Bim Antibody (SU0318)

Novus Biologicals | Catalog # NBP2-67456

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SU0318 expressed in HEK293
View all Bim Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within human Bim aa 1-40. (SwissProt: O43521 Human; SwissProt: O54918 Mouse)

Localization

Endomembrane system, Mitochondrion.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Bim Antibody (SU0318)

Western Blot: Bim Antibody (SU0318) [NBP2-67456]

Western Blot: Bim Antibody (SU0318) [NBP2-67456]

Western Blot: Bim Antibody (SU0318) [NBP2-67456] - Analysis of Bim on mouse thymus tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.
Immunocytochemistry/ Immunofluorescence: Bim Antibody (SU0318) [NBP2-67456]

Immunocytochemistry/ Immunofluorescence: Bim Antibody (SU0318) [NBP2-67456]

Immunocytochemistry/Immunofluorescence: Bim Antibody (SU0318) [NBP2-67456] - Staining Bim in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: Bim Antibody (SU0318) [NBP2-67456]

Immunohistochemistry-Paraffin: Bim Antibody (SU0318) [NBP2-67456]

Immunohistochemistry-Paraffin: Bim Antibody (SU0318) [NBP2-67456] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Bim antibody. Counter stained with hematoxylin.
Western Blot: Bim Antibody (SU0318) [NBP2-67456]

Western Blot: Bim Antibody (SU0318) [NBP2-67456]

Western Blot: Bim Antibody (SU0318) [NBP2-67456] - Analysis of Bim on different lysates using anti-Bim antibody at 1/1,000 dilution. Positive control: Lane 1: Raji Lane 2: Jurkat Lane 3: A431
Immunocytochemistry/ Immunofluorescence: Bim Antibody (SU0318) [NBP2-67456]

Immunocytochemistry/ Immunofluorescence: Bim Antibody (SU0318) [NBP2-67456]

Immunocytochemistry/Immunofluorescence: Bim Antibody (SU0318) [NBP2-67456] - Staining Bim in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/ Immunofluorescence: Bim Antibody (SU0318) [NBP2-67456]

Immunocytochemistry/ Immunofluorescence: Bim Antibody (SU0318) [NBP2-67456]

Immunocytochemistry/Immunofluorescence: Bim Antibody (SU0318) [NBP2-67456] - Staining Bim in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Applications for Bim Antibody (SU0318)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Bim

Bim, Bcl-2 interacting mediator of cell death, is a pro-apoptotic protein belonging to the

Alternate Names

BAM, bcl-2 interacting mediator of cell death, bcl-2 interacting protein Bim, Bcl2-interacting mediator of cell death, Bcl2-L-11, BCL2-like 11 (apoptosis facilitator), bcl-2-like protein 11, bcl-2-related ovarian death agonist, BIM-alpha6, BIM-beta6, BIM-beta7, BIMBOD, BimEL, BimL

Gene Symbol

BCL2L11

Additional Bim Products

Product Documents for Bim Antibody (SU0318)

Certificate of Analysis

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Product Specific Notices for Bim Antibody (SU0318)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Bim Antibody (SU0318)

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Bim Antibody (SU0318)

Showing  1 - 2 of 2 FAQs Showing All

  • Q: Please recommend which isotype antibody I should use with the NBP1-75511AF647 (Bim-AF647.) The product datasheet only says IgG, but no subclass or product number.

    A: This item is a mouse IgG1 antibody. For isotype control, I would recommend NBP1-43319AF647 or NBP2-27287AF647.

  • Q: Would you recommend a standard Fix/Perm buffer for Bim staining?

    A:

    Our lab used our standard ICC protocol for cell staining with this product. We used tween-20 in the permeabilization step as BIM can be found in the endomembrane system and mitochondria.

  • Q: Please recommend which isotype antibody I should use with the NBP1-75511AF647 (Bim-AF647.) The product datasheet only says IgG, but no subclass or product number.

    A: This item is a mouse IgG1 antibody. For isotype control, I would recommend NBP1-43319AF647 or NBP2-27287AF647.

  • Q: Would you recommend a standard Fix/Perm buffer for Bim staining?

    A:

    Our lab used our standard ICC protocol for cell staining with this product. We used tween-20 in the permeabilization step as BIM can be found in the endomembrane system and mitochondria.

Showing  1 - 2 of 2 FAQs Showing All
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