BLIMP1/PRDM1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-17184

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Rat (91%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all BLIMP1/PRDM1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: VGTTLAAPKCNSSTVRFQGLAEGTKGTMKMDMEDADMTLWTEAEFEEKCTYIVNDHPWDSGADGGTSVQAEASLPRNLLFKYATN

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BLIMP1/PRDM1 Antibody - BSA Free

Western Blot: BLIMP1/PRDM1 Antibody [NBP3-17184]

Western Blot: BLIMP1/PRDM1 Antibody [NBP3-17184]

Western Blot: BLIMP1/PRDM1 Antibody [NBP3-17184] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: RT4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: Tonsil
Immunohistochemistry-Paraffin: BLIMP1/PRDM1 Antibody [NBP3-17184]

Immunohistochemistry-Paraffin: BLIMP1/PRDM1 Antibody [NBP3-17184]

Immunohistochemistry-Paraffin: BLIMP1/PRDM1 Antibody [NBP3-17184] - Staining of human tonsil shows cytoplasmic positivity in germinal center and non-germinal center cells.
BLIMP1/PRDM1 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: BLIMP1/PRDM1 Antibody - BSA Free [NBP3-17184]

Chromatin Immunoprecipitation-exo-Seq: BLIMP1/PRDM1 Antibody - BSA Free [NBP3-17184]

ChIP-Exo-Seq composite graph for Anti-PRDM1 (NBP3-17184) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for BLIMP1/PRDM1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BLIMP1/PRDM1

Blimp-1 (B lymphocyte maturation protein-1) is a transcriptional repressor that is required to trigger terminal differentiation of B lymphocytes into Ig secreting cells. Therefore, Blimp 1 is generally considered a "master regulator" for hematopoietic stem cells.

Blimp1 is a nuclear protein with five zinc fingers in its carboxy terminus. The Blimp-1 protein is expressed in, and may be required for terminally differentiated epithelial cells, retinal neurons and other lineage cells. BLIMP-1 has also been implicated in the repression of beta-interferon (IFN-beta) and c-myc gene expression.

Long Name

B Lymphocyte Induced Maturation Protein

Alternate Names

PRDM1, ZNFPR1A1

Gene Symbol

PRDM1

Additional BLIMP1/PRDM1 Products

Product Documents for BLIMP1/PRDM1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for BLIMP1/PRDM1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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