Bim Antibody (1C2H4) - BSA Free
Novus Biologicals | Catalog # NBP1-75511
![Western Blot: Bim Antibody (1C2H4)BSA Free [NBP1-75511]](https://resources.rndsystems.com/images/products/Bim-Antibody-1C2H4-Western-Blot-NBP1-75511-img0003.jpg "Western Blot: Bim Antibody (1C2H4)BSA Free [NBP1-75511]")
Key Product Details
Species Reactivity
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Scientific Data Images for Bim Antibody (1C2H4) - BSA Free
Western Blot: Bim Antibody (1C2H4)BSA Free [NBP1-75511]
Western Blot: Bim Antibody (1C2H4) [NBP1-75511] - Western blot analysis of 5 ng of Bim recombinant protein with Bim antibody at 1 ug/ml.
Immunocytochemistry/ Immunofluorescence: Bim Antibody (1C2H4) - BSA Free [NBP1-75511] -
Immunocytochemistry/ Immunofluorescence: Bim Antibody (1C2H4) - BSA Free [NBP1-75511] - Immunofluorescence of Bim in K562 cells with Bim antibody at 20 u/mL.
Immunocytochemistry/ Immunofluorescence: Bim Antibody (1C2H4) - BSA Free [NBP1-75511] -
Immunocytochemistry/ Immunofluorescence: Bim Antibody (1C2H4) - BSA Free [NBP1-75511] - Immunocytochemistry of Bim in K562 cells with Bim antibody at 10 u/mL.Applications for Bim Antibody (1C2H4) - BSA Free
ELISA
Immunocytochemistry/ Immunofluorescence
Western Blot
Formulation, Preparation, and Storage
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Formulation
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Preservative
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Background: Bim
Alternate Names
Gene Symbol
UniProt
Additional Bim Products
Product Documents for Bim Antibody (1C2H4) - BSA Free
Certificate of Analysis
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Product Specific Notices for Bim Antibody (1C2H4) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Bim Antibody (1C2H4) - BSA Free
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Q: Please recommend which isotype antibody I should use with the NBP1-75511AF647 (Bim-AF647.) The product datasheet only says IgG, but no subclass or product number.
A: This item is a mouse IgG1 antibody. For isotype control, I would recommend NBP1-43319AF647 or NBP2-27287AF647.
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Q: Would you recommend a standard Fix/Perm buffer for Bim staining?
A:
Our lab used our standard ICC protocol for cell staining with this product. We used tween-20 in the permeabilization step as BIM can be found in the endomembrane system and mitochondria.
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Q: Please recommend which isotype antibody I should use with the NBP1-75511AF647 (Bim-AF647.) The product datasheet only says IgG, but no subclass or product number.
A: This item is a mouse IgG1 antibody. For isotype control, I would recommend NBP1-43319AF647 or NBP2-27287AF647.
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Q: Would you recommend a standard Fix/Perm buffer for Bim staining?
A:
Our lab used our standard ICC protocol for cell staining with this product. We used tween-20 in the permeabilization step as BIM can be found in the endomembrane system and mitochondria.