BLAP75 Antibody

Novus Biologicals | Catalog # NB100-1720

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, Chemotaxis

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all BLAP75 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 275 and 300 of human BLM Associated Protein, 75kDA using the numbering given in entry NP_079221.1 (GeneID 80010).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BLAP75 Antibody

Western Blot: BLAP75 Antibody [NB100-1720]

Western Blot: BLAP75 Antibody [NB100-1720]

Western Blot: BLAP75 Antibody [NB100-1720] - Whole cell lysate (50 ug) from U2OS, HeLa, MCF-7, andLNCaP cells prepared using NETN lysis buffer. Antibody:Affinity purified rabbit anti-BLAP75 antibody used for WB at 0.1 ug/ml. Detection:Chemiluminescence with an exposure time of 3 minutes.
Immunohistochemistry-Paraffin: BLAP75 Antibody [NB100-1720]

Immunohistochemistry-Paraffin: BLAP75 Antibody [NB100-1720]

Immunohistochemistry-Paraffin: BLAP75 Antibody [NB100-1720] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-BLAP75 used at a dilution of 1:200 (1ug/ml). Detection: DAB
Western Blot: BLAP75 Antibody [NB100-1720]

Western Blot: BLAP75 Antibody [NB100-1720]

Western Blot: BLAP75 Antibody [NB100-1720] - Samples: Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-BLAP75 antibody NB100-1720 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate (B).

Applications for BLAP75 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:500

Immunohistochemistry-Paraffin

1:100-1:500

Immunoprecipitation

1-4 ug/mg of lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: BLAP75

BLM-associated polypeptide, 75 kDa (BLAP75) is a component of a dissolvasome complex that contains BLM (Bloom syndrome protein) and TopoIII-alpha and functions to dissolve double Holliday junctions and suppress DNA crossover formation in mitotic cells. BLAP75 is also known as RMI1 homolog, 75 kDa BLM-associated protein, FAAP75, RMI1, C9orf76, FLJ12888, and RP11-346I8.1.

Alternate Names

75 kDa, BLM-associated protein 75 kDa, BLM-associated protein of 75 kDa, chromosome 9 open reading frame 76, FAAP75, FLJ12888, homolog of yeast RecQ-mediated genome instability 1 (RMI1), recQ-mediated genome instability protein 1, RMI1, RecQ mediated genome instability 1, homolog (S. cerevisiae), RMI2

Entrez Gene IDs

80010 (Human)

Gene Symbol

RMI1

UniProt

NP_079221.1 (Human)

Additional BLAP75 Products

Product Documents for BLAP75 Antibody

Certificate of Analysis

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Product Specific Notices for BLAP75 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for BLAP75 Antibody

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Protocols

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