BLZF1 Antibody

Novus Biologicals | Catalog # NBP2-15596

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Primate (99%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all BLZF1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human BLZF1. The exact sequence is proprietary.

Reactivity Notes

Chimpanzee (99%), Rat (84%), Bovine (87%).

Localization

Golgi apparatus lumen; Isoform 1: Nucleus; Isoform 2: Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for BLZF1 Antibody

Western Blot: BLZF1 Antibody [NBP2-15596]

Western Blot: BLZF1 Antibody [NBP2-15596]

Western Blot: BLZF1 Antibody [NBP2-15596] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with BLZF1 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: BLZF1 Antibody [NBP2-15596]

Immunocytochemistry/ Immunofluorescence: BLZF1 Antibody [NBP2-15596]

Immunocytochemistry/Immunofluorescence: BLZF1 Antibody [NBP2-15596] - BLZF1 antibody detects BLZF1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: BLZF1 protein stained by BLZF1 antibody diluted at 1:500. Blue: Hoechst 33343 staining.
Western Blot: BLZF1 Antibody [NBP2-15596]

Western Blot: BLZF1 Antibody [NBP2-15596]

Western Blot: BLZF1 Antibody [NBP2-15596] - Sample (30 ug of whole cell lysate) A: NIH-3T3 10% SDS PAGE gel, diluted at 1:1000.
Western Blot: BLZF1 Antibody [NBP2-15596]

Western Blot: BLZF1 Antibody [NBP2-15596]

Western Blot: BLZF1 Antibody [NBP2-15596] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with BLZF1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Applications for BLZF1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:10000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: BLZF1

Required for normal Golgi structure and for protein transport from the endoplasmic reticulum (ER) through the Golgi apparatus to the cell surface.

Alternate Names

basic leucine zipper nuclear factor 1JEM-1s, cytoplasmic protein, GOLGIN-45, JEM1, JEM-1MGC22497, JEM-1short protein, p45 basic leucine-zipper nuclear factor

Gene Symbol

BLZF1

UniProt

Q9H2G9 (Human)

Additional BLZF1 Products

Product Documents for BLZF1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for BLZF1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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