BMP-2 Quantikine ELISA Kit

(30 citations)
(6 Reviews)
  
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Bone Extracts (50 uL)
  • Sensitivity
    29 pg/mL
  • Assay Range
    62.5 - 4,000 pg/mL (Cell Culture Supernates, Bone Extracts)
  • Specificity
    Natural and mammalian-expressed recombinant human BMP-2.  This assay does not recognize E. coli-expressed BMP-2.
  • Cross-reactivity
    Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    Interference observed with 1 or more available related molecules.
Control Available
QC49, Quantikine Immunoassay Control Set 830 for H/M/R BMP-2 - Please Inquire
Product Summary
The Quantikine Human BMP-2 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human BMP-2 in bone tissue extracts and cell culture supernates. It contains CHO cell-expressed recombinant human BMP-2 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse BMP-2 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human BMP-2.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Bone Extracts
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020404040
Mean3231010231334410432213
Standard Deviation8.328.35625.255.4140.2
CV%2.62.82.47.35.36.3

Recovery

The recovery of recombinant human BMP-2 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
2 M Guanidine-HCI (n=1) 98 94-101
2 M Urea (n=1) 101 92-106
Cell Culture Media (n=4) 102 94-110
Linearity
The following samples were spiked with rhBMP-2 and then diluted with Calibrator Diluent. The samples were then serially diluted and tested to assess the linearity of the assay.
BMP-2 Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: BMP-2

BMPs are secreted signaling molecules that comprise a subfamily of the TGF-beta superfamily and were originally identified as regulators of cartilage and bone formation. There are at least 20 structurally and functionally related BMPs, most of which play roles in embryogenesis and morphogenesis of various tissues and organs. Biologically active BMPs are usually homodimers containing a characteristic cysteine knot structure. Heterodimers, BMP-2/BMP-7 and BMP-4/BMP-7 have also been suggested to exist and function in vivo. They are more potent inducers of bone formation than their respective homodimers. In addition, heterodimers, but not homodimers, are ventral mesoderm inducers. Heterodimer activity may be mediated by a different or additional receptor subtype.

Decapentaplegic (Dpp) is one of at least five TGF-beta superfamily ligands identified in the Drosophila genome. Dpp, a functional ortholog of mammalian BMP-2 and BMP-4, is a morphogen and plays an essential role in Drosophila development. Dpp regulates embryonic dorsal-ventral polarity and is required for gut morphogenesis and outgrowth and patterning of imaginal disks.

    • Long Name
      Bone Morphogenetic Protein 2
    • Entrez Gene IDs
      650 (Human); 12156 (Mouse); 29373 (Rat);
    • Alternate Names
      BMP2; BMP-2; BMP-2A; BMP2ABone morphogenetic protein 2A; bone morphogenetic protein 2;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 100 µL Assay Diluent
    4.   Add 100 µL of Assay Diluent to each well.

    5. 50 µL Standard, Control, or Sample
    6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
    7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

    8. 200 µL Conjugate
    9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
    10.   Aspirate and wash 4 times.

    11. 200 µL Substrate Solution
    12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

    13. 50 µL Stop Solution
    14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    30 Citations: Showing 1 - 10
    Filter your results:

