BMP-2 Quantikine ELISA Kit

Catalog # Availability Size / Price Qty
PDBP200
SBP200
DBP200
Control Products Available
Human/Mouse/Rat BMP-2 ELISA Standard Curve
2 Images
Product Details
Procedure
Citations (40)
FAQs
Supplemental Products
Reviews (7)

BMP-2 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Bone Extracts (50 uL)
Sensitivity
29 pg/mL
Assay Range
62.5 - 4,000 pg/mL (Cell Culture Supernates, Bone Extracts)
Specificity
Natural and mammalian-expressed recombinant human BMP-2.  This assay does not recognize E. coli-expressed BMP-2.
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.

Product Summary

The Quantikine Human BMP-2 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human BMP-2 in bone tissue extracts and cell culture supernates. It contains CHO cell-expressed recombinant human BMP-2 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse BMP-2 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human BMP-2.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Bone Extracts

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 323 1010 2313 344 1043 2213
Standard Deviation 8.3 28.3 56 25.2 55.4 140.2
CV% 2.6 2.8 2.4 7.3 5.3 6.3

Recovery

The recovery of recombinant human BMP-2 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
2 M Guanidine-HCI (n=1) 98 94-101
2 M Urea (n=1) 101 92-106
Cell Culture Media (n=4) 102 94-110

Linearity

The following samples were spiked with rhBMP-2 and then diluted with Calibrator Diluent. The samples were then serially diluted and tested to assess the linearity of the assay.
Human/Mouse/Rat BMP-2 ELISA Linearity

Data Examples

Human/Mouse/Rat BMP-2 ELISA Standard Curve

Product Datasheets

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: BMP-2

BMPs are secreted signaling molecules that comprise a subfamily of the TGF-beta superfamily and were originally identified as regulators of cartilage and bone formation. There are at least 20 structurally and functionally related BMPs, most of which play roles in embryogenesis and morphogenesis of various tissues and organs. Biologically active BMPs are usually homodimers containing a characteristic cysteine knot structure. Heterodimers, BMP-2/BMP-7 and BMP-4/BMP-7 have also been suggested to exist and function in vivo. They are more potent inducers of bone formation than their respective homodimers. In addition, heterodimers, but not homodimers, are ventral mesoderm inducers. Heterodimer activity may be mediated by a different or additional receptor subtype.

Decapentaplegic (Dpp) is one of at least five TGF-beta superfamily ligands identified in the Drosophila genome. Dpp, a functional ortholog of mammalian BMP-2 and BMP-4, is a morphogen and plays an essential role in Drosophila development. Dpp regulates embryonic dorsal-ventral polarity and is required for gut morphogenesis and outgrowth and patterning of imaginal disks.

Long Name:
Bone Morphogenetic Protein 2
Entrez Gene IDs:
650 (Human); 12156 (Mouse); 29373 (Rat)
Alternate Names:
BDA2; BMP2; BMP-2; BMP-2A; BMP2ABone morphogenetic protein 2A; bone morphogenetic protein 2; SSFSC
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for BMP-2 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

