohistochemistry: BANP Antibody [NB100-79784]

Immunohistochemistry: BANP Antibody [NB100-79784]

Immunohistochemistry: BANP Antibody [NB100-79784] - Detection of mouse SMAR1 by immunohistochemistry. Samples: FFPE sections of mouse renal cell carcinoma. Antibodies: Affinity purified rabbit anti-SMAR1 Cat. No. NB100-79784 Lot 2 used at a dilution of 1:5,000 (0.2ug/ml) (left) and Cat. No. NB100-79784 Lot3 used at a dilution of 1:5,000 (0.2ug/ml) (right). Detection: DAB
Immunoprecipitation: BANP Antibody [NB100-79784]

Immunoprecipitation: BANP Antibody [NB100-79784]

Immunoprecipitation: BANP Antibody [NB100-79784] - Detection of human SMAR1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SMAR1 antibody NB100-79784 (lot NB100-79784-3) used for IP at 6 ug per reaction. SMAR1 was also immunoprecipitated by a previous lot of this antibody (lot NB100-79784-2). For blotting immunoprecipitated SMAR1, NB100-79784 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for BANP Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:500-1:2500

Western Blot

1:10000-1:25000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissues.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: BANP

This gene encodes a protein that binds to matrix attachment regions. The protein functions as a tumor suppressor and cell cycle regulator. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Alternate Names

BEN domain containing 1, BEND1BEN domain-containing protein 1, BTG3 associated nuclear protein, Btg3-associated nuclear protein, DKFZp761H172, FLJ10177, FLJ20538, protein BANP, SMAR1Scaffold/matrix-associated region-1-binding protein, SMARBP1

Entrez Gene IDs

54971 (Human)

Gene Symbol

BANP

UniProt

Q96GJ7 (Human)

Additional BANP Products

Product Documents for BANP Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for BANP Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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