beta-III Tubulin Antibody (TU-20) - BSA Free

Novus Biologicals | Catalog # NB600-1018

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Canine

Cited:

Human, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # TU-20

Format

BSA Free
View all beta-III Tubulin Primary Antibodies »

Product Specifications

Immunogen

Peptide (C) 441-448 coupled to maleimide-activated keyhole limpet hemocyanin via cysteine added to the N-terminus of the neuron-specific peptide.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions. Rat reactivity reported in scientific literature (PMID: 23984759).

Marker

Neuronal Marker

Specificity

The antibody TU-20 recognizes C-terminal peptide sequence ESESQGPK (aa 441-448) of neuron-specific human betaIII-tubulin.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for beta-III Tubulin Antibody (TU-20) - BSA Free

Immunocytochemistry/ Immunofluorescence: beta-III Tubulin Antibody (TU-20) - BSA Free [NB600-1018]

Immunocytochemistry/ Immunofluorescence: beta-III Tubulin Antibody (TU-20) - BSA Free [NB600-1018]

Immunocytochemistry/Immunofluorescence: beta-III Tubulin Antibody (TU-20) [NB600-1018] - Merged image of co-staining with anti-beta-tubulin (TU-06; green) Superposition of red and green colours provided yellow staining. Nuclei were stained with DNA-binding dye (blue).
Flow Cytometry: beta-III Tubulin Antibody (TU-20) - BSA Free [NB600-1018]

Flow Cytometry: beta-III Tubulin Antibody (TU-20) - BSA Free [NB600-1018]

Flow Cytometry: beta-III Tubulin Antibody (TU-20) [NB600-1018] - An intracellular stain was performed on SH-SY5Y cells with beta-III Tubulin (TU-20) antibody NB600-1018PE (blue) and a matched isotype control NBP2-27287PE (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.
Immunocytochemistry/ Immunofluorescence: beta-III Tubulin Antibody (TU-20) - BSA Free [NB600-1018]

Immunocytochemistry/ Immunofluorescence: beta-III Tubulin Antibody (TU-20) - BSA Free [NB600-1018]

Immunocytochemistry/Immunofluorescence: beta-III Tubulin Antibody (TU-20) [NB600-1018] - Staining of P-19 mouse embryonal carcinoma cell line stimulated to neuronal differentiation by retinoic acid. 2A - Microtubules decorated with neuron-specific anti-betaIII-tubulin (TU-20; red).
Immunocytochemistry/ Immunofluorescence: beta-III Tubulin Antibody (TU-20) - BSA Free [NB600-1018]

Immunocytochemistry/ Immunofluorescence: beta-III Tubulin Antibody (TU-20) - BSA Free [NB600-1018]

Immunocytochemistry/Immunofluorescence: beta-III Tubulin Antibody (TU-20) [NB600-1018] - Staining of Neuro2a mouse neuroblastoma cell line using anti-betaIII-tubulin (TU-20; green; 3 ig/ml). Nuclei were stained with DAPI (blue).
Flow Cytometry: beta-III Tubulin Antibody (TU-20) - BSA Free [NB600-1018]

Flow Cytometry: beta-III Tubulin Antibody (TU-20) - BSA Free [NB600-1018]

Flow Cytometry: beta-III Tubulin Antibody (TU-20) [NB600-1018] - Analysis of PerCP conjugate of NB600-1018. An intracellular stain was performed on SH-SY5Y cells with beta-III Tubulin (TU-20) antibody NB600-1018PCP (blue) and a matched isotype control NBP2-27287PCP (orange). Cells were fixed with 4% PFA and then permea
beta-III Tubulin Antibody (TU-20) - BSA Free Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (TU-20) - BSA Free [NB600-1018]

Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (TU-20) - BSA Free [NB600-1018]

Staining of betaIII tubulin (red) in tissue sections of murine brain expressing GFP in some of its neurons (green). Mouse monoclonal antibody TU-20 (anti-betaIII tubulin) was detected with goat anti-mouse IgG1 conjugated with Alexa Fluor 555.

Applications for beta-III Tubulin Antibody (TU-20) - BSA Free

Application
Recommended Usage

Flow (Intracellular)

1-4 ug/ml

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

10 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Western Blot - Sample preparation: Mix lysate with reducing Laemmli SDS-PAGE sample buffer. Application note: Reducing conditions. Immunohistochemistry-Paraffin sections - Staining technique: Standard ABC technique (DAB+) Pretreatment: 0.1% pepsin (trypsin) in 0.1 M HCl; incubation 30 min in RT; or High temperature citrate buffer antigen retrieval

Reviewed Applications

Read 1 review rated 4 using NB600-1018 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: beta-III Tubulin

The betaIII-tubulin isoform is present dominantly in cells of neuronal origin and it is one of the earliest markers of neuronal differentiation. It is regarded as a specific probe for the cells of neuronal origin as well as for the tumours originating from these cells. The betaIII-tubulin is most abundant in cells of neuronal origin, but was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues.

Alternate Names

beta-Tubulin III, Beta3-tubulin, betaIII Tubulin, Class III beta-tubulin, TUBB3, Tubulin Beta 3, Tubulin beta-4, Tubulin beta-III

Gene Symbol

TUBB3

Additional beta-III Tubulin Products

Product Documents for beta-III Tubulin Antibody (TU-20) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for beta-III Tubulin Antibody (TU-20) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for beta-III Tubulin Antibody (TU-20) - BSA Free

Customer Reviews for beta-III Tubulin Antibody (TU-20) - BSA Free (1)

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  • Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: Mouse Differentiated Neural Cell
    Species: Mouse
    Verified Customer | Posted 09/23/2011

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