    Species
    Sample Type
    1. BMP2-modified injectable hydrogel for osteogenic differentiation of human periodontal ligament stem cells
      Authors: SH Park, JS Kwon, BS Lee, JH Park, BK Lee, JH Yun, BY Lee, JH Kim, BH Min, TH Yoo, MS Kim
      Sci Rep, 2017;7(1):6603.
      Species: Human
      Sample Type: Protein
    2. Differential effects of amnion and chorion membrane extracts on osteoblast-like cells due to the different growth factor composition of the extracts
      Authors: YY Go, SE Kim, GJ Cho, SW Chae, JJ Song
      PLoS ONE, 2017;12(8):e0182716.
      Species: Human
      Sample Type: Tissue Homogenates
    3. Bone morphogenetic protein-2 loaded poly(D,L-lactide-co-glycolide) microspheres enhance osteogenic potential of gelatin/hydroxyapatite/?-tricalcium phosphate cryogel composite for alveolar ridge augmentation
      Authors: HC Chang, C Yang, F Feng, FH Lin, CH Wang, PC Chang
      J. Formos. Med. Assoc, 2017;0(0):.
      Species: Human
      Sample Type: Recombinant Protein
    4. Modified mRNA for BMP 2 in combination with biomaterials serves as a transcript activated matrix for effectively inducing osteogenic pathways in stem cells
      Stem Cells Dev, 2016;0(0):.
      Species: Rat
      Sample Type: Cell Culture Supernates
    5. Facilitated receptor-recognition and enhanced bioactivity of bone morphogenetic protein-2 on magnesium-substituted hydroxyapatite surface
      Authors: B Huang, Y Yuan, T Li, S Ding, W Zhang, Y Gu, C Liu
      Sci Rep, 2016;6(0):24323.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    6. Targeted next-generation sequencing reveals multiple deleterious variants in OPLL-associated genes
      Authors: Xin Chen
      Sci Rep, 2016;6(0):26962.
      Species: Human
      Sample Type: Plasma
    7. Identifying an ovarian cancer cell hierarchy regulated by bone morphogenetic protein 2.
      Authors: Choi Y, Ingram P, Yang K, Coffman L, Iyengar M, Bai S, Thomas D, Yoon E, Buckanovich R
      Proc Natl Acad Sci U S A, 2015;112(50):E6882-8.
      Species: Human
      Sample Type: Cell Culture Supernates
    8. Disequilibrium of BMP2 levels in the breast stem cell niche launches epithelial transformation by overamplifying BMPR1B cell response.
      Authors: Chapellier M, Bachelard-Cascales E, Schmidt X, Clement F, Treilleux I, Delay E, Jammot A, Menetrier-Caux C, Pochon G, Besancon R, Voeltzel T, Caron de Fromentel C, Caux C, Blay J, Iggo R, Maguer-Satta V
      Stem Cell Reports, 2015;4(2):239-54.
      Species: Human
      Sample Type: Tissue Homogenates
    9. The role of transduced bone marrow cells overexpressing BMP-2 in healing critical-sized defects in a mouse femur.
      Authors: Pensak M, Hong S, Dukas A, Tinsley B, Drissi H, Tang A, Cote M, Sugiyama O, Lichtler A, Rowe D, Lieberman J
      Gene Ther, 2015;22(6):467-75.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    10. Dental follicle stem cells in bone regeneration on titanium implants.
      Authors: Lucaciu O, Soritau O, Gheban D, Ciuca D, Virtic O, Vulpoi A, Dirzu N, Campian R, Baciut G, Popa C, Simon S, Berce P, Baciut M, Crisan B
      BMC Biotechnol, 2015;15(1):114.
      Species: Human
      Sample Type: Cell Culture Supernates
    11. Increased plasma BMP-2 levels are associated with atherosclerosis burden and coronary calcification in type 2 diabetic patients.
      Authors: Zhang M, Sara J, Wang F, Liu L, Su L, Zhe J, Wu X, Liu J
      Cardiovasc Diabetol, 2015;14(0):64.
      Species: Human
      Sample Type: Plasma
    12. Asparagine-linked glycosylation of bone morphogenetic protein-2 is required for secretion and osteoblast differentiation.
      Authors: Hang, Qinglei, Zhou, Ying, Hou, Sicong, Zhang, Dongmei, Yang, Xiaojing, Chen, Jianping, Ben, Zhiyun, Cheng, Chun, Shen, Aiguo
      Glycobiology, 2014;24(3):292-304.
      Species: Hamster
      Sample Type: Cell Lysates
    13. Neuroprotection by rAAV-mediated gene transfer of bone morphogenic protein 7.
      Authors: Heinonen A, Rahman M, Dogbevia G, Jakobi H, Wolfl S, Sprengel R, Schwaninger M
      BMC Neurosci, 2014;15(0):38.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    14. Simple signaling molecules for inductive bone regenerative engineering.
      Authors: Cushnie, Emily K, Ulery, Bret D, Nelson, Stephen, Deng, Meng, Sethuraman, Swaminat, Doty, Stephen, Lo, Kevin W, Khan, Yusuf M, Laurencin, Cato T
      PLoS ONE, 2014;9(7):e101627.
      Species: Human
      Sample Type: Cell Culture Supernates
    15. Designing nanotopographical density of extracellular matrix for controlled morphology and function of human mesenchymal stem cells.
      Authors: Kim J, Kim H, Lim K, Kim Y, Seonwoo H, Park S, Lim H, Kim D, Suh K, Choung P, Choung Y, Chung J
      Sci Rep, 2013;3(0):3552.
      Species: Human
