beta 2-Microglobulin Antibody

Novus Biologicals | Catalog # NBP2-15566

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Monkey

Predicted:

Chimpanzee (100%), Rhesus Macaque (92%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all beta 2-Microglobulin Primary Antibodies »

Product Specifications

Immunogen

Full length human beta 2-Microglobulin Recombinant protein.

Reactivity Notes

Marmoset (100%).

Localization

Secreted

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

14 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit beta 2-Microglobulin Antibody (NBP2-15566) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for beta 2-Microglobulin Antibody

Western Blot: beta 2-Microglobulin Antibody [NBP2-15566]

Western Blot: beta 2-Microglobulin Antibody [NBP2-15566]

Western Blot: beta 2-Microglobulin Antibody [NBP2-15566] - Lane 1: HEK cells. Lane 2: HEK cells with siRNA against beta 2-Microglobulin antibody. Lane 3: HEK cells stimulated for increased beta 2-Microglobulin expression. Image from verified customer review.
Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody [NBP2-15566]

Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody [NBP2-15566]

Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody [NBP2-15566] - Mouse spleen. B2M stained by Beta 2 Microglobulin antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: beta 2-Microglobulin Antibody [NBP2-15566]

Western Blot: beta 2-Microglobulin Antibody [NBP2-15566]

Western Blot: beta 2-Microglobulin Antibody [NBP2-15566] - Rat tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with beta 2 Microglobulin antibody diluted at 1:500.
Western Blot: beta 2-Microglobulin Antibody [NBP2-15566]

Western Blot: beta 2-Microglobulin Antibody [NBP2-15566]

Western Blot: beta 2-Microglobulin Antibody [NBP2-15566] - Mouse plasma (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with beta 2 Microglobulin antibody diluted at 1:1500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: beta 2-Microglobulin Antibody [NBP2-15566]

Western Blot: beta 2-Microglobulin Antibody [NBP2-15566]

Western Blot: beta 2-Microglobulin Antibody [NBP2-15566] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with beta 2-Microglobulin antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody [NBP2-15566]

Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody [NBP2-15566]

Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody [NBP2-15566] - Cal27 xenograft. beta 2 Microglobulin antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody [NBP2-15566]

Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody [NBP2-15566]

Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody [NBP2-15566] - Human endometrial cancer. beta 2 Microglobulin antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunoprecipitation: beta 2-Microglobulin Antibody [NBP2-15566]

Immunoprecipitation: beta 2-Microglobulin Antibody [NBP2-15566]

Immunoprecipitation: beta 2-Microglobulin Antibody [NBP2-15566] - HeLa whole cell extract A. 30 ug HeLa whole cell extract B. Control with 4 ug of preimmune Rabbit IgG C. Immunoprecipitation of B2M protein by 4 ug beta 2 Microglobulin antibody 15 % SDS-PAGE The mmunoprecipitated B2M protein was detected by beta 2 Microglobulin antibody diluted at 1:500.[EasyBlot anti-rabbit IgG was used as a secondary reagent]
beta 2-Microglobulin Antibody

Western Blot: beta 2-Microglobulin Antibody [NBP2-15566] -

Western Blot: beta 2-Microglobulin Antibody [NBP2-15566] - Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with beta 2 Microglobulin antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
beta 2-Microglobulin Antibody

Immunocytochemistry/ Immunofluorescence: beta 2-Microglobulin Antibody [NBP2-15566] -

beta 2 Microglobulin antibody detects beta 2 Microglobulin protein by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: beta 2 Microglobulin stained by beta 2 Microglobulin antibody (NBP2-15566) diluted at 1:500.
Blue: Fluoroshield with DAPI.

Applications for beta 2-Microglobulin Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Assay dependent

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000

Reviewed Applications

Read 1 review rated 5 using NBP2-15566 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: beta 2-Microglobulin

Beta-2-microglobulin is a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells (Gussow et al., 1987 [PubMed 3312414]).[supplied by OMIM]

Alternate Names

B2M, beta 2Microglobulin

Gene Symbol

B2M

UniProt

P61769

Additional beta 2-Microglobulin Products

Product Documents for beta 2-Microglobulin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for beta 2-Microglobulin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Related Research Areas

Cancer Biomarkers

Customer Reviews for beta 2-Microglobulin Antibody (1)

5 out of 5
1 Customer Rating
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used beta 2-Microglobulin Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • beta 2-Microglobulin Antibody
    Name: Lamberto Torralba
    Application: Western Blot
    Sample Tested: 293t HEK and U2OS cells
    Species: Human
    Verified Customer | Posted 05/09/2022
    1. HEK cells 2. HEK cells with siRNA against B2M 3. HEK cells stimulated for increased B2M expression
    beta 2-Microglobulin Antibody NBP2-15566

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies