BMP-2 Quantikine ELISA Kit

  (32 citations)
(6 Reviews)
    
Datasheet / CoA / SDS
Product Details
Assay Procedure
Citations (32)
FAQs
Supplemental Products
Reviews
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Bone Extracts (50 uL)
  • Sensitivity
    29 pg/mL
  • Assay Range
    62.5 - 4,000 pg/mL (Cell Culture Supernates, Bone Extracts)
  • Specificity
    Natural and mammalian-expressed recombinant human BMP-2.  This assay does not recognize E. coli-expressed BMP-2.
  • Cross-reactivity
    Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    Interference observed with 1 or more available related molecules.
Control Available
QC49 , Quantikine Immunoassay Control Set 973 for H/M/R BMP-2 - Please Inquire
Product Summary
The Quantikine Human BMP-2 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human BMP-2 in bone tissue extracts and cell culture supernates. It contains CHO cell-expressed recombinant human BMP-2 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse BMP-2 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human BMP-2.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Bone Extracts
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 323 1010 2313 344 1043 2213
Standard Deviation 8.3 28.3 56 25.2 55.4 140.2
CV% 2.6 2.8 2.4 7.3 5.3 6.3

Recovery

The recovery of recombinant human BMP-2 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
2 M Guanidine-HCI (n=1) 98 94-101
2 M Urea (n=1) 101 92-106
Cell Culture Media (n=4) 102 94-110
Linearity
The following samples were spiked with rhBMP-2 and then diluted with Calibrator Diluent. The samples were then serially diluted and tested to assess the linearity of the assay.
 BMP-2 [HRP]
Product Datasheets

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Certificate of Analysis Lookup

Certificate of Analysis Request Form

To download a Certificate of Analysis, please enter the catalog and lot numbers in the search box below.

Note: Certificate of Analysis not available for kit components.

Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: BMP-2

BMPs are secreted signaling molecules that comprise a subfamily of the TGF-beta superfamily and were originally identified as regulators of cartilage and bone formation. There are at least 20 structurally and functionally related BMPs, most of which play roles in embryogenesis and morphogenesis of various tissues and organs. Biologically active BMPs are usually homodimers containing a characteristic cysteine knot structure. Heterodimers, BMP-2/BMP-7 and BMP-4/BMP-7 have also been suggested to exist and function in vivo. They are more potent inducers of bone formation than their respective homodimers. In addition, heterodimers, but not homodimers, are ventral mesoderm inducers. Heterodimer activity may be mediated by a different or additional receptor subtype.

Decapentaplegic (Dpp) is one of at least five TGF-beta superfamily ligands identified in the Drosophila genome. Dpp, a functional ortholog of mammalian BMP-2 and BMP-4, is a morphogen and plays an essential role in Drosophila development. Dpp regulates embryonic dorsal-ventral polarity and is required for gut morphogenesis and outgrowth and patterning of imaginal disks.

  • Long Name:
    Bone Morphogenetic Protein 2
  • Entrez Gene IDs:
    650 (Human); 12156 (Mouse); 29373 (Rat)
  • Alternate Names:
    BMP2; BMP-2; BMP-2A; BMP2ABone morphogenetic protein 2A; bone morphogenetic protein 2
Related Research Areas
Assay Procedure
Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

