beta II Tubulin A Antibody

Novus Biologicals | Catalog # NBP2-15581

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Drosophila

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all beta II Tubulin A Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human beta Tubulin 2. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for beta II Tubulin A Antibody

Immunohistochemistry-Paraffin: beta II Tubulin A Antibody [NBP2-15581]

Immunohistochemistry-Paraffin: beta II Tubulin A Antibody [NBP2-15581]

Immunohistochemistry-Paraffin: beta II Tubulin A Antibody [NBP2-15581] - Mouse duodenum. beta Tubulin 2 antibody dilution: 1:500.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
beta II Tubulin A Antibody

Western Blot: beta II Tubulin A Antibody [NBP2-15581] -

beta Tubulin 2 antibody detects beta Tubulin 2 protein by Western blot analysis.
A. 30 ug Jurkat whole cell lysate/extract
B. 30 ug Raji whole cell lysate/extract
C. 30 ug NCI-H929 whole cell lysate/extract
10 % SDS-PAGE
beta Tubulin 2 antibody (NBP2-15581) dilution: 1:5000
beta II Tubulin A Antibody

Western Blot: beta II Tubulin A Antibody [NBP2-15581] -

beta Tubulin 2 antibody detects beta Tubulin 2 protein by Western blot analysis.
A. 30 ug Neuro2A whole cell lysate/extract
B. 30 ug GL261 whole cell lysate/extract
C. 30 ug C8D30 whole cell lysate/extract
D. 30 ug NIH-3T3 whole cell lysate/extract
E. 30 ug BCL-1 whole cell lysate/extract
F. 30 ug Raw 264.7 whole cell lysate/extract
G. 30 ug C2Cl2 whole cell lysate/extract
10 % SDS-PAGE
beta Tubulin 2 antibody (NBP2-15581) dilution: 1:5000
beta II Tubulin A Antibody

Western Blot: beta II Tubulin A Antibody [NBP2-15581] -

beta Tubulin 2 antibody detects beta Tubulin 2 protein by Western blot analysis.
A. 50 ug rat brain lysate/extract
10 % SDS-PAGE
beta Tubulin 2 antibody (NBP2-15581) dilution: 1:5000
beta II Tubulin A Antibody

Immunocytochemistry/ Immunofluorescence: beta II Tubulin A Antibody [NBP2-15581] -

TUBB2A antibody detects TUBB2A protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde/PBS for 15 min.
Green: TUBB2A protein stained by TUBB2A antibody (NBP2-15581) diluted at 1:500.
Blue: Hoechst 33342 staining.
beta II Tubulin A Antibody

Western Blot: beta II Tubulin A Antibody [NBP2-15581] -

beta Tubulin 2 antibody detects beta Tubulin 2 protein by Western blot analysis.
A. 30 ug U87-MG whole cell lysate/extract
B. 30 ug SK-N-SH whole cell lysate/extract
C. 30 ug IMR32 whole cell lysate/extract
D. 30 ug SK-N-AS whole cell lysate/extract
10 % SDS-PAGE
beta Tubulin 2 antibody (NBP2-15581) dilution: 1:10000
beta II Tubulin A Antibody

Western Blot: beta II Tubulin A Antibody [NBP2-15581] -

beta Tubulin 2 antibody detects beta Tubulin 2 protein by Western blot analysis.
A. 30 ug drosophila lysate/extract
10 % SDS-PAGE
beta Tubulin 2 antibody (NBP2-15581) dilution: 1:2000

Applications for beta II Tubulin A Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: beta II Tubulin A

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.

Long Name

Tubulin beta-2A chain

Alternate Names

TUBB2, TUBB2A

Gene Symbol

TUBB2A

UniProt

Q13885 (Human)

Additional beta II Tubulin A Products

Product Documents for beta II Tubulin A Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for beta II Tubulin A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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