      Sample Type: Cell Culture Supernates
    16. Bone morphogenetic protein-2/-4 upregulation promoted by endothelial cells in coculture enhances mouse embryoid body differentiation.
      Authors: Talavera-Adame, Dodanim, Gupta, Ankur, Kurtovic, Silvia, Chaiboonma, Kira L, Arumugaswami, Vaithili, Dafoe, Donald C
      Stem Cells Dev, 2013;22(24):3252-60.
      Species: Human
      Sample Type: Cell Culture Supernates
    17. The serum bone morphogenetic protein-2 level in non-small-cell lung cancer patients.
      Authors: Choi YJ, Kim ST, Park KH, Oh SC, Seo JH, Shin SW, Kim JS, Kim YH
      Med. Oncol., 2012;29(0):582-8.
      Species: Human
      Sample Type: Serum
    18. Association of bone morphogenetic proteins with spinal fusion in ankylosing spondylitis.
      Authors: Chen HA, Chen CH, Lin YJ, Chen PC, Chen WS, Lu CL, Chou CT
      J. Rheumatol., 2010;37(10):2126-32.
      Species: Human
      Sample Type: Serum
    19. Bone morphogenetic protein-2 may represent the molecular link between oxidative stress and vascular stiffness in chronic kidney disease.
      Authors: Dalfino G, Simone S, Porreca S, Cosola C, Balestra C, Manno C, Schena FP, Grandaliano G, Pertosa G
      Atherosclerosis, 2010;211(2):418-23.
      Species: Human
      Sample Type: Serum
    20. Bone marrow cells are a rich source of growth factors and cytokines: implications for cell therapy trials after myocardial infarction.
      Authors: Korf-Klingebiel M, Kempf T, Sauer T, Brinkmann E, Fischer P, Meyer GP, Ganser A, Drexler H, Wollert KC
      Eur. Heart J., 2008;29(23):2851-8.
      Species: Human
      Sample Type: Cell Culture Supernates
    21. Periodontal regeneration using ex vivo autologous stem cells engineered to express the BMP-2 gene: an alternative to alveolaplasty.
      Authors: Chen YL, Chen PK, Jeng LB, Huang CS, Yang LC, Chung HY, Chang SC
      Gene Ther., 2008;15(22):1469-77.
      Species: Rabbit
      Sample Type: Cell Culture Supernates
    22. Isolation and characterization of CD146+ multipotent mesenchymal stromal cells.
      Authors: Sorrentino A, Ferracin M, Castelli G, Biffoni M, Tomaselli G, Baiocchi M, Fatica A, Negrini M, Peschle C, Valtieri M
      Exp. Hematol., 2008;36(8):1035-46.
      Species: Human
      Sample Type: Cell Culture Supernates
    23. Mesenchymal stem cells support migration, extracellular matrix invasion, proliferation, and survival of endothelial cells in vitro.
      Authors: Potapova IA, Gaudette GR, Brink PR, Robinson RB, Rosen MR, Cohen IS, Doronin SV
      Stem Cells, 2007;25(7):1761-8.
      Species: Human
      Sample Type: Cell Culture Supernates
    24. Activin-like kinase receptor 1 (ALK1) in atherosclerotic lesions and vascular mesenchymal cells.
      Authors: Yao Y, Zebboudj AF, Torres A, Shao E, Bostrom K
      Cardiovasc. Res., 2007;74(2):279-89.
      Species: Human
      Sample Type: Cell Culture Supernates
    25. Quantitative assessment of growth factors in reaming aspirate, iliac crest, and platelet preparation.
      Authors: Herrmann S, Green J, Weber T, Scharfenberger A
      Bone, 2006;39(5):1156-63.
      Species: Human
      Sample Type: Tissue Homogenates
    26. Targeted delivery system for juxtacrine signaling growth factor based on rhBMP-2-mediated carrier-protein conjugation.
      Authors: Liu HW, Chen CH, Tsai CL, Hsiue GH
      Bone, 2006;39(4):825-36.
      Species: Human
      Sample Type: Recombinant Protein
    27. Direct adenoviral transfer of bone morphogenetic protein-2 cDNA enhances fracture healing in osteoporotic sheep.
      Authors: Egermann M, Baltzer AW, Adamaszek S, Evans C, Robbins P, Schneider E, Lill CA
      Hum. Gene Ther., 2006;17(5):507-17.
      Species: Ovine
      Sample Type: Cell Culture Supernates
    28. Combinatorial gene therapy for bone regeneration: cooperative interactions between adenovirus vectors expressing bone morphogenetic proteins 2, 4, and 7.
      Authors: Zhao M, Zhao Z, Koh JT, Jin T, Franceschi RT
      J. Cell. Biochem., 2005;95(1):1-16.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    29. Measurement of bone morphogenetic proteins and other growth factors in demineralized bone matrix.
      Authors: Blum B, Moseley J, Miller L, Richelsoph K, Haggard W
      Orthopedics, 2004;27(1):161-5.
      Species: Rat
      Sample Type: Tissue Homogenates
    30. Shock wave application enhances pertussis toxin protein-sensitive bone formation of segmental femoral defect in rats.
      Authors: Chen YJ, Kuo YR, Yang KD, Wang CJ, Huang HC, Wang FS
      J. Bone Miner. Res., 2003;18(12):2169-79.
      Species: Rat
      Sample Type: Serum
    Expand to show all 30 Citations
    ELISA Controls
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    Quantikine Immunoassay Control Set 830 for H/M/R BMP-2

