Q: Are the beta-Actin antibodies validated in Simple Western?

A: Yes, we offer a few beta actin antibodies that have been tested in Simple Western: NBP1-47423, NB600-501, NB600-532, NB600-503, and NB100-56874.

Q: Do Beta-Actin antibodies come in lyophilized format?

A: ACTB antibodies like MAB8969 AND MAB8929 come in lyophilized form.

Q: I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.

A: For homogenized tissue, beta-actin and GAPDH are both fine.

Q: What is the theoretical molecular weight for Beta-Actin antibodies?

A: The TMW for beta Actin antibodies is approximately 42 kDa.

Q: Why is beta actin used as a loading control in blotting ?

A: Beta actin is a highly-expressed protein found in all cells, and is considered a house keeping protein whose expression is necessary for any cell's proper functioning. For this reason it is used as a loading control to confirm that the same amount of total protein is loaded in each lane of an SDS-PAGE gel so that appropriate comparisons can be made between the actual proteins of interest in different samples.

beta-Actin Antibody (4C4)

Novus Biologicals | Catalog # NBP3-16886

(1)

Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas
Related Blogs

Key Product Details

Species Reactivity

Human, Mouse, Rat, Porcine, Chicken, Zebrafish

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4C4 expressed in HEK293

View all beta-Actin Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein of human beta-Actin.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for beta-Actin Antibody (4C4)

Western Blot: beta-Actin Antibody (4C4) [NBP3-16886]

Western Blot: beta-Actin Antibody (4C4) [NBP3-16886] - Analysis of extracts of various cell lines, using beta-Actin antibody (NBP3-16886) at 1:50000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Western Blot: beta-Actin Antibody (4C4) [NBP3-16886]

Western Blot: beta-Actin Antibody (4C4) [NBP3-16886] - Analysis of extracts of HeLa cells, using beta-Actin antibody (NBP3-16886) at 1:10000-1:320000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Immunohistochemistry-Paraffin: beta-Actin Antibody (4C4) [NBP3-16886] -

Immunohistochemistry-Paraffin: beta-Actin Antibody (4C4) [NBP3-16886] - Human liver cancer tissue using beta -Actin Rabbit mAb at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

Western Blot: beta-Actin Antibody (4C4) [beta-Actin] -

Western Blot: beta-Actin Antibody (4C4) [beta-Actin] - Western blot analysis of various lysates using beta-Actin Rabbit mAb at 1:50000 dilution incubated overnight at 4C.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25 ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.

Western Blot: Rabbit beta-Actin mAb (4C4) [NBP3-16886]

Beta actin band (~42kDa) in Pocillopora Acuta (P.acuta) Stony Coral Speices and 3T3L1 as control. Dilution used was 1:1000. Image from a verified customer review.

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Applications for beta-Actin Antibody (4C4)

Application

Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Immunohistochemistry

1:500 - 1:5000

Immunohistochemistry-Paraffin

1:500 - 1:5000

Western Blot

1:10000 -1:100000

Reviewed Applications

Read 1 review rated 5 using NBP3-16886 in the following applications:

Western Blot (1 Review)

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: beta-Actin

Actin is a highly conserved protein involved in cell motility, structure, and integrity. Six functional actin isoforms exist and were named based on their electrophoretic mobility and distribution including alpha-cardiac muscle actin (aCAA), alpha-skeletal muscle actin (aSKA), alpha-smooth muscle actin (aSMA), beta-cytoplasmic actin (bCYA), gamma-cytoplasmic actin (gCYA) and gamma-smooth muscle actin (gSMA) (1). The cytoplasmic isoforms, beta-actin and gamma-actin, are nearly identical, differing by only 4 amino acids at the N-terminus with a theoretical molecular weight of 42 kDa (2). Beta-actin is ubiquitously expressed in eukaryotic cells and considered a housekeeping gene which is frequently used as a loading control for assays involving protein detection, such as Western blotting (3,4).

References

1. Vandekerckhove J, Weber K. 1978. At least six different actins are expressed in a higher mammal: an analysis based on the amino acid sequence of the amino-terminal tryptic peptide. J Mol Biol. 126(4):783-802. PMID: 745245

2. Gimona M, Vandekerckhove J, Goethals M, Herzog M, Lando Z, Small JV. (1994) Beta-actin specific monoclonal antibody. Cell Motil Cytoskeleton. 27(2):108-16. PMID: 8162619

3. Holden VI, Lenio S, Kuick R, Ramakrishnan SK, Shah YM, Bachman MA. (2014) Bacterial siderophores that evade or overwhelm Lipocalin 2 induce HIF-1a and pro-inflammatory cytokine secretion in cultured respiratory epithelial cells Infect Immun. 82(9):3826-36 PMID: 24980968

4. Tsai WL, Yeh PH, Tsai CY, Ting CT, Chiu YH, Tao MH, Li WC, Hung SC. (2016) Efficient Programming of Human Mesenchymal Stem Cell Derived Hepatocytes by Epigenetic Regulations. J. Gastroenterol. Hepatol. 32(1):261-269. PMID: 27218433

Alternate Names

ACTB, betaActin

Gene Symbol

ACTB

Additional beta-Actin Products

Product Documents for beta-Actin Antibody (4C4)

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Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for beta-Actin Antibody (4C4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Adhesion Molecules on Endothelial Cells

Customer Reviews for beta-Actin Antibody (4C4) (1)

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beta-Actin Antibody (4C4)

Name: Anonymous

Application: Western Blot

Sample Tested: Protein Lysate from Stony coral tissue and Protein Lysate from Pocillopora acuta Stony coral tissue

Species: Pocillopora Damicornis and Pocillopora Acuta

Verified Customer | Posted 08/06/2025

Beta actin band (~42kDa) in Pocillopora Acuta (P.acuta) Stony Coral Speices and 3T3L1 as control. Dilution used was 1:1000.

