BCAT1 Antibody (OTI3F5)

Western Blot: BCAT1 Antibody (3F5) [NBP2-01826] Analysis of extracts (35ug) from 9 different cell lines by using anti-BCAT1 monoclonal antibody.

Western Blot: BCAT1 Antibody (OTI3F5) [NBP2-01826]

Western Blot: BCAT1 Antibody (3F5) [NBP2-01826] - Analysis of extracts (10ug) from 9 Human tissue by using anti-BCAT1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: Colon)

Immunohistochemistry-Paraffin: BCAT1 Antibody (OTI3F5) [NBP2-01826]

Immunohistochemistry-Paraffin: BCAT1 Antibody (3F5) [NBP2-01826] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-BCAT1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: BCAT1 Antibody (OTI3F5) [NBP2-01826]

Immunohistochemistry-Paraffin: BCAT1 Antibody (3F5) [NBP2-01826] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-BCAT1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: BCAT1 Antibody (OTI3F5) [NBP2-01826]

Immunohistochemistry-Paraffin: BCAT1 Antibody (3F5) [NBP2-01826] - Staining of paraffin-embedded Human lymphoma tissue using anti-BCAT1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: BCAT1 Antibody (OTI3F5) [NBP2-01826]

Immunohistochemistry-Paraffin: BCAT1 Antibody (3F5) [NBP2-01826] - Staining of paraffin-embedded Human pancreas tissue using anti-BCAT1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: BCAT1 Antibody (OTI3F5) [NBP2-01826]

Immunohistochemistry-Paraffin: BCAT1 Antibody (3F5) [NBP2-01826] - Staining of paraffin-embedded Human prostate tissue using anti-BCAT1 mouse monoclonal antibody.

BCAT1 Antibody (OTI3F5)[NBP2-01826]

BCAT1 Antibody (OTI3F5)[NBP2-01826]Simple Western� analysis of endogenous protein IFIT3 from THP-1 lysates (0.1 mg/mL) using IFIT3 Mouse Monoclonal Antibody. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess� Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12�230 kDa Separation Module.

Western Blot: BCAT1 Antibody (OTI3F5) [NBP2-01826] -

BCAAs promote the phosphorylation of Tau(A–C) Bcat1 mRNA level is decreased in the brain tissues of diabetic, aged, or 3xTg AD mice (n=5). **P<0.01 by unpaired Student’s t-test. (D) Representative Western blot and quantitative results showing BCAT1 protein level are decreased in the brain tissues of AD mice (n=4). ***P<0.001 by unpaired Student’s t-test. (E) BCAA diet does not affect the content of amyloid beta 42 (A beta 42) in the brain tissues of AD mice (n=5). (F) Representative Western blot and quantitative results showing BCAA diet increase the level of phosphorylated Tau protein in the brain tissues of AD mice (n=4). ***p<0.001 by unpaired Student’s t-test. (G) Relative Western blot and quantitative results showing leucine increase the level of phosphorylated Tau protein in the neurons isolated from mice. The neurons were isolated from the 3xTg mice and treated with leucine (1 mM), isoleucine (1 mM), or valine (1 mM) for 24 h. The experiments were repeated for three times. ***P<0.001 by one-way ANOVA followed by Bonferroni post-hoc test. (H) Bcat1 knockdown increased the level of phosphorylated Tau protein in the neurons isolated from mice. The neurons were isolated from the 3xTg mice and infected with lentivirus carrying indicated shRNAs for 48 h. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29802157), licensed under a CC-BY license. Not internally tested by Novus Biologicals.