Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all BCL11B Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide which maps to a region between residues 850 and the C-terminus (residue 894) of human B-cell CLL/lymphoma 11B using the numbering given in Swiss-Prot entry Q9C0K0 (GeneID 64919).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BCL11B Antibody - BSA Free

Western Blot: BCL11B Antibody [NB600-264]

Western Blot: BCL11B Antibody [NB600-264]

Western Blot: BCL11B Antibody [NB600-264] - Detection of Human Bcl11b by Western Blot. Samples: Whole cell lysate (50, 15, 5 ug) from Jurkat and (50, 15 ug) MOLT-4 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Bcl11b antibody NB600-264 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunohistochemistry: BCL11B Antibody [NB600-264]

Immunohistochemistry: BCL11B Antibody [NB600-264]

Immunohistochemistry: BCL11B Antibody [NB600-264] - Detection of human Bcl11b by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-Bcl11b (NB600-264). Detection: DAB
Immunoprecipitation: BCL11B Antibody [NB600-264]

Immunoprecipitation: BCL11B Antibody [NB600-264]

Immunoprecipitation: BCL11B Antibody [NB600-264] - Detection of Human Bcl11b on HeLa whole cell lysate using NB600-264. Bcl11b was also immunoprecipitated using affinity purified rabbit anti-Bcl11b antibodies NB600-262 and NB600-263.

Applications for BCL11B Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:500-1:2000

Immunohistochemistry-Paraffin

1:500-1:2000

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000-1:0000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: BCL11B

This gene encodes a C2H2-type zinc finger protein and is closely related to BCL11A, a gene whose translocation may be associated with B-cell malignancies. The specific function of this gene has not yet been determined. Two alternatively spliced transcript variants, which encode distinct isoforms, have been reported.

Alternate Names

B-cell CLL/lymphoma 11B, B-cell CLL/lymphoma 11B (zinc finger protein), B-cell CLL/lymphoma 11B/T-cell receptor delta constant region fusion protein, B-cell lymphoma/leukaemia 11B, B-cell lymphoma/leukemia 11B, BCL-11B, BCL11B/TRDC fusion, COUP-TF-interacting protein 2, CTIP-2, CTIP2ZNF856B, hRit1, hRIT1-alpha, Radiation-induced tumor suppressor gene 1 protein, RIT1, zinc finger protein hRit1 alpha

Entrez Gene IDs

64919 (Human)

Gene Symbol

BCL11B

UniProt

Q9C0K0 (Human)

Additional BCL11B Products

Product Documents for BCL11B Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for BCL11B Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for BCL11B Antibody - BSA Free

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Protocols

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FAQs

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