BCL11B Antibody - BSA Free
Novus Biologicals | Catalog # NB600-263
![Western Blot: BCL11B Antibody [NB600-263]](https://resources.rndsystems.com/images/products/BCL11B-Antibody-Western-Blot-NB600-263-img0006.jpg "Western Blot: BCL11B Antibody [NB600-263]")
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
A synthetic peptide which maps to a region between residues 500 and 550 of human B-cell CLL/lymphoma 11B using the numbering given in Swiss-Prot entry Q9C0K0 (GeneID 64919).
Specificity
NB600-263 is specific for human Bcl-11b protein.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for BCL11B Antibody - BSA Free
Western Blot: BCL11B Antibody [NB600-263]
Western Blot: BCL11B Antibody [NB600-263] - Detection of Human Bcl11b by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Bcl11b antibody NB600-263 used for WB at 0.066 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.![Immunohistochemistry-Paraffin: BCL11B Antibody [NB600-263]](https://resources.rndsystems.com/images/products/BCL11B-Antibody-Immunohistochemistry-NB600-263-img0004.jpg "Immunohistochemistry-Paraffin: BCL11B Antibody [NB600-263]")
Immunohistochemistry-Paraffin: BCL11B Antibody [NB600-263]
Immunohistochemistry-Paraffin: BCL11B Antibody [NB600-263] - Section of human stomach carcinoma. Antibody: Affinity purified rabbit anti-Bcl11b used at a dilution of 1:1,000 (1ug/ml). Detection: DAB![Immunoprecipitation: BCL11B Antibody [NB600-263]](https://resources.rndsystems.com/images/products/BCL11B-Antibody-Immunoprecipitation-NB600-263-img0001.jpg "Immunoprecipitation: BCL11B Antibody [NB600-263]")
Immunoprecipitation: BCL11B Antibody [NB600-263]
Immunoprecipitation: BCL11B Antibody [NB600-263] - Detection of Human Bcl11b on HeLa whole cell lysate using NB600-263. Bcl11b was also immunoprecipitated using affinity purified rabbit anti-Bcl11b antibodies NB600-262 and NB600-264.Applications for BCL11B Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:500 - 1:2000
Immunohistochemistry-Paraffin
1:500 - 1:2000
Immunoprecipitation
1-5 ug/mg lysate
Western Blot
1:5000-1:15000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: BCL11B
Alternate Names
B-cell CLL/lymphoma 11B, B-cell CLL/lymphoma 11B (zinc finger protein), B-cell CLL/lymphoma 11B/T-cell receptor delta constant region fusion protein, B-cell lymphoma/leukaemia 11B, B-cell lymphoma/leukemia 11B, BCL-11B, BCL11B/TRDC fusion, COUP-TF-interacting protein 2, CTIP-2, CTIP2ZNF856B, hRit1, hRIT1-alpha, Radiation-induced tumor suppressor gene 1 protein, RIT1, zinc finger protein hRit1 alpha
Entrez Gene IDs
64919 (Human)
Gene Symbol
BCL11B
UniProt
Additional BCL11B Products
Product Documents for BCL11B Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for BCL11B Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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