Western blot using PVDF membrane

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for beta-Actin Antibody (4C4)

Showing 1 - 5 of 5 FAQs Showing All

Q: Are the beta-Actin antibodies validated in Simple Western?

A: Yes, we offer a few beta actin antibodies that have been tested in Simple Western: NBP1-47423, NB600-501, NB600-532, NB600-503, and NB100-56874.
Q: Do Beta-Actin antibodies come in lyophilized format?

A: ACTB antibodies like MAB8969 AND MAB8929 come in lyophilized form.
Q: I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.

A: For homogenized tissue, beta-actin and GAPDH are both fine.
Q: What is the theoretical molecular weight for Beta-Actin antibodies?

A: The TMW for beta Actin antibodies is approximately 42 kDa.
Q: Why is beta actin used as a loading control in blotting ?

A: Beta actin is a highly-expressed protein found in all cells, and is considered a house keeping protein whose expression is necessary for any cell's proper functioning. For this reason it is used as a loading control to confirm that the same amount of total protein is loaded in each lane of an SDS-PAGE gel so that appropriate comparisons can be made between the actual proteins of interest in different samples.
Q: Are the beta-Actin antibodies validated in Simple Western?

A: Yes, we offer a few beta actin antibodies that have been tested in Simple Western: NBP1-47423, NB600-501, NB600-532, NB600-503, and NB100-56874.
Q: Do Beta-Actin antibodies come in lyophilized format?

A: ACTB antibodies like MAB8969 AND MAB8929 come in lyophilized form.
Q: I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.

A: For homogenized tissue, beta-actin and GAPDH are both fine.
Q: What is the theoretical molecular weight for Beta-Actin antibodies?

A: The TMW for beta Actin antibodies is approximately 42 kDa.
Q: Why is beta actin used as a loading control in blotting ?

A: Beta actin is a highly-expressed protein found in all cells, and is considered a house keeping protein whose expression is necessary for any cell's proper functioning. For this reason it is used as a loading control to confirm that the same amount of total protein is loaded in each lane of an SDS-PAGE gel so that appropriate comparisons can be made between the actual proteins of interest in different samples.
Q: Are the beta-Actin antibodies validated in Simple Western?

A: Yes, we offer a few beta actin antibodies that have been tested in Simple Western: NBP1-47423, NB600-501, NB600-532, NB600-503, and NB100-56874.
Q: Do Beta-Actin antibodies come in lyophilized format?

A: ACTB antibodies like MAB8969 AND MAB8929 come in lyophilized form.
Q: I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.

A: For homogenized tissue, beta-actin and GAPDH are both fine.
Q: What is the theoretical molecular weight for Beta-Actin antibodies?

A: The TMW for beta Actin antibodies is approximately 42 kDa.
Q: Why is beta actin used as a loading control in blotting ?

A: Beta actin is a highly-expressed protein found in all cells, and is considered a house keeping protein whose expression is necessary for any cell's proper functioning. For this reason it is used as a loading control to confirm that the same amount of total protein is loaded in each lane of an SDS-PAGE gel so that appropriate comparisons can be made between the actual proteins of interest in different samples.
Q: Are the beta-Actin antibodies validated in Simple Western?

A: Yes, we offer a few beta actin antibodies that have been tested in Simple Western: NBP1-47423, NB600-501, NB600-532, NB600-503, and NB100-56874.
Q: Do Beta-Actin antibodies come in lyophilized format?

A: ACTB antibodies like MAB8969 AND MAB8929 come in lyophilized form.
Q: I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.

A: For homogenized tissue, beta-actin and GAPDH are both fine.
Q: What is the theoretical molecular weight for Beta-Actin antibodies?

A: The TMW for beta Actin antibodies is approximately 42 kDa.
Q: Why is beta actin used as a loading control in blotting ?

A: Beta actin is a highly-expressed protein found in all cells, and is considered a house keeping protein whose expression is necessary for any cell's proper functioning. For this reason it is used as a loading control to confirm that the same amount of total protein is loaded in each lane of an SDS-PAGE gel so that appropriate comparisons can be made between the actual proteins of interest in different samples.
Q: Are the beta-Actin antibodies validated in Simple Western?

A: Yes, we offer a few beta actin antibodies that have been tested in Simple Western: NBP1-47423, NB600-501, NB600-532, NB600-503, and NB100-56874.
Q: Do Beta-Actin antibodies come in lyophilized format?

A: ACTB antibodies like MAB8969 AND MAB8929 come in lyophilized form.
Q: I wanted to know which of the two housekeeping genes B-actin or GAPDH can be used for best results. I intend to do an experiment to determine expression of IL-6 and TNF-alpha in liver and kidney tissues.

A: For homogenized tissue, beta-actin and GAPDH are both fine.
Q: What is the theoretical molecular weight for Beta-Actin antibodies?

A: The TMW for beta Actin antibodies is approximately 42 kDa.
Q: Why is beta actin used as a loading control in blotting ?

A: Beta actin is a highly-expressed protein found in all cells, and is considered a house keeping protein whose expression is necessary for any cell's proper functioning. For this reason it is used as a loading control to confirm that the same amount of total protein is loaded in each lane of an SDS-PAGE gel so that appropriate comparisons can be made between the actual proteins of interest in different samples.