Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Predicted:

Orangutan (100%), Primate (100%), Zebrafish (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 175 and the C-terminus (residue 225) of human Breast Cancer Amplified Sequence 2 using the numbering given in entry NP_005863.1 (GeneID 10286).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BCAS2 Antibody - BSA Free

Western Blot: BCAS2 Antibody [NB110-40681]

Western Blot: BCAS2 Antibody [NB110-40681]

Western Blot: BCAS2 Antibody [NB110-40681] - Detection of Human and Mouse BCAS2 by Western Blot. Samples: Whole cell lysate from MCF7 (5, 15 and 50 ug), and HeLa (50ug), and mouse NIH3T3 (50ug) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BCAS2 antibody NB110-40681 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry-Paraffin: BCAS2 Antibody [NB110-40681]

Immunohistochemistry-Paraffin: BCAS2 Antibody [NB110-40681]

Immunohistochemistry-Paraffin: BCAS2 Antibody [NB110-40681] - Mouse plasmacytoma. Antibody: Affinity purified rabbit anti-BCAS2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB.
Immunohistochemistry-Paraffin: BCAS2 Antibody [NB110-40681]

Immunohistochemistry-Paraffin: BCAS2 Antibody [NB110-40681]

Immunohistochemistry-Paraffin: BCAS2 Antibody [NB110-40681] - Human ovarian carcinoma. Antibody: Affinity purified rabbit anti-BCAS2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB.
Immunoprecipitation: BCAS2 Antibody [NB110-40681]

Immunoprecipitation: BCAS2 Antibody [NB110-40681]

Immunoprecipitation: BCAS2 Antibody [NB110-40681] - Detection of human BCAS2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from MCF-7 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BCAS2 antibody NB110-40681 (lot NB110-40681-2) used for IP at 6 ug per reaction. BCAS2 was also immunoprecipitated by a previous lot of this antibody (lot NB110-40681-1) and another rabbit anti-BCAS2 antibody. For blotting immunoprecipitated BCAS2, NB110-40681 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for BCAS2 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:1000-1:5000

Immunohistochemistry-Paraffin

1:1000-1:5000

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Reviewed Applications

Read 1 review rated 5 using NB110-40681 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: BCAS2

BCAS2 is a Ubiquitously expressed nuclear protein associated with the splicesome. The deduced 225-amino acid protein contains a central potential N-glycosylation site and multiple N-terminal potential phosphorylation sites. Northern and Southern blot analyses demonstrates expression of a 1.5-kb transcript at various levels in multiple breast cancer cell lines, with greatest amplification in BT-20 and MCF-7 cells.

Alternate Names

breast carcinoma amplified sequence 2, Breast carcinoma-amplified sequence 2, DAM1DNA amplified in mammary carcinoma 1 protein, pre-mRNA-splicing factor SPF27, Snt309, SPF27, spliceosome associated protein, amplified in breast cancer, Spliceosome-associated protein SPF 27

Entrez Gene IDs

10286 (Human); 68183 (Mouse)

Gene Symbol

BCAS2

UniProt

O75934 (Human)

Additional BCAS2 Products

Product Documents for BCAS2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for BCAS2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for BCAS2 Antibody - BSA Free

Customer Reviews for BCAS2 Antibody - BSA Free (1)

5 out of 5
1 Customer Rating
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  • Name: Anonymous
    Application: Immunoprecipitation
    Sample Tested: HeLa NE
    Species: Human
    Verified Customer | Posted 10/18/2010

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