BLOS3 Antibody - BSA Free
Novus Biologicals | Catalog # NBP3-03343
![Immunocytochemistry/ Immunofluorescence: BLOS3 Antibody - BSA Free [NBP3-03343]](https://resources.rndsystems.com/images/products/BLOS3-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-03343-img0004.jpg "Immunocytochemistry/ Immunofluorescence: BLOS3 Antibody - BSA Free [NBP3-03343]")
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Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-202 of human BLOS3 (NP_997715.1). MASQGRRRRPLRRPETVVPGEATETDSERSASSSEEEELYLGPSGPTRGRPTGLRVAGEAAETDSEPEPEPEPTAAPRDLPPLVVQRESAEEAWGTEEAPAPAPARSLLQLRLAESQARLDHDVAAAVSGVYRRAGRDVAALASRLAAAQAAGLAAAHSVRLARGDLCALAERLDIVAGCRLLPDIRGVPGTEPEKDPGPRA
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for BLOS3 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence: BLOS3 Antibody - BSA Free [NBP3-03343]
Immunocytochemistry/Immunofluorescence: BLOS3 Antibody [NBP3-03343] - Immunofluorescence analysis of U-2 OS cells using BLOS3 antibody (NBP3-03343) at dilution of 1:100. Blue: DAPI for nuclear staining.![Immunohistochemistry-Paraffin: BLOS3 Antibody - BSA Free [NBP3-03343]](https://resources.rndsystems.com/images/products/BLOS3 Antibody-Immunohistochemistry-Paraffin-NBP3-03343-img0005.jpg "Immunohistochemistry-Paraffin: BLOS3 Antibody - BSA Free [NBP3-03343]")
Immunohistochemistry-Paraffin: BLOS3 Antibody - BSA Free [NBP3-03343]
Immunohistochemistry-Paraffin: BLOS3 Antibody [NBP3-03343] - Immunohistochemistry of paraffin-embedded mouse testis using BLOS3 antibody (NBP3-03343) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.![Immunocytochemistry/ Immunofluorescence: BLOS3 Antibody - BSA Free [NBP3-03343]](https://resources.rndsystems.com/images/products/BLOS3-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-03343-img0002.jpg "Immunocytochemistry/ Immunofluorescence: BLOS3 Antibody - BSA Free [NBP3-03343]")
Immunocytochemistry/ Immunofluorescence: BLOS3 Antibody - BSA Free [NBP3-03343]
Immunocytochemistry/Immunofluorescence: BLOS3 Antibody [NBP3-03343] - Immunofluorescence analysis of C6 cells using BLOS3 antibody (NBP3-03343) at dilution of 1:100. Blue: DAPI for nuclear staining.![Immunocytochemistry/ Immunofluorescence: BLOS3 Antibody - BSA Free [NBP3-03343]](https://resources.rndsystems.com/images/products/BLOS3-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-03343-img0003.jpg "Immunocytochemistry/ Immunofluorescence: BLOS3 Antibody - BSA Free [NBP3-03343]")
Immunocytochemistry/ Immunofluorescence: BLOS3 Antibody - BSA Free [NBP3-03343]
Immunocytochemistry/Immunofluorescence: BLOS3 Antibody [NBP3-03343] - Immunofluorescence analysis of NIH-3T3 cells using BLOS3 antibody (NBP3-03343) at dilution of 1:100. Blue: DAPI for nuclear staining.![Immunohistochemistry-Paraffin: BLOS3 Antibody - BSA Free [NBP3-03343]](https://resources.rndsystems.com/images/products/BLOS3-Antibody-Immunohistochemistry-Paraffin-NBP3-03343-img0001.jpg "Immunohistochemistry-Paraffin: BLOS3 Antibody - BSA Free [NBP3-03343]")
Immunohistochemistry-Paraffin: BLOS3 Antibody - BSA Free [NBP3-03343]
Immunohistochemistry-Paraffin: BLOS3 Antibody [NBP3-03343] - Paraffin-embedded human colon using BLOS3 antibody at dilution of 1:100 (40x lens).Applications for BLOS3 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry
1:50-1:200
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: BLOS3
Alternate Names
biogenesis of lysosomal organelles complex-1, subunit 3, biogenesis of lysosome-related organelles complex 1 subunit 3, BLOS3, HPS8, RP
Gene Symbol
BLOC1S3
Additional BLOS3 Products
Product Documents for BLOS3 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for BLOS3 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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