BBOX1 Antibody (OTI4F9) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-71372

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI4F9

Format

Azide and BSA Free
View all BBOX1 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human BBOX1(NP_003977) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

44.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for BBOX1 Antibody (OTI4F9) - Azide and BSA Free

Western Blot: BBOX1 Antibody (OTI4F9)Azide and BSA Free [NBP2-71372]

Western Blot: BBOX1 Antibody (OTI4F9)Azide and BSA Free [NBP2-71372]

Western Blot: BBOX1 Antibody (OTI4F9) - Azide and BSA Free [NBP2-71372] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BBOX1.
Immunocytochemistry/ Immunofluorescence: BBOX1 Antibody (OTI4F9) - Azide and BSA Free [NBP2-71372]

Immunocytochemistry/ Immunofluorescence: BBOX1 Antibody (OTI4F9) - Azide and BSA Free [NBP2-71372]

Immunocytochemistry/Immunofluorescence: BBOX1 Antibody (OTI4F9) - Azide and BSA Free [NBP2-71372] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY BBOX1.
Immunohistochemistry: BBOX1 Antibody (OTI4F9) - Azide and BSA Free [NBP2-71372]

Immunohistochemistry: BBOX1 Antibody (OTI4F9) - Azide and BSA Free [NBP2-71372]

Immunohistochemistry: BBOX1 Antibody (OTI4F9) - Azide and BSA Free [NBP2-71372] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunocytochemistry/ Immunofluorescence: BBOX1 Antibody (OTI4F9) - Azide and BSA Free [NBP2-71372]

Immunocytochemistry/ Immunofluorescence: BBOX1 Antibody (OTI4F9) - Azide and BSA Free [NBP2-71372]

Immunocytochemistry/Immunofluorescence: BBOX1 Antibody (OTI4F9) - Azide and BSA Free [NBP2-71372] - Analysis of HeLa cells.
Immunohistochemistry: BBOX1 Antibody (OTI4F9) - Azide and BSA Free [NBP2-71372]

Immunohistochemistry: BBOX1 Antibody (OTI4F9) - Azide and BSA Free [NBP2-71372]

Immunohistochemistry: BBOX1 Antibody (OTI4F9) - Azide and BSA Free [NBP2-71372] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: BBOX1 Antibody (OTI4F9) - Azide and BSA Free [NBP2-71372]

Immunohistochemistry: BBOX1 Antibody (OTI4F9) - Azide and BSA Free [NBP2-71372]

Immunohistochemistry: BBOX1 Antibody (OTI4F9) - Azide and BSA Free [NBP2-71372] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Applications for BBOX1 Antibody (OTI4F9) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:4000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: BBOX1

BBOX1 encodes gamma butyrobetaine hydroxylase which catalyzes the formation of L-carnitine from gamma-butyrobetaine, the last step in the L-carnitine biosynthetic pathway. Carnitine is essential for the transport of activated fatty acids across the mitochondrial membrane during mitochondrial beta-oxidation.

Alternate Names

2-oxoglutarate dioxygenase, 2-oxoglutarate dioxygenase 1, BBHgamma-butyrobetaine, BBOXEC 1.14.11.1, butyrobetaine (gamma), 2-oxoglutarate dioxygenase (gamma-butyrobetainehydroxylase) 1, gamma-BBH, Gamma-butyrobetaine, gamma-butyrobetaine dioxygenase, Gamma-butyrobetaine hydroxylase, G-BBH

Gene Symbol

BBOX1

Additional BBOX1 Products

Product Documents for BBOX1 Antibody (OTI4F9) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for BBOX1 Antibody (OTI4F9) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for BBOX1 Antibody (OTI4F9) - Azide and BSA Free

There are currently no reviews for this product. Be the first to review BBOX1 Antibody (OTI4F9) - Azide and BSA Free and earn rewards!

Have you used BBOX1 Antibody (OTI4F9) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies