BBOX1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-88695

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (94%), Rat (95%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all BBOX1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DANNVAYTTGKLSFHTDYPALHHPPGVQLLHCIKQTVTGGDSEIVDGFNVCQKLKKNNPQAFQILSSTFVDFTDIGVDYCDFSVQSKHKIIELDDKGQVVRINFNNATRDTIFDVPVERVQPFYAALKEFVDLMNSKESKLT

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BBOX1 Antibody - BSA Free

Immunohistochemistry-Paraffin: BBOX1 Antibody [NBP1-88695]

Immunohistochemistry-Paraffin: BBOX1 Antibody [NBP1-88695]

Immunohistochemistry-Paraffin: BBOX1 Antibody [NBP1-88695] - Analysis in human kidney and skeletal muscle tissues using NBP1-88695 antibody. Corresponding BBOX1 RNA-seq data are presented for the same tissues.
Western Blot: BBOX1 Antibody [NBP1-88695]

Western Blot: BBOX1 Antibody [NBP1-88695]

Western Blot: BBOX1 Antibody [NBP1-88695] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue
Immunohistochemistry-Paraffin: BBOX1 Antibody [NBP1-88695]

Immunohistochemistry-Paraffin: BBOX1 Antibody [NBP1-88695]

Immunohistochemistry-Paraffin: BBOX1 Antibody [NBP1-88695] - Staining of human tonsil shows no positivity in lymphoid cells as expected.
Immunohistochemistry-Paraffin: BBOX1 Antibody [NBP1-88695]

Immunohistochemistry-Paraffin: BBOX1 Antibody [NBP1-88695]

Immunohistochemistry-Paraffin: BBOX1 Antibody [NBP1-88695] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: BBOX1 Antibody [NBP1-88695]

Immunohistochemistry-Paraffin: BBOX1 Antibody [NBP1-88695]

Immunohistochemistry-Paraffin: BBOX1 Antibody [NBP1-88695] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: BBOX1 Antibody [NBP1-88695]

Immunohistochemistry-Paraffin: BBOX1 Antibody [NBP1-88695]

Immunohistochemistry-Paraffin: BBOX1 Antibody [NBP1-88695] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.

Applications for BBOX1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BBOX1

BBOX1 encodes gamma butyrobetaine hydroxylase which catalyzes the formation of L-carnitine from gamma-butyrobetaine, the last step in the L-carnitine biosynthetic pathway. Carnitine is essential for the transport of activated fatty acids across the mitochondrial membrane during mitochondrial beta-oxidation.

Alternate Names

2-oxoglutarate dioxygenase, 2-oxoglutarate dioxygenase 1, BBHgamma-butyrobetaine, BBOXEC 1.14.11.1, butyrobetaine (gamma), 2-oxoglutarate dioxygenase (gamma-butyrobetainehydroxylase) 1, gamma-BBH, Gamma-butyrobetaine, gamma-butyrobetaine dioxygenase, Gamma-butyrobetaine hydroxylase, G-BBH

Gene Symbol

BBOX1

Additional BBOX1 Products

Product Documents for BBOX1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for BBOX1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for BBOX1 Antibody - BSA Free

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Protocols

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FAQs

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