Bassoon Antibody (HL1732) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-25376

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1732 expressed in HEK293

Format

Azide and BSA Free
View all Bassoon Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide of mouse Bassoon

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Bassoon Antibody (HL1732) - Azide and BSA Free

Bassoon Antibody (HL1732) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Bassoon Antibody (HL1732) [NBP3-25376] -

Bassoon antibody [HL1732] detects Bassoon protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neurons cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Bassoon stained by Bassoon antibody [HL1732] (NBP3-25376) diluted at 1:250.
Red: Tau, a axon marker, stained by Tau antibody [GT287] diluted at 1:500.
Blue: Fluoroshield with DAPI.
Bassoon Antibody (HL1732) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Bassoon Antibody (HL1732) [NBP3-25376] -

Bassoon antibody [HL1732] detects Bassoon protein at synapse by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Bassoon stained by Bassoon antibody [HL1732] (NBP3-25376) diluted at 1:250.
Blue: Fluoroshield with DAPI.

Applications for Bassoon Antibody (HL1732) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Bassoon

Bassoon is a 420 kDa protein that is a localized at the presynaptic nerve terminals and is believed to play a role in the structural and functional organization of the synaptic vesicle cycle (1-3). Bassoon does not belong to any known protein families. It has been found in rat and mouse with sequence identity of the two proteins at 96% (1). The human BASSOON gene has recently been cloned and localized (3). Bassoon is predicted to contain two double-zinc fingers, three coiled-coil regions, and two polyglutamine domains (1). The polyglutamine domains in the C-terminus are of interest, since it is known that for some human proteins, such as Huntingtin, abnormal amplification of this region can cause late-onset neurodegeneration (1,3). Bassoon is concentrated at sites opposite to postsynaptic densities in synaptic terminals and in cultured neurons, it is found to colocalize with GABA (A) and glutamate (GluR1) receptors (2). Another presynaptic protein, Piccolo (4) was found to colocalize with Bassoon in cultured hippocampal neurons (1). These observations suggested that they serve specific functions at synaptic junctions and may be involved in organization of the cytoskeleton at the site of neurotransmitter release (1).

Alternate Names

bassoon (presynaptic cytomatrix protein)

Gene Symbol

BSN

Additional Bassoon Products

Product Documents for Bassoon Antibody (HL1732) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Bassoon Antibody (HL1732) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Bassoon Antibody (HL1732) - Azide and BSA Free

There are currently no reviews for this product. Be the first to review Bassoon Antibody (HL1732) - Azide and BSA Free and earn rewards!

Have you used Bassoon Antibody (HL1732) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Bassoon Antibody (HL1732) - Azide and BSA Free

Showing  1 - 1 of 1 FAQ Showing All

  • Q: I am looking for a good antibody to detect Bassoon in mouse brain slices (PFA fixed); can you recommend any one for this?

    A:

    After searching through our product catalog list, we have found the following antibodies which may be of interest: Bassoo. Using the filters on the left column of the site should allow you to filter by species and desired application. Any antibody that is listed to show validation for use in IHC-P and has also been validated in mouse samples will be backed by our 100% Guarantee. Under the terms and conditions of the guarantee, Novus will provide you with a full refund or replacement should the antibody fail to perform, as specified, in samples expressing the protein.

Showing  1 - 1 of 1 FAQ Showing All
View all FAQs for Antibodies