32 Citations: Showing 1 - 10
Filter your results:

Species
Sample Type
  1. Inflammatory cell response to ultra-thin amorphous and crystalline hydroxyapatite surfaces
    Authors: Louise Rydén
    J Mater Sci Mater Med, 2017;28(1):9.
    Species: Human
    Sample Type: Cell Culture Supernates
  2. Inhibiting PHD2 in bone marrow mesenchymal stem cells via lentiviral vector-mediated RNA interference facilitates the repair of periodontal tissue defects in SD rats
    Authors: C Chen, H Li, J Jiang, Q Zhang, F Yan
    Oncotarget, 2017;8(42):72676-72699.
    Species: Rat
    Sample Type: Cell Culture Supernates
  3. BMP2-modified injectable hydrogel for osteogenic differentiation of human periodontal ligament stem cells
    Authors: SH Park, JS Kwon, BS Lee, JH Park, BK Lee, JH Yun, BY Lee, JH Kim, BH Min, TH Yoo, MS Kim
    Sci Rep, 2017;7(1):6603.
    Species: Human
    Sample Type: Protein
  4. Differential effects of amnion and chorion membrane extracts on osteoblast-like cells due to the different growth factor composition of the extracts
    Authors: YY Go, SE Kim, GJ Cho, SW Chae, JJ Song
    PLoS ONE, 2017;12(8):e0182716.
    Species: Human
    Sample Type: Tissue Homogenates
  5. Bone morphogenetic protein-2 loaded poly(D,L-lactide-co-glycolide) microspheres enhance osteogenic potential of gelatin/hydroxyapatite/?-tricalcium phosphate cryogel composite for alveolar ridge augmentation
    Authors: HC Chang, C Yang, F Feng, FH Lin, CH Wang, PC Chang
    J. Formos. Med. Assoc, 2017;0(0):.
    Species: Human
    Sample Type: Recombinant Protein
  6. Modified mRNA for BMP 2 in combination with biomaterials serves as a transcript activated matrix for effectively inducing osteogenic pathways in stem cells
    Stem Cells Dev, 2016;0(0):.
    Species: Rat
    Sample Type: Cell Culture Supernates
  7. Facilitated receptor-recognition and enhanced bioactivity of bone morphogenetic protein-2 on magnesium-substituted hydroxyapatite surface
    Authors: B Huang, Y Yuan, T Li, S Ding, W Zhang, Y Gu, C Liu
    Sci Rep, 2016;6(0):24323.
    Species: Mouse
    Sample Type: Cell Culture Supernates
  8. Targeted next-generation sequencing reveals multiple deleterious variants in OPLL-associated genes
    Authors: Xin Chen
    Sci Rep, 2016;6(0):26962.
    Species: Human
    Sample Type: Plasma
  9. Increased plasma BMP-2 levels are associated with atherosclerosis burden and coronary calcification in type 2 diabetic patients.
    Authors: Zhang M, Sara J, Wang F, Liu L, Su L, Zhe J, Wu X, Liu J
    Cardiovasc Diabetol, 2015;14(0):64.
    Species: Human
    Sample Type: Plasma
  10. Disequilibrium of BMP2 levels in the breast stem cell niche launches epithelial transformation by overamplifying BMPR1B cell response.
    Authors: Chapellier M, Bachelard-Cascales E, Schmidt X, Clement F, Treilleux I, Delay E, Jammot A, Menetrier-Caux C, Pochon G, Besancon R, Voeltzel T, Caron de Fromentel C, Caux C, Blay J, Iggo R, Maguer-Satta V
    Stem Cell Reports, 2015;4(2):239-54.
    Species: Human
    Sample Type: Tissue Homogenates
  11. The role of transduced bone marrow cells overexpressing BMP-2 in healing critical-sized defects in a mouse femur.
    Authors: Pensak M, Hong S, Dukas A, Tinsley B, Drissi H, Tang A, Cote M, Sugiyama O, Lichtler A, Rowe D, Lieberman J
    Gene Ther, 2015;22(6):467-75.
    Species: Mouse
    Sample Type: Cell Culture Supernates
  12. Dental follicle stem cells in bone regeneration on titanium implants.
    Authors: Lucaciu O, Soritau O, Gheban D, Ciuca D, Virtic O, Vulpoi A, Dirzu N, Campian R, Baciut G, Popa C, Simon S, Berce P, Baciut M, Crisan B
    BMC Biotechnol, 2015;15(1):114.
    Species: Human
    Sample Type: Cell Culture Supernates
  13. Identifying an ovarian cancer cell hierarchy regulated by bone morphogenetic protein 2.
    Authors: Choi Y, Ingram P, Yang K, Coffman L, Iyengar M, Bai S, Thomas D, Yoon E, Buckanovich R
    Proc Natl Acad Sci U S A, 2015;112(50):E6882-8.
    Species: Human
    Sample Type: Cell Culture Supernates
  14. Simple signaling molecules for inductive bone regenerative engineering.
    Authors: Cushnie, Emily K, Ulery, Bret D, Nelson, Stephen, Deng, Meng, Sethuraman, Swaminat, Doty, Stephen, Lo, Kevin W, Khan, Yusuf M, Laurencin, Cato T
    PLoS ONE, 2014;9(7):e101627.
    Species: Human
    Sample Type: Cell Culture Supernates
  15. Neuroprotection by rAAV-mediated gene transfer of bone morphogenic protein 7.
    Authors: Heinonen A, Rahman M, Dogbevia G, Jakobi H, Wolfl S, Sprengel R, Schwaninger M
    BMC Neurosci, 2014;15(0):38.
    Species: Mouse
    Sample Type: Cell Culture Supernates
  16. Asparagine-linked glycosylation of bone morphogenetic protein-2 is required for secretion and osteoblast differentiation.
    Authors: Hang, Qinglei, Zhou, Ying, Hou, Sicong, Zhang, Dongmei, Yang, Xiaojing, Chen, Jianping, Ben, Zhiyun, Cheng, Chun, Shen, Aiguo
