BICD2 Antibody (10811) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-43683
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Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse, Rat
Predicted:
Bovine (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 10811
Format
Azide and BSA Free
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Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human BICD2. The exact sequence is proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Theoretical MW
94 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for BICD2 Antibody (10811) - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence: BICD2 Antibody (10811) [NBP2-43683]
Immunocytochemistry/Immunofluorescence: BICD2 Antibody (10811) [NBP2-43683] - Analysis of human RPE1 cells using BICD2 antibody. Image from verified customer review.Western Blot: BICD2 Antibody (10811) [NBP2-43683]
Western Blot: BICD2 Antibody (10811) [NBP2-43683] - Analysis of various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with BICD2 antibody diluted by 1:1000.Western Blot: BICD2 Antibody (10811) [NBP2-43683]
Western Blot: BICD2 Antibody (10811) [NBP2-43683] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with BICD2 antibody diluted at 1:1000.Immunocytochemistry/ Immunofluorescence: BICD2 Antibody (10811) [NBP2-43683] -
BICD2 antibody [GT10811] detects BICD2 protein by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: BICD2 stained by BICD2 antibody [GT10811] (NBP2-43683) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.
Blue: Fluoroshield with DAPI.
Western Blot: BICD2 Antibody (10811) [NBP2-43683] -
Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with BICD2 antibody [GT10811] (NBP2-43683) diluted at 1:1000.Applications for BICD2 Antibody (10811) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunoprecipitation
1:100-1:500
Western Blot
1:500-1:3000
Reviewed Applications
Read 1 review rated 5 using NBP2-43683 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: BICD2
Alternate Names
bicaudal D homolog 2 (Drosophila), KIAA0699
Gene Symbol
BICD2
Additional BICD2 Products
Product Documents for BICD2 Antibody (10811) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for BICD2 Antibody (10811) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Customer Reviews for BICD2 Antibody (10811) - Azide and BSA Free (1)
5 out of 5
1 Customer Rating
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Application: ImmunocytochemistrySample Tested: RPE1 cellsSpecies: HumanVerified Customer | Posted 08/23/2022This antibody can clearly detect the Golgi localization of endogenous BICD2.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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