BCL11B Antibody - BSA Free
Novus Biologicals | Catalog # NBP2-33549
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Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Predicted:
Rat (98%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence
Cited:
Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: QGNPQHLSQRELITPEADHVEAAILEEDEGLEIEEPSGLGLMVGGPDPDLLTCG
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for BCL11B Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence: BCL11B Antibody [NBP2-33549]
Immunocytochemistry/Immunofluorescence: BCL11B Antibody [NBP2-33549] - Staining of human cell line MCF7 shows localization to nucleus & nucleoli fibrillar center. Antibody staining is shown in green.Immunohistochemistry-Paraffin: BCL11B Antibody [NBP2-33549]
Immunohistochemistry-Paraffin: BCL11B Antibody [NBP2-33549] - Staining of human cerebral cortex shows weak to moderate nuclear positivity in neurons.Immunohistochemistry: BCL11B Antibody [NBP2-33549]
Immunohistochemistry: BCL11B Antibody [NBP2-33549] - Staining of mouse basal forebrain shows nuclear positivity in neurons of caudate putamen.Immunohistochemistry: BCL11B Antibody [NBP2-33549]
Immunohistochemistry: BCL11B Antibody [NBP2-33549] - Staining of mouse nucleus accumbens shows nuclear positivity in neurons.Immunohistochemistry: BCL11B Antibody [NBP2-33549]
Immunohistochemistry: BCL11B Antibody [NBP2-33549] - Staining of mouse parietal association cortex shows nuclear immunoreactivity in neurons.Immunohistochemistry-Paraffin: BCL11B Antibody [NBP2-33549]
Immunohistochemistry-Paraffin: BCL11B Antibody [NBP2-33549] - Staining of human lymph node shows strong nuclear positivity in subsets of non-germinal center cells.Immunohistochemistry: BCL11B Antibody [NBP2-33549]
Immunohistochemistry: BCL11B Antibody [NBP2-33549] - Staining of mouse brain shows moderate to strong positivity in more than 75 % of neurons in the olfactory bulb.Immunohistochemistry-Paraffin: BCL11B Antibody [NBP2-33549]
Immunohistochemistry-Paraffin: BCL11B Antibody [NBP2-33549] - Staining of human pancreas shows no nuclear positivity in exocrine glandular cells as expected.Immunohistochemistry-Paraffin: BCL11B Antibody [NBP2-33549]
Immunohistochemistry-Paraffin: BCL11B Antibody [NBP2-33549] - Staining of human skin shows moderate to strong nuclear positivity in epidermal cells.Applications for BCL11B Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: BCL11B
Alternate Names
B-cell CLL/lymphoma 11B, B-cell CLL/lymphoma 11B (zinc finger protein), B-cell CLL/lymphoma 11B/T-cell receptor delta constant region fusion protein, B-cell lymphoma/leukaemia 11B, B-cell lymphoma/leukemia 11B, BCL-11B, BCL11B/TRDC fusion, COUP-TF-interacting protein 2, CTIP-2, CTIP2ZNF856B, hRit1, hRIT1-alpha, Radiation-induced tumor suppressor gene 1 protein, RIT1, zinc finger protein hRit1 alpha
Gene Symbol
BCL11B
UniProt
Additional BCL11B Products
Product Documents for BCL11B Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for BCL11B Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for BCL11B Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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