BCL2L12 Antibody (4Z2Z8)

Name: BCL2L12 Antibody (4Z2Z8)
Brand: Novus Biologicals
SKU: NBP3-15930
Price: 389 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-15930

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4Z2Z8 expressed in HEK293

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Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 200-300 of human BCL2L12 (Q9HB09). GPPSTEKEAILRRLVALLEEEAEVINQKLASDPALRSKLVRLSSDSFARLVELFCSRDDSSRPSRACPGPPPPSPEPLARLALAMELSRRVAGLGGTLAGL

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BCL2L12 Antibody (4Z2Z8)

Western Blot: BCL2L12 Antibody (4Z2Z8) [NBP3-15930]

Western Blot: BCL2L12 Antibody (4Z2Z8) [NBP3-15930] - Western blot analysis of extracts of various cell lines, using BCL2L12 Rabbit mAb (NBP3-15930) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Immunohistochemistry-Paraffin: BCL2L12 Antibody (4Z2Z8) [NBP3-15930]

Immunohistochemistry-Paraffin: BCL2L12 Antibody (4Z2Z8) [NBP3-15930] - Immunohistochemistry of paraffin-embedded human esophageal cancer using BCL2L12 Rabbit mAb (NBP3-15930) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunoprecipitation: BCL2L12 Antibody (4Z2Z8) [BCL2L12] -

Immunoprecipitation: BCL2L12 Antibody (4Z2Z8) [BCL2L12] - Immunoprecipitation analysis of 300 ug extracts of PC-3 cells using 3 ug BCL2L12 antibody. Western blot was performed from the immunoprecipitate using BCL2L12 antibody at a dilution of 1:500.

Applications for BCL2L12 Antibody (4Z2Z8)

Application

Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunoprecipitation

1:100 - 1:500

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: BCL2L12

BCL2L12 belongs to the BCL-2 protein family which is involved in several cellular activities. This protein is known to have interaction with BCL2L1, HSPA1A, and HSPA1B. Expression of BCL2L12 results in the resistance to apoptosis.

Alternate Names

Bcl-2 related proline-rich protein, bcl2-L-12, BCL2-like 12 (proline rich), bcl-2-like protein 12, Bcl-2-related proline-rich protein, BPR, MGC120313, MGC120314, MGC120315

Gene Symbol

BCL2L12

Additional BCL2L12 Products

Product Documents for BCL2L12 Antibody (4Z2Z8)

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Certificate of Analysis

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Product Specific Notices for BCL2L12 Antibody (4Z2Z8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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