Bax Antibody (HL236) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-13676

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL236 expressed in HEK293

Format

Azide and BSA Free
View all Bax Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Bax. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for Bax Antibody (HL236) - Azide and BSA Free

Western Blot: Bax Antibody (HL236) [NBP3-13676]

Western Blot: Bax Antibody (HL236) [NBP3-13676]

Western Blot: Bax Antibody (HL236) [NBP3-13676] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Bax antibody [HL236] (NBP3-13676) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Immunohistochemistry-Paraffin: Bax Antibody (HL236) [NBP3-13676]

Immunohistochemistry-Paraffin: Bax Antibody (HL236) [NBP3-13676]

Immunohistochemistry-Paraffin: Bax Antibody (HL236) [NBP3-13676] - Bax antibody [HL236] detects Bax protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. Bax stained by Bax antibody [HL236] (NBP3-13676) diluted at 1:50. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: Bax Antibody (HL236) [NBP3-13676]

Western Blot: Bax Antibody (HL236) [NBP3-13676]

Western Blot: Bax Antibody (HL236) [NBP3-13676] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Bax antibody [HL236] (NBP3-13676) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Bax Antibody (HL236)

Western Blot: Bax Antibody (HL236) [NBP3-13676] -

Western Blot: Bax Antibody (HL236) [NBP3-13676] - Raw264.7 whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Bax antibody [HL236] (NBP3-13676) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Bax Antibody (HL236)

Western Blot: Bax Antibody (HL236) [NBP3-13676] -

Western Blot: Bax Antibody (HL236) [NBP3-13676] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Bax antibody [HL236] (NBP3-13676) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Bax Antibody (HL236)

Immunohistochemistry-Paraffin: Bax Antibody (HL236) [NBP3-13676] -

Immunohistochemistry-Paraffin: Bax Antibody (HL236) [NBP3-13676] - Bax antibody [HL236] detects Bax protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
Bax stained by Bax antibody [HL236] (NBP3-13676) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Bax Antibody (HL236)

Immunohistochemistry-Paraffin: Bax Antibody (HL236) [NBP3-13676] -

Immunohistochemistry-Paraffin: Bax Antibody (HL236) [NBP3-13676] - Bax antibody [HL236] detects Bax protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
Bax stained by Bax antibody [HL236] (NBP3-13676) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Bax Antibody (HL236)

Western Blot: Bax Antibody (HL236) [NBP3-13676] -

Western Blot: Bax Antibody (HL236) [NBP3-13676] - Wild-type (WT) and Bax knockout (KO) Expi293 cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Bax antibody [HL236] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Bax Antibody (HL236)

Western Blot: Bax Antibody (HL236) [NBP3-13676] -

Western Blot: Bax Antibody (HL236) [NBP3-13676] - Wild-type (WT) and NME4 knockout (KO) HeLa cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Bax antibody [HL236] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for Bax Antibody (HL236) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Bax

The Bcl-2 family of apoptosis-related genes plays central roles in regulating apoptotic pathways (reviewed in Thomadaki and Scorilas, 2006). Regulation of cell death through apoptosis is critical for the maintenance of homeostasis, defense against infectious agents, and normal development. Bcl-2 family proteins regulate apoptosis primarily through the regulation of mitochondrial outer membrane permeability. In mammals, the family consists of both prosurvival (antiapoptotic) and proapoptotic (prodeath) members. Cellular homeostasis is thought to be dependent on a balance between the actions of prosurvival and proapoptotic proteins. Bcl-2 family proteins can be divided into 3 main subfamilies on the basis of their function and the content of their Bcl-2 homology (BH) domains, for example: 1) Prosurvival: Bcl-2, Bcl-XL, Bcl-W, A1, and Mcl-1 2) Proapoptotic (multidomain): Bax, Bak, and Bok. 3) BH3-only (proapoptotic): Bad, Bcl-XS, Bid, Bik, Bim, Blk, Bmf, Bnip, Noxa, and Puma. Prosurvival members inhibit cells from undergoing apoptosis, whereas proapoptotic and BH3-only subfamily members promote apoptosis. There are 4 BH domains (1-4) conserved among Bcl-2 family proteins. The BH domains are important for function as well as for heterodimerization between family members. Typical prosurvival family members have all four BH domains (1-4), whereas proapoptotic (multidomain) members have BH1, 2 and 3 domains and BH3-only members have only the BH3 domain. Overall, the relative ratio of prosurvival and proapoptotic proteins determines the suseptibility of a cell to various apoptotic stimuli. Many Bcl-2 family proteins are differentially expressed in various malignancies and some are useful prognostic biomarkers. Prosurvival proteins are often elevated in diverse cancers and have the potential to confer resistance to both endogenous cell death stimuli and cancer treatments. Alterations in the ratio or levels of Bcl-2 family proteins have been also associated with nonmalignant diseases including neurodegenerative diseases, autoimmune diseases, AIDs, Down's syndrome, cardiovascular diseases, diabetes, glomerulonephritis, and muscular dystrophy. IMG-5682 recognizes Bax. It reacts with an epitope (PELALDPVPQDASTKKLSE)which is 100% conserved in human Bax isoforms alpha (192 amino acids), beta (218 amino acids), epsilon (164 amino acids), sigma (179 amino acids), and psi (173 amino acids).

Long Name

Bcl Associated X Protein

Alternate Names

apoptosis regulator BAX, BCL2-associated X protein, Bcl2-L-4, BCL2L4bcl2-L-4, Bcl-2-like protein 4

Gene Symbol

BAX

Additional Bax Products

Product Documents for Bax Antibody (HL236) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Bax Antibody (HL236) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Bcl-2 Proteins and Regulators

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Protocols

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FAQs

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