beta I Tubulin Antibody (20B1) - BSA Free

Novus Biologicals | Catalog # NBP3-26267

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG Clone # 20B1 expressed in HEK293

Format

BSA Free
View all beta-I Tubulin Primary Antibodies »

Product Specifications

Immunogen

Recombinant Human beta I Tubulin protein [UniProt Q9H4B7]

Clonality

Monoclonal

Host

Mouse

Isotype

IgG

Scientific Data Images for beta I Tubulin Antibody (20B1) - BSA Free

beta I Tubulin Antibody (16E2)

Immunohistochemistry: beta I Tubulin Antibody (16E2) [NBP3-26267] -

Immunohistochemistry: beta I Tubulin Antibody (16E2) [NBP3-26267] - Image of beta I Tubulin Antibody (16E2) diluted at 1:300 and staining in paraffin-embedded human pancreatic cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
beta I Tubulin Antibody (16E2)

Immunohistochemistry: beta I Tubulin Antibody (16E2) [NBP3-26267] -

Immunohistochemistry: beta I Tubulin Antibody (16E2) [NBP3-26267] - Image of beta I Tubulin Antibody (16E2) diluted at 1:300 and staining in paraffin-embedded human pancreatic cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
beta I Tubulin Antibody (16E2)

Western Blot: beta I Tubulin Antibody (16E2) [NBP3-26267] -

Western Blot: beta I Tubulin Antibody (16E2) [NBP3-26267] - Positive Western Blot detected in: K562 whole cell lysate.
All lanes: beta I Tubulin Antibody at 1: 1000
Secondary: Goat polyclonal to mouse IgG at 1/50000 dilution.
Predicted band size: 50 kDa
Observed band size: 50 kDa
beta I Tubulin Antibody (16E2)

Flow Cytometry: beta I Tubulin Antibody (16E2) [NBP3-26267] -

Flow Cytometry: beta I Tubulin Antibody (16E2) [NBP3-26267] - Overlay Peak curve showing NIH/3T3 cells stained with beta I Tubulin Antibody (16E2) (red line) at 1:200. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45 min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG (H+L) at 1/200 dilution for 35 min at 4C. Isotype control antibody (green line) was mouse IgG1 (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

Applications for beta I Tubulin Antibody (20B1) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:50-1:200

Immunohistochemistry

1:50-1:200

Western Blot

1:1000-1:5000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50% Glycerol, 0.01M PBS, PH 7.4

Format

BSA Free

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: beta-I Tubulin

Microtubules are involved in a wide variety of cellular processes, including mitosis, morphogenesis, plateletformation, and mobility of cilia and flagella. Circulating platelets carry a single marginal microtubule coil that iswound in 8 to 12 turns and is responsible for platelet shape. TUBB1 is the major beta-tubulin expressed in plateletsand megakaryocytes and is required for optimal platelet assembly (Wang et al., 1986 (PubMed 3782288); Schulze et al.,2004 (PubMed 15315966)).(supplied by OMIM)

Long Name

Tubulin, Beta 1 Class VI

Alternate Names

betaI Tubulin, TUBB1

Gene Symbol

TUBB1

Additional beta-I Tubulin Products

Product Documents for beta I Tubulin Antibody (20B1) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for beta I Tubulin Antibody (20B1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Acute Myeloid Leukemia
Microtubules

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Protocols

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