beta-3 Adrenergic R/ADRB3 Antibody - BSA Free

Novus Biologicals | Catalog # NLS4198

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Human, Rat

Predicted:

Primate (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all beta-3 Adrenergic R/ADRB3 Primary Antibodies »

Product Specifications

Immunogen

Synthetic 20 amino acid peptide from N-terminal extracellular domain of human beta-3 Adrenergic R/ADRB3.

Epitope

N-Terminus extracellular domain

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (100%), Gibbon (95%), Rabbit (85%), Sheep (80%), Goat (80%), Hamster (80%), Feline (80%), Bovine (80%), Equine (80%).

Specificity

Human ADRB3. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Product can be stored undiluted at 4C for up to 1 month.

Scientific Data Images for beta-3 Adrenergic R/ADRB3 Antibody - BSA Free

Immunohistochemistry-Paraffin: beta-3 Adrenergic R/ADRB3 Antibody - BSA Free [NLS4198]

Immunohistochemistry-Paraffin: beta-3 Adrenergic R/ADRB3 Antibody - BSA Free [NLS4198]

Immunohistochemistry-Paraffin: beta-3 Adrenergic R/ADRB3 Antibody [NLS4198] - Analysis of beta-3 Adrenergic R/ADRB3 Antibody with Adipose tissue.
Immunohistochemistry-Paraffin: beta-3 Adrenergic R/ADRB3 Antibody - BSA Free [NLS4198]

Immunohistochemistry-Paraffin: beta-3 Adrenergic R/ADRB3 Antibody - BSA Free [NLS4198]

Immunohistochemistry-Paraffin: beta-3 Adrenergic R/ADRB3 Antibody [NLS4198] - Analysis of anti-ADRB3 antibody with human adipose.
Immunohistochemistry-Paraffin: beta-3 Adrenergic R/ADRB3 Antibody - BSA Free [NLS4198]

Immunohistochemistry-Paraffin: beta-3 Adrenergic R/ADRB3 Antibody - BSA Free [NLS4198]

Immunohistochemistry-Paraffin: beta-3 Adrenergic R/ADRB3 Antibody [NLS4198] - Human Brain, Glioblastoma tissue after heat-induced antigen retrieval.
Immunohistochemistry-Paraffin: beta-3 Adrenergic R/ADRB3 Antibody - BSA Free [NLS4198]

Immunohistochemistry-Paraffin: beta-3 Adrenergic R/ADRB3 Antibody - BSA Free [NLS4198]

Immunohistochemistry-Paraffin: beta-3 Adrenergic R/ADRB3 Antibody [NLS4198] - Human Ovary, Carcinoma tissue after heat-induced antigen retrieval.
Immunohistochemistry-Paraffin: beta-3 Adrenergic R/ADRB3 Antibody - BSA Free [NLS4198]

Immunohistochemistry-Paraffin: beta-3 Adrenergic R/ADRB3 Antibody - BSA Free [NLS4198]

Immunohistochemistry-Paraffin: beta-3 Adrenergic R/ADRB3 Antibody [NLS4198] - Human small intestine tissue after heat-induced antigen retrieval.
Immunohistochemistry-Paraffin: beta-3 Adrenergic R/ADRB3 Antibody - BSA Free [NLS4198]

Immunohistochemistry-Paraffin: beta-3 Adrenergic R/ADRB3 Antibody - BSA Free [NLS4198]

Immunohistochemistry-Paraffin: beta-3 Adrenergic R/ADRB3 Antibody [NLS4198] - Human prostate tissue after heat-induced antigen retrieval.
Immunohistochemistry-Paraffin: beta-3 Adrenergic R/ADRB3 Antibody - BSA Free [NLS4198]

Immunohistochemistry-Paraffin: beta-3 Adrenergic R/ADRB3 Antibody - BSA Free [NLS4198]

Immunohistochemistry-Paraffin: beta-3 Adrenergic R/ADRB3 Antibody [NLS4198] - Analysis of beta-3 Adrenergic R/ADRB3 Antibody with adrenal tissue.

Applications for beta-3 Adrenergic R/ADRB3 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

10 - 15 ug/ml
Application Notes
Use in IHC reported in scientific literature (PMID: 29635716)..

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.

