beta Tubulin Antibody (D66)

Novus Biologicals | Catalog # NB600-1514

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine, Canine, Chicken, Primate, Rabbit

Cited:

Human

Applications

Validated:

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # D66
View all beta Tubulin Primary Antibodies »

Product Specifications

Immunogen

Sea urchin (Lytechinus pictus) sperm axonemal proteins.

Epitope

The epitope recognized by the antibody is localized in the C-terminal region of beta tubulin (amino acids 427-432 in sea urchin beta 2 tubulin), but is found in all isoforms of beta tubulin.

Reactivity Notes

Cross-reacts with Human, Monkey Mouse, Rat, Cow, Chicken, Chlamydomonas reinhardtii, Dog, Rabbit, Dinoflagellate and Sea Urchin. Not yet tested in other species. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Localization

Cytoplasmic; Major constituent of microtubules.

Marker

Microtubule Marker

Specificity

NB600-1514 recognizes all mouse brain beta tubulin isoforms (beta and beta subpopulations), and sea urchin beta Tubulin.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

~50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for beta Tubulin Antibody (D66)

Western Blot: beta Tubulin Antibody (D66) [NB600-1514]

Western Blot: beta Tubulin Antibody (D66) [NB600-1514]

Western Blot: beta Tubulin Antibody (D66) [NB600-1514] - Cell line lysates were separated on SDS-PAGE and probed with 1 ug/mL Monoclonal Anti-beta Tubulin, Clone: D66. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate.Lanes:1. HeLa; 2. U87; 3. P19; 4. Neuro-2a; 5. PC-12; 6. COS-7; 7. CHO; 8. MDBK; 9. MDCK
Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody (D66) [NB600-1514]

Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody (D66) [NB600-1514]

Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody (D66) [NB600-1514] - SH-SY-5Y cells were fixed and permeabilized with methanol followed by methanol:acetone. Fixed cells were stained with 0.5 ug/mL Monoclonal Anti-beta Tubulin, Clone: D66. The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Western Blot: beta Tubulin Antibody (D66) [NB600-1514]

Western Blot: beta Tubulin Antibody (D66) [NB600-1514]

Western Blot: beta Tubulin Antibody (D66) [NB600-1514] - beta Tubulin antibody Total cell extract of PC-12 was separated on SDS-PAGE. The antibody was developed with Goat Anti-Mouse IgG, alkaline phosphatase conjugate and a chemiluminescent substrate. Lanes 1. 0.5 ug/mL antibody 2. 1 ug/mL antibody
Western Blot: beta Tubulin Antibody (D66) [NB600-1514]

Western Blot: beta Tubulin Antibody (D66) [NB600-1514]

beta-Tubulin-Antibody-D66-Western-Blot-NB600-1514-img0002.jpg

Applications for beta Tubulin Antibody (D66)

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Immunoprecipitation

1:10-1:500

Western Blot

0.5-1 ug/ml
Application Notes
ICC:(3% paraformaldehyde-0.5% Triton X-100 and methanol-acetone. Predicted molecular weight: 50 kDa.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS (pH 7.4) and 1.0% BSA

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: beta Tubulin

beta Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and in the cytoskeleton.

Long Name

Tubulin beta chain

Alternate Names

OK/SW-cl.56, TUBB, TUBB5

Entrez Gene IDs

203068 (Human); 22151 (Mouse); 498736 (Rat)

Gene Symbol

TUBB

Additional beta Tubulin Products

Product Documents for beta Tubulin Antibody (D66)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for beta Tubulin Antibody (D66)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for beta Tubulin Antibody (D66)

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Protocols

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FAQs

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