beta Tubulin Antibody

Novus Biologicals | Catalog # NBP2-15584

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Chicken (100%), Chimpanzee (100%), Porcine (100%), Rhesus Macaque (100%), Xenopus (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all beta Tubulin Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human beta Tubulin. The exact sequence is proprietary.

Marker

Microtubule Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for beta Tubulin Antibody

Western Blot: beta Tubulin Antibody [NBP2-15584]

Western Blot: beta Tubulin Antibody [NBP2-15584]

Western Blot: beta Tubulin Antibody [NBP2-15584] - ample (whole cell lysate) A: 293T 20ug B: 293T 10ug C: 293T 5ug 10% SDS PAGE diluted at 1:10000
Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody [NBP2-15584]

Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody [NBP2-15584]

Immunocytochemistry/Immunofluorescence: beta Tubulin Antibody [NBP2-15584] - Analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: beta Tubulin Antibody [NBP2-15584]

Immunohistochemistry-Paraffin: beta Tubulin Antibody [NBP2-15584]

Immunohistochemistry-Paraffin: beta Tubulin Antibody [NBP2-15584] - HeLa xenograft, using beta Tubulin antibody at 1:500 dilution. Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min
Western Blot: beta Tubulin Antibody [NBP2-15584]

Western Blot: beta Tubulin Antibody [NBP2-15584]

Western Blot: beta Tubulin Antibody [NBP2-15584] - Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE gel, diluted at 1:1000.
Western Blot: beta Tubulin Antibody [NBP2-15584]

Western Blot: beta Tubulin Antibody [NBP2-15584]

Western Blot: beta Tubulin Antibody [NBP2-15584] - Sample (30 ug of whole cell lysate) A: NIH-3T3, B: JC, C: BCL-1, D: C2C12, E: Raw264. 7 10% SDS PAGE NBP2-15584 diluted at 1:1000.

Applications for beta Tubulin Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: beta Tubulin

Beta Tubulin antibodies are useful as loading controls for Western Blotting and ELISA, as well as in studies invlolving cell structure and division. Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and in the cytoskeleton.

Long Name

Tubulin beta chain

Alternate Names

OK/SW-cl.56, TUBB, TUBB5

Gene Symbol

TUBB

Additional beta Tubulin Products

Product Documents for beta Tubulin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for beta Tubulin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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