Background: beta-3 Adrenergic R/ADRB3

The Beta-3 Adrenoceptor (ADRB3) is an Adrenergic Receptor that stimulates lipolysis and increases fatty acids in the blood. Stimulation of the beta-3 adrenoceptor leads to lipolysis in white adipocytes and nonshivering thermogenesis in brown fat. The beta-3 adrenoceptor has also been suggested to affect the physiological control of cardiac and vascular contractility; beta-3 adrenoceptor stimulation decreases cardiac contractility through activation of a nitric oxide synthase pathway. A variant of the beta-3 adrenoceptor, Trp64Arg, has been shown to be associated with weight gain (obesity) and susceptibility to non-insulin-dependent diabetes mellitus (NIDDM), but not with coronary artery disease. Trp64Arg variant receptor has been shown to predict a greater tendency to develop abdominal adiposity and high blood pressure with advancing age. ADRB3 has been suggested to be responsible for the negative inotropic effects of catecholamines and may be involved in pathophysiological mechanisms leading to heart failure; ADRB3 is also one of the molecular targets under active research in the treatment of obeisity. Beta-3 adrenoceptor expression has been documented in adipose, heart, and in smooth muscle of digestive and urinary tract organs (bladder, colon, small intestine, stomach, ureter). Utilization of alternate promoters and/or 3-prime untranslated regions may result in tissue-specific regulation of the expression of ADRB3. ESTs have been isolated from heart/melanocyte/uterus and placenta libraries.

Long Name

Beta-3 Adrenergic Receptor

Alternate Names

ADRB3, ADRB3R, B3AR, beta-3 AdrenergicR, beta3 AdrenergicR

Entrez Gene IDs

155 (Human)

Gene Symbol

ADRB3

UniProt

P13945

Additional beta-3 Adrenergic R/ADRB3 Products

Product Documents for beta-3 Adrenergic R/ADRB3 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for beta-3 Adrenergic R/ADRB3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for beta-3 Adrenergic R/ADRB3 Antibody - BSA Free

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Protocols

View specific protocols for beta-3 Adrenergic R/ADRB3 Antibody - BSA Free (NLS4198):

Immunohistochemistry Protocol for Beta 3 Adrenergic Receptor Antibody (NLS4198):
Immunohistochemistry

1. Prepare tissue with formalin fixation and by embedding it in paraffin wax.
2. Make 4 um sections and place on pre-cleaned and charged microscope slides.
3. Heat in a tissue-drying oven for 45 minutes @ 60 degrees Celcius.
4. Deparaffinize the tissues by wash drying the slides in 3 changes of xylene for 5 minutes each @ RT.
5. Rehydrate the tissues by washing the slides in 3 changes of 100% alcohol for 3 minutes each @ RT.
6. Wash the slides in 2 changes of 95% alcohol for 3 minutes each @ RT.
7. Wash the slides in 1 change of 80% alcohol for 3 minutes @ RT.
8. Rinse the slides in gentle running distilled water for 5 minutes @ RT.
9. Perform antigen retrieval by steaming the slides in 0.01M sodium citrate buffer (pH 6.0) @ 99-100 degrees Celcius
for 20 minutes.
10. Remove the slides from the heat and let stand in buffer @ RT for 20 minutes.
11. Rinse the slides in 1X TBS-T for 1 minute @ RT.

**Do not allow the tissues to dry at any time during the staining procedure**

12. Begin the immunostaining by applying a universal protein block for 20 minutes @ RT.
13. Drain protein block from the slides and apply the diluted primary antibody for 45 minutes @ RT.
14. Rinse the slide in 1X TBS-T for 1 minute @ RT.
15. Apply a biotinylated anti-rabbit IgG (H+L) secondary for 30 minutes @ RT.
16. Rinse the slide in 1X TBS-T for 1 minute @ RT.
17. Apply an alkaline phosphatase steptavidin for 30 minutes @ RT.
18. Rinse the slide in 1X TBS-T for 1 minute @ RT.
19. Apply an alkaline phosphatase chromagen substrate for 30 minutes @ RT.
20. Rinse the slide in distilled water for 1 minute @ RT.

**This method should only be used if the chromagen substrate is alcohol insoluble (ie: Vector Red, DAB)**

21. Dehydrate the tissue by washing the slides in 2 changes of 80% alcohol for 1 minute each @ RT.
22. Wash the slides in 2 changes of 95% alcohol for 1 minute each @ RT.
23. Wash the slides in 3 changes of 100% alcohol for 1 minute each @ RT.
24. Wash the slides in 3 changes of xylene for 1 minute each @ RT.
25. Apply cover slip.

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