Beta Ig-h3/TGFBI Antibody (7Q5O9)

Novus Biologicals | Catalog # NBP3-15994

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 7Q5O9 expressed in HEK293
View all beta IG-H3 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 50-150 of human Beta Ig-h3/TGFBI (Q15582). AVQKVIGTNRKYFTNCKQWYQRKICGKSTVISYECCPGYEKVPGEKGCPAALPLSNLYETLGVVGSTTTQLYTDRTEKLRPEMEGPGSFTIFAPSNEAWAS

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

75 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Beta Ig-h3/TGFBI Antibody (7Q5O9)

Western Blot: Beta Ig-h3/TGFBI Antibody (7Q5O9) [NBP3-15994]

Western Blot: Beta Ig-h3/TGFBI Antibody (7Q5O9) [NBP3-15994]

Western Blot: Beta Ig-h3/TGFBI Antibody (7Q5O9) [NBP3-15994] - Analysis of extracts of various cell lines, using TGF beta induced Beta Ig-h3/TGFBI Rabbit mAb (NBP3-15994) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody (7Q5O9) [NBP3-15994]

Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody (7Q5O9) [NBP3-15994]

Immunohistochemistry-Paraffin: Beta Ig-h3/TGFBI Antibody (7Q5O9) [NBP3-15994] - Human esophageal using TGF beta induced Beta Ig-h3/TGFBI Rabbit mAb (NBP3-15994) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Western Blot: Beta Ig-h3/TGFBI Antibody (7Q5O9) [NBP3-15994]

Western Blot: Beta Ig-h3/TGFBI Antibody (7Q5O9) [NBP3-15994]

Western Blot: Beta Ig-h3/TGFBI Antibody (7Q5O9) [NBP3-15994] - Analysis of extracts of various cell lines, using TGF beta induced Beta Ig-h3/TGFBI Rabbit mAb (NBP3-15994) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Applications for Beta Ig-h3/TGFBI Antibody (7Q5O9)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: beta IG-H3

TGFBI encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. The protein is induced by transforming growth factor-beta and acts to inhibit cell adhesion. Mutations in this gene are associated with multiple types of corneal dystrophy. [provided by RefSeq]

Long Name

TGF-beta Induced Gene H3

Alternate Names

beta IGH3, Kerato-epithelin, RGD-CAP, TGFBI, TGFBIP

Gene Symbol

TGFBI

Additional beta IG-H3 Products

Product Documents for Beta Ig-h3/TGFBI Antibody (7Q5O9)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Beta Ig-h3/TGFBI Antibody (7Q5O9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Beta Ig-h3/TGFBI Antibody (7Q5O9)

There are currently no reviews for this product. Be the first to review Beta Ig-h3/TGFBI Antibody (7Q5O9) and earn rewards!

Have you used Beta Ig-h3/TGFBI Antibody (7Q5O9)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies