Bax inhibitor 1 Antibody (20F565)
Novus Biologicals | Catalog # NBP2-24912
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # 20F565
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Product Specifications
Immunogen
A synthetic peptide made to the N-terminal portion of the human Bax inhibitor 1 protein (between amino acids 1-40) UniProt P55061.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Scientific Data Images for Bax inhibitor 1 Antibody (20F565)
Western Blot: Bax inhibitor 1 Antibody (20F565) [NBP2-24912]
Western Blot: Bax inhibitor 1 Antibody (20F565) [NBP2-24912] - analysis of Bi-1 with this antibody. Lane 1: in vitro translated human Bi-1 protein. Lanes 2 and 3: IP/WB of Bi-1 protein enriched in the transgenic mouse brain (Lane 2) or wildtype brain (Lane 3). IP was done with a polyclonal Bi-1 antibody, followed by WB with this antibody.Immunohistochemistry-Paraffin: Bax inhibitor 1 Antibody (20F565) [NBP2-24912]
Immunohistochemistry-Paraffin: Bax inhibitor 1 Antibody (20F565) [NBP2-24912] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human liver cancer using 5 ug/ml concentration of Bax inhibitor 1 antibody (clone 20F565). Cytoplasmic immuno-positivity of Bax inhibitor 1 (TMBIM6) was observed in the hepatis cancer cells whereas the cells of tumor stroma did not develop any staining [10X Magnification]Immunohistochemistry-Paraffin: Bax inhibitor 1 Antibody (20F565) [NBP2-24912]
Immunohistochemistry-Paraffin: Bax inhibitor 1 Antibody (20F565) [NBP2-24912] - analysis of Bi-1 using Bi-1 antibody at 5 ug/ml: A) overview of stained human and mouse TMA, B-C) immunoreactivity in human archicortex (positive neurons of hippocampal gyrus) at 200X and 400X, D-E) strong immunoreactivity in neurons of human dorsal root ganglion.Immunohistochemistry-Paraffin: Bax inhibitor 1 Antibody (20F565) [NBP2-24912]
Immunohistochemistry-Paraffin: Bax inhibitor 1 Antibody (20F565) [NBP2-24912] - Formalin-fixed, paraffin-embedded human brain stained with Bi-1 antibody at 0.5 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.Applications for Bax inhibitor 1 Antibody (20F565)
Application
Recommended Usage
Immunohistochemistry-Paraffin
0.5-5 ug/ml
Western Blot
1-5 ug/ml
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Bax inhibitor 1
Alternate Names
BAXI1, BI1, BI-1Transmembrane BAX inhibitor motif-containing protein 6, TEGTbax inhibitor 1, testis enhanced gene transcript, Testis-enhanced gene transcript protein, transmembrane BAX inhibitor motif containing 6
Gene Symbol
TMBIM6
UniProt
Additional Bax inhibitor 1 Products
Product Documents for Bax inhibitor 1 Antibody (20F565)
Certificate of Analysis
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Product Specific Notices for Bax inhibitor 1 Antibody (20F565)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for Bax inhibitor 1 Antibody (20F565)
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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