    Glycobiology, 2014;24(3):292-304.
    Species: Hamster
    Sample Type: Cell Lysates
  17. Designing nanotopographical density of extracellular matrix for controlled morphology and function of human mesenchymal stem cells.
    Authors: Kim J, Kim H, Lim K, Kim Y, Seonwoo H, Park S, Lim H, Kim D, Suh K, Choung P, Choung Y, Chung J
    Sci Rep, 2013;3(0):3552.
    Species: Human
    Sample Type: Cell Culture Supernates
  18. Bone morphogenetic protein-2/-4 upregulation promoted by endothelial cells in coculture enhances mouse embryoid body differentiation.
    Authors: Talavera-Adame, Dodanim, Gupta, Ankur, Kurtovic, Silvia, Chaiboonma, Kira L, Arumugaswami, Vaithili, Dafoe, Donald C
    Stem Cells Dev, 2013;22(24):3252-60.
    Species: Human
    Sample Type: Cell Culture Supernates
  19. The serum bone morphogenetic protein-2 level in non-small-cell lung cancer patients.
    Authors: Choi YJ, Kim ST, Park KH, Oh SC, Seo JH, Shin SW, Kim JS, Kim YH
    Med. Oncol., 2012;29(0):582-8.
    Species: Human
    Sample Type: Serum
  20. Association of bone morphogenetic proteins with spinal fusion in ankylosing spondylitis.
    Authors: Chen HA, Chen CH, Lin YJ, Chen PC, Chen WS, Lu CL, Chou CT
    J. Rheumatol., 2010;37(10):2126-32.
    Species: Human
    Sample Type: Serum
  21. Bone morphogenetic protein-2 may represent the molecular link between oxidative stress and vascular stiffness in chronic kidney disease.
    Authors: Dalfino G, Simone S, Porreca S, Cosola C, Balestra C, Manno C, Schena FP, Grandaliano G, Pertosa G
    Atherosclerosis, 2010;211(2):418-23.
    Species: Human
    Sample Type: Serum
  22. Periodontal regeneration using ex vivo autologous stem cells engineered to express the BMP-2 gene: an alternative to alveolaplasty.
    Authors: Chen YL, Chen PK, Jeng LB, Huang CS, Yang LC, Chung HY, Chang SC
    Gene Ther., 2008;15(22):1469-77.
    Species: Rabbit
    Sample Type: Cell Culture Supernates
  23. Isolation and characterization of CD146+ multipotent mesenchymal stromal cells.
    Authors: Sorrentino A, Ferracin M, Castelli G, Biffoni M, Tomaselli G, Baiocchi M, Fatica A, Negrini M, Peschle C, Valtieri M
    Exp. Hematol., 2008;36(8):1035-46.
    Species: Human
    Sample Type: Cell Culture Supernates
  24. Bone marrow cells are a rich source of growth factors and cytokines: implications for cell therapy trials after myocardial infarction.
    Authors: Korf-Klingebiel M, Kempf T, Sauer T, Brinkmann E, Fischer P, Meyer GP, Ganser A, Drexler H, Wollert KC
    Eur. Heart J., 2008;29(23):2851-8.
    Species: Human
    Sample Type: Cell Culture Supernates
  25. Mesenchymal stem cells support migration, extracellular matrix invasion, proliferation, and survival of endothelial cells in vitro.
    Authors: Potapova IA, Gaudette GR, Brink PR, Robinson RB, Rosen MR, Cohen IS, Doronin SV
    Stem Cells, 2007;25(7):1761-8.
    Species: Human
    Sample Type: Cell Culture Supernates
  26. Activin-like kinase receptor 1 (ALK1) in atherosclerotic lesions and vascular mesenchymal cells.
    Authors: Yao Y, Zebboudj AF, Torres A, Shao E, Bostrom K
    Cardiovasc. Res., 2007;74(2):279-89.
    Species: Human
    Sample Type: Cell Culture Supernates
  27. Quantitative assessment of growth factors in reaming aspirate, iliac crest, and platelet preparation.
    Authors: Herrmann S, Green J, Weber T, Scharfenberger A
    Bone, 2006;39(5):1156-63.
    Species: Human
    Sample Type: Tissue Homogenates
  28. Targeted delivery system for juxtacrine signaling growth factor based on rhBMP-2-mediated carrier-protein conjugation.
    Authors: Liu HW, Chen CH, Tsai CL, Hsiue GH
    Bone, 2006;39(4):825-36.
    Species: Human
    Sample Type: Recombinant Protein
  29. Direct adenoviral transfer of bone morphogenetic protein-2 cDNA enhances fracture healing in osteoporotic sheep.
    Authors: Egermann M, Baltzer AW, Adamaszek S, Evans C, Robbins P, Schneider E, Lill CA
    Hum. Gene Ther., 2006;17(5):507-17.
    Species: Ovine
    Sample Type: Cell Culture Supernates
  30. Combinatorial gene therapy for bone regeneration: cooperative interactions between adenovirus vectors expressing bone morphogenetic proteins 2, 4, and 7.
    Authors: Zhao M, Zhao Z, Koh JT, Jin T, Franceschi RT
    J. Cell. Biochem., 2005;95(1):1-16.
    Species: Mouse
    Sample Type: Cell Culture Supernates
  31. Measurement of bone morphogenetic proteins and other growth factors in demineralized bone matrix.
    Authors: Blum B, Moseley J, Miller L, Richelsoph K, Haggard W
    Orthopedics, 2004;27(1):161-5.
    Species: Rat
    Sample Type: Tissue Homogenates
  32. Shock wave application enhances pertussis toxin protein-sensitive bone formation of segmental femoral defect in rats.
    Authors: Chen YJ, Kuo YR, Yang KD, Wang CJ, Huang HC, Wang FS
    J. Bone Miner. Res., 2003;18(12):2169-79.
    Species: Rat
    Sample Type: Serum
Expand to show all Citations