40 Citations: Showing 1 - 10
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  1. A comparison of BMP2 delivery by coacervate and gene therapy for promoting human muscle-derived stem cell-mediated articular cartilage repair
    Authors: X Gao, H Cheng, H Awada, Y Tang, S Amra, A Lu, X Sun, G Lv, C Huard, B Wang, X Bi, Y Wang, J Huard
    Stem Cell Res Ther, 2019;10(1):346.
    Species: Human
    Sample Types: Cell Culture Supernates
  2. Bone morphogenetic protein activity preservation with extracorporeal irradiation- and liquid nitrogen freezing-treated recycled autografts for biological reconstruction in malignant bone tumor
    Authors: CM Chen, CF Chen, JY Wang, R Madda, SW Tsai, PK Wu, WM Chen
    Cryobiology, 2019;0(0):.
    Species: Human
    Sample Types: Bone
  3. Regulated ex vivo regional gene therapy for bone repair using an inducible caspase-9 suicide gene system
    Authors: S Bougioukli, V Vakhshori, B Ortega, O Sugiyama, J Lieberman
    Gene Ther., 2019;0(0):.
    Species: Rat
    Sample Types: Cell Culture Supernates
  4. Synovium extra cellular matrices seeded with transduced mesenchymal stem cells stimulate chondrocyte maturation in vitro and cartilage healing in clinically-induced rat-knee lesions in vivo
    Authors: NA Reisbig, E Pinnell, L Scheuerman, H Hussein, AL Bertone
    PLoS ONE, 2019;14(3):e0212664.
    Species: Equine
    Sample Types: Cell Culture Supernates
  5. Gene activated adipose tissue fragments as advanced autologous biomaterials for bone regeneration: osteogenic differentiation within the tissue and implications for clinical translation
    Authors: B Ren, VM Betz, C Thirion, M Salomon, RM Klar, V Jansson, PE Müller, OB Betz
    Sci Rep, 2019;9(1):224.
    Species: Human
    Sample Types: Cell Culture Supernates
  6. Influences of donor and host age on human muscle-derived stem cell-mediated bone regeneration
    Authors: X Gao, A Lu, Y Tang, J Schneppend, AB Liebowitz, AC Scibetta, ER Morris, H Cheng, C Huard, S Amra, B Wang, MA Hall, WR Lowe, J Huard
    Stem Cell Res Ther, 2018;9(1):316.
    Species: Human
    Sample Types: Cell Culture Supernates
  7. Nanoparticle-modified chitosan-agarose-gelatin scaffold for sustained release of SDF-1 and BMP-2
    Authors: B Wang, Y Guo, X Chen, C Zeng, Q Hu, W Yin, W Li, H Xie, B Zhang, X Huang, F Yu
    Int J Nanomedicine, 2018;13(0):7395-7408.
    Species: Human
    Sample Types: Cell Culture Supernates
  8. Osteoinduction within BMP-2 transduced muscle tissue fragments with and without a fascia layer: implications for bone tissue engineering
    Authors: B Ren, VM Betz, C Thirion, M Salomon, V Jansson, PE Müller, OB Betz
    Gene Ther., 2018;0(0):.
    Species: Rat
    Sample Types: Cell Culture Supernates
  9. Differential effects of amnion and chorion membrane extracts on osteoblast-like cells due to the different growth factor composition of the extracts
    Authors: YY Go, SE Kim, GJ Cho, SW Chae, JJ Song
    PLoS ONE, 2017;12(8):e0182716.
    Species: Human
    Sample Types: Tissue Homogenates
  10. Inhibiting PHD2 in bone marrow mesenchymal stem cells via lentiviral vector-mediated RNA interference facilitates the repair of periodontal tissue defects in SD rats
    Authors: C Chen, H Li, J Jiang, Q Zhang, F Yan
    Oncotarget, 2017;8(42):72676-72699.
    Species: Rat
    Sample Types: Cell Culture Supernates
  11. BMP2-modified injectable hydrogel for osteogenic differentiation of human periodontal ligament stem cells
    Authors: SH Park, JS Kwon, BS Lee, JH Park, BK Lee, JH Yun, BY Lee, JH Kim, BH Min, TH Yoo, MS Kim
    Sci Rep, 2017;7(1):6603.
    Species: Human
    Sample Types: Protein
  12. Bone morphogenetic protein-2 loaded poly(D,L-lactide-co-glycolide) microspheres enhance osteogenic potential of gelatin/hydroxyapatite/?-tricalcium phosphate cryogel composite for alveolar ridge augmentation
    Authors: HC Chang, C Yang, F Feng, FH Lin, CH Wang, PC Chang
    J. Formos. Med. Assoc, 2017;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
  13. Inflammatory cell response to ultra-thin amorphous and crystalline hydroxyapatite surfaces
    Authors: Louise Rydén
    J Mater Sci Mater Med, 2017;28(1):9.
    Species: Human
    Sample Types: Cell Culture Supernates
  14. Facilitated receptor-recognition and enhanced bioactivity of bone morphogenetic protein-2 on magnesium-substituted hydroxyapatite surface
    Authors: B Huang, Y Yuan, T Li, S Ding, W Zhang, Y Gu, C Liu
    Sci Rep, 2016;6(0):24323.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  15. Targeted next-generation sequencing reveals multiple deleterious variants in OPLL-associated genes
    Authors: Xin Chen
    Sci Rep, 2016;6(0):26962.
    Species: Human
    Sample Types: Plasma
  16. Modified mRNA for BMP 2 in combination with biomaterials serves as a transcript activated matrix for effectively inducing osteogenic pathways in stem cells
    Stem Cells Dev, 2016;0(0):.
    Species: Rat
    Sample Types: Cell Culture Supernates
  17. Dental follicle stem cells in bone regeneration on titanium implants.
    Authors: Lucaciu O, Soritau O, Gheban D, Ciuca D, Virtic O, Vulpoi A, Dirzu N, Campian R, Baciut G, Popa C, Simon S, Berce P, Baciut M, Crisan B
    BMC Biotechnol, 2015;15(1):114.
    Species: Human
    Sample Types: Cell Culture Supernates
  18. Identifying an ovarian cancer cell hierarchy regulated by bone morphogenetic protein 2.
    Authors: Choi Y, Ingram P, Yang K, Coffman L, Iyengar M, Bai S, Thomas D, Yoon E, Buckanovich R
    Proc Natl Acad Sci U S A, 2015;112(50):E6882-8.
    Species: Human
    Sample Types: Cell Culture Supernates
  19. Increased plasma BMP-2 levels are associated with atherosclerosis burden and coronary calcification in type 2 diabetic patients.
    Authors: Zhang M, Sara J, Wang F, Liu L, Su L, Zhe J, Wu X, Liu J
    Cardiovasc Diabetol, 2015;14(0):64.
    Species: Human
    Sample Types: Plasma
  20. Disequilibrium of BMP2 levels in the breast stem cell niche launches epithelial transformation by overamplifying BMPR1B cell response.
    Authors: Chapellier M, Bachelard-Cascales E, Schmidt X, Clement F, Treilleux I, Delay E, Jammot A, Menetrier-Caux C, Pochon G, Besancon R, Voeltzel T, Caron de Fromentel C, Caux C, Blay J, Iggo R, Maguer-Satta V