BBOX1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-32327

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human BBOX1. The exact sequence is proprietary.

Localization

Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for BBOX1 Antibody - BSA Free

Western Blot: BBOX1 Antibody [NBP1-32327]

Western Blot: BBOX1 Antibody [NBP1-32327]

Western Blot: BBOX1 Antibody [NBP1-32327] - Sample(30 ug of whole cell lysate)A:MOLT4 7. 5% SDS PAGE, antibody diluted at 1:500.
Immunocytochemistry/ Immunofluorescence: BBOX1 Antibody [NBP1-32327]

Immunocytochemistry/ Immunofluorescence: BBOX1 Antibody [NBP1-32327]

Immunocytochemistry/Immunofluorescence: BBOX1 Antibody [NBP1-32327] - Analysis of methanol-fixed A431, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: BBOX1 Antibody [NBP1-32327]

Immunohistochemistry-Paraffin: BBOX1 Antibody [NBP1-32327]

Immunohistochemistry-Paraffin: BBOX1 Antibody [NBP1-32327] - mouse kidney. BBOX1 antibody diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: BBOX1 Antibody [NBP1-32327]

Western Blot: BBOX1 Antibody [NBP1-32327]

Western Blot: BBOX1 Antibody [NBP1-32327] - Sample (50 ug of whole cell lysate) A: mouse liver 10% SDS PAGE, antibody diluted at 1:500.
Immunohistochemistry-Paraffin: BBOX1 Antibody [NBP1-32327]

Immunohistochemistry-Paraffin: BBOX1 Antibody [NBP1-32327]

Immunohistochemistry-Paraffin: BBOX1 Antibody [NBP1-32327] - Ca922 xenograft. BBOX1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Applications for BBOX1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: BBOX1

This gene encodes gamma butyrobetaine hydroxylase which catalyzes the formation of L-carnitine from gamma-butyrobetaine, the last step in the L-carnitine biosynthetic pathway. Carnitine is essential for the transport of activated fatty acids across the mitochondrial membrane during mitochondrial beta-oxidation.

Alternate Names

2-oxoglutarate dioxygenase, 2-oxoglutarate dioxygenase 1, BBHgamma-butyrobetaine, BBOXEC 1.14.11.1, butyrobetaine (gamma), 2-oxoglutarate dioxygenase (gamma-butyrobetainehydroxylase) 1, gamma-BBH, Gamma-butyrobetaine, gamma-butyrobetaine dioxygenase, Gamma-butyrobetaine hydroxylase, G-BBH

Gene Symbol

BBOX1

Additional BBOX1 Products

Product Documents for BBOX1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for BBOX1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for BBOX1 Antibody - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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