FAQs

  1. Does the BMP-2 Quantikine® ELISA kit (Catalog # DBP200) recognize all forms of BMP-2? Does this kit detect the BMP-2/BMP-7 heterodimer?

     

    • The BMP-2 Quantikine® ELISA kit (Catalog # DBP200) is specific to mammalian expressed BMP-2. Recombinant E.coli expressed BMP-2 (Catalog # 355-BEC) is not detected in this assay. Cross-reactivity information is provided under the specificity section of the kit booklet. This kit does not detect the BMP-2/BMP-7 heterodimer.

View all ELISA FAQs
ELISA Controls
Description Application Cat# Citations Images  

Quantikine Immunoassay Control Set 973 for H/M/R BMP-2

Ctrl QC49
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Supplemental ELISA Products
Description Application Cat# Citations Images  

Quantikine ELISA Wash Buffer 1

ELISA WA126 6  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Average Rating: 4.7 (Based on 6 reviews)

Have you used BMP-2 Quantikine ELISA Kit?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Read what people are saying who have used BMP-2 Quantikine ELISA Kit.

Filter by Ratings
Excellent
(4)
Very Good
(2)
All Ratings
Filter by Applications
All Applications
Filter by Species
All Species

Images Ratings Applications Species Reviewed By Date Details
 BMP-2 Quantikine ELISA Kit DBP200 Array DBP200
Enlarge
Excellent
  Anonymous 08/24/2017
Show
Image Details
 BMP-2 Quantikine ELISA Kit DBP200
: BMP-2 Quantikine ELISA Kit [DBP200].

Summary

Sample Tested scaffolds loaded with BMP2
 BMP-2 Quantikine ELISA Kit DBP200 Array DBP200
Enlarge
Excellent
  Anonymous 05/12/2016
Show
Image Details
 BMP-2 Quantikine ELISA Kit DBP200
: BMP-2 Quantikine ELISA Kit [DBP200].

Summary

Sample Tested Bone Extracts
 BMP-2 Quantikine ELISA Kit DBP200 Array DBP200
Enlarge
Excellent
  Anonymous 05/09/2016
Show
Image Details
 BMP-2 Quantikine ELISA Kit DBP200
: BMP-2 Quantikine ELISA Kit [DBP200].

Summary

Sample Tested Cartilage tissue
 BMP-2 Quantikine ELISA Kit DBP200 Array DBP200
Enlarge
Very Good
  Anonymous 05/05/2016
Show
Image Details
 BMP-2 Quantikine ELISA Kit DBP200
: BMP-2 Quantikine ELISA Kit [DBP200].

Summary

Sample Tested Bone Extracts
  Excellent
  Anonymous 05/05/2016
Show

Summary

Sample Tested Human tissue
 BMP-2 Quantikine ELISA Kit DBP200 Array DBP200
Enlarge
Very Good
  Anonymous 05/04/2016
Show
Image Details
 BMP-2 Quantikine ELISA Kit DBP200
: BMP-2 Quantikine ELISA Kit [DBP200].

Summary

Sample Tested Bone Extracts

Please scroll the above reviews for more

Order Details

Contact Us to Order
reload

Feedback

Customer Information
R&D Systems Guarantee