Bcl G Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-17024

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Bcl G Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DDDLNTIEFKILAYYTRHHVFKSTPALFSPKLLRTRSLSQRGLGNCSANESWTEVSWPCRNSQSSEKAINLGKKKSSWKAFFGVVEKEDSQSTPAKVSAQG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Bcl G Antibody - BSA Free

Western Blot: Bcl G Antibody [NBP3-17024]

Western Blot: Bcl G Antibody [NBP3-17024]

Western Blot: Bcl G Antibody [NBP3-17024] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: RT4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: Tonsil
Immunocytochemistry/ Immunofluorescence: Bcl G Antibody [NBP3-17024]

Immunocytochemistry/ Immunofluorescence: Bcl G Antibody [NBP3-17024]

Immunocytochemistry/Immunofluorescence: Bcl G Antibody [NBP3-17024] - Analysis in human testis and placenta tissues using Anti-BCL2L14 antibody. Corresponding BCL2L14 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Bcl G Antibody [NBP3-17024]

Immunohistochemistry-Paraffin: Bcl G Antibody [NBP3-17024]

Immunohistochemistry-Paraffin: Bcl G Antibody [NBP3-17024] - Staining of human testis shows high expression.
Immunohistochemistry-Paraffin: Bcl G Antibody [NBP3-17024]

Immunohistochemistry-Paraffin: Bcl G Antibody [NBP3-17024]

Immunohistochemistry-Paraffin: Bcl G Antibody [NBP3-17024] - Staining of human placenta shows low expression as expected.

Applications for Bcl G Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Bcl G

Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bcl-G. The mRNA of Bcl-G encodes 2 isoforms, Bcl-GL, which is widely expressed in multiple tissues, and Bcl-GS, which is only found in testis. The Bcl-GS protein is predominantly localized to cytoplasmic organelles whereas Bcl-GL was distributed throughout the cytosol. Overexpression of either protein induced apoptosis, although Bcl-GS was far more potent than Bcl-GS. Apoptosis induction was dependent on the BH3 domain and could be suppressed by co-expression with the anti-apoptotic Bcl-XL protein.

Alternate Names

apoptosis facilitator Bcl-2-like protein 14, Apoptosis regulator Bcl-G, Bcl2-L-14, BCL2-like 14 (apoptosis facilitator), BCL-G, BCLGbcl2-L-14

Gene Symbol

BCL2L14

Additional Bcl G Products

Product Documents for Bcl G Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Bcl G Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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