BCKDK Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-15553

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Predicted:

Bovine (98%), Rhesus Macaque (98%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all BCKDK Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human BCKDK. The exact sequence is proprietary.

Reactivity Notes

Zebrafish (85%), Xenopus laevis (89%).

Localization

Mitochondrion matrix

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for BCKDK Antibody - BSA Free

Western Blot: BCKDK Antibody [NBP2-15553]

Western Blot: BCKDK Antibody [NBP2-15553]

Western Blot: BCKDK Antibody [NBP2-15553] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with BCKDK antibody [N1C2] diluted at 1:1000.
Immunohistochemistry-Paraffin: BCKDK Antibody [NBP2-15553]

Immunohistochemistry-Paraffin: BCKDK Antibody [NBP2-15553]

Immunohistochemistry-Paraffin: BCKDK Antibody [NBP2-15553] - Immunohistochemical analysis of paraffin-embedded gastric ca, using antibody at 1:500 dilution.
Western Blot: BCKDK Antibody [NBP2-15553]

Western Blot: BCKDK Antibody [NBP2-15553]

Western Blot: BCKDK Antibody [NBP2-15553] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 10% SDS PAGE gel, diluted at 1:1000.
BCKDK Antibody

Western Blot: BCKDK Antibody [NBP2-15553] -

Western Blot: BCKDK Antibody [NBP2-15553] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with BCKDK antibody [N1C2] (NBP2-15553) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for BCKDK Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: BCKDK

Catalyzes the phosphorylation and inactivation of the branched-chain alpha-ketoacid dehydrogenase complex, the key regulatory enzyme of the valine, leucine and isoleucine catabolic pathways. Key enzyme that regulate the activity state of the BCKD complex.

Alternate Names

BCKDHKIN, branched chain alpha-ketoacid dehydrogenase kinase, branched chain ketoacid dehydrogenase kinase, EC 2.7.11, EC 2.7.11.4, mitochondrial

Gene Symbol

BCKDK

UniProt

O14874 (Human)

Additional BCKDK Products

Product Documents for BCKDK Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for BCKDK Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for BCKDK Antibody - BSA Free

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Protocols

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FAQs

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