beta-III Tubulin Antibody (CL5813) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-44243

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL5813

Format

Azide and BSA Free
View all beta-III Tubulin Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated using a synthetic peptide of Q13509, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for beta-III Tubulin Antibody (CL5813) - Azide and BSA Free

beta-III Tubulin Antibody (CL5813) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: beta-III Tubulin Antibody [NBP3-44243]

Immunocytochemistry/ Immunofluorescence: beta-III Tubulin Antibody [NBP3-44243]

Staining of A549 cells using the Anti-beta-III Tubulin monoclonal antibody).
beta-III Tubulin Antibody (CL5813) - Azide and BSA Free Immunohistochemistry-Paraffin: beta-III Tubulin Antibody [NBP3-44243]

Immunohistochemistry-Paraffin: beta-III Tubulin Antibody [NBP3-44243]

Staining of human cerebral cortex shows strong positivity in neuropil as well as in neurons.
beta-III Tubulin Antibody (CL5813) - Azide and BSA Free Immunohistochemistry-Paraffin: beta-III Tubulin Antibody [NBP3-44243]

Immunohistochemistry-Paraffin: beta-III Tubulin Antibody [NBP3-44243]

Staining of human cerebellum shows strong positivity in both neurons and neuropil.
beta-III Tubulin Antibody (CL5813) - Azide and BSA Free Immunohistochemistry-Paraffin: beta-III Tubulin Antibody [NBP3-44243]

Immunohistochemistry-Paraffin: beta-III Tubulin Antibody [NBP3-44243]

Staining of human skeletal muscle shows absence of positivity in striated muscle fibers as expected (negative control).
beta-III Tubulin Antibody (CL5813) - Azide and BSA Free Immunohistochemistry-Paraffin: beta-III Tubulin Antibody [NBP3-44243]

Immunohistochemistry-Paraffin: beta-III Tubulin Antibody [NBP3-44243]

Staining of human prostate shows strong positivity in the peripheral nerves.
beta-III Tubulin Antibody (CL5813) - Azide and BSA Free Western Blot: beta-III Tubulin Antibody [NBP3-44243]

Western Blot: beta-III Tubulin Antibody [NBP3-44243]

Analysis in human cell line SH-SY5Y.
beta-III Tubulin Antibody (CL5813) - Azide and BSA Free Immunohistochemistry-Paraffin: beta-III Tubulin Antibody [NBP3-44243]

Immunohistochemistry-Paraffin: beta-III Tubulin Antibody [NBP3-44243]

Staining of mouse embryo E11 shows strong positivity in the developing central and peripheral nervous system.
beta-III Tubulin Antibody (CL5813) - Azide and BSA Free Western Blot: beta-III Tubulin Antibody [NBP3-44243]

Western Blot: beta-III Tubulin Antibody [NBP3-44243]

Analysis in human cerebral cortex tissue.
beta-III Tubulin Antibody (CL5813) - Azide and BSA Free Immunohistochemistry-Paraffin: beta-III Tubulin Antibody [NBP3-44243]

Immunohistochemistry-Paraffin: beta-III Tubulin Antibody [NBP3-44243]

Staining of rat cerebral cortex shows strong positivity in neuronal cell bodies and in neuropil.
beta-III Tubulin Antibody (CL5813) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: beta-III Tubulin Antibody [NBP3-44243]

Immunocytochemistry/ Immunofluorescence: beta-III Tubulin Antibody [NBP3-44243]

Staining of SH-SY5Y cells using the Anti-beta-III Tubulin monoclonal antibody, showing specific staining on microtubules in green. Nuclear probe is visualized in blue.

Applications for beta-III Tubulin Antibody (CL5813) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:50000 - 1:100000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/ Immunofluorescence/IF,Fixation Permeabilization: PFA/Triton X-100

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: beta-III Tubulin

Beta-III Tubulin is abundant in the central and peripheral nervous systems where it is prominently expressed during fetal and postnatal development. Beta III Tubulin is often referred to as TUJ1 because Tuj-1 is the clone name of the original monoclonal antibody. The Tuj1 clone is such a popular neuronal marker that Tuj-1 is now used as a synonym for beta III tubulin. The distribution of Tubulin Beta 3 is neuron-associated, exhibiting distinct temporospatial gradients according to the regional neuroepithelia of origin. However, transient expression of the Tubulin Beta 3 protein is also present in the subventricular zones of the CNS comprising putative neuronal- and/or glial precursor cells, as well as in Kulchitsky neuroendocrine cells of the fetal respiratory epithelium. This temporally restricted, potentially non-neuronal expression may have implications in the identification of presumptive neurons derived from embryonic stem cells. Mutation on beta-tubulin has been linked to anti-cancer drug resistance in lung cancers.

Alternate Names

beta-Tubulin III, Beta3-tubulin, betaIII Tubulin, Class III beta-tubulin, TUBB3, Tubulin Beta 3, Tubulin beta-4, Tubulin beta-III

Gene Symbol

TUBB3

Additional beta-III Tubulin Products

Product Documents for beta-III Tubulin Antibody (CL5813) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for beta-III Tubulin Antibody (CL5813) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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