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    Average Rating: 4.7 (Based on 6 reviews)

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     BMP-2 Quantikine ELISA Kit DBP200
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    Excellent
      Anonymous 08/24/2017
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     BMP-2 Quantikine ELISA Kit DBP200
    : BMP-2 Quantikine ELISA Kit [DBP200]

    Summary

    Sample Testedscaffolds loaded with BMP2
     BMP-2 Quantikine ELISA Kit DBP200
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    Excellent
      Anonymous 05/12/2016
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     BMP-2 Quantikine ELISA Kit DBP200
    : BMP-2 Quantikine ELISA Kit [DBP200]

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    Sample TestedBone Extracts
     BMP-2 Quantikine ELISA Kit DBP200
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    Excellent
      Anonymous 05/09/2016
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     BMP-2 Quantikine ELISA Kit DBP200
    : BMP-2 Quantikine ELISA Kit [DBP200]

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    Sample TestedCartilage tissue
      Very Good
      Anonymous 05/05/2016
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    Sample TestedBone Extracts
      Excellent
      Anonymous 05/05/2016
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    Sample TestedHuman tissue
     BMP-2 Quantikine ELISA Kit DBP200
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    Very Good
      Anonymous 05/04/2016
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     BMP-2 Quantikine ELISA Kit DBP200
    : BMP-2 Quantikine ELISA Kit [DBP200]

    Summary

    Sample TestedBone Extracts

    FAQs

    1. Does the BMP-2 Quantikine® ELISA kit (Catalog # DBP200) recognize all forms of BMP-2? Does this kit detect the BMP-2/BMP-7 heterodimer?

       

      • The BMP-2 Quantikine® ELISA kit (Catalog # DBP200) is specific to mammalian expressed BMP-2. Recombinant E.coli expressed BMP-2 (Catalog # 355-BEC) is not detected in this assay. Cross-reactivity information is provided under the specificity section of the kit booklet. This kit does not detect the BMP-2/BMP-7 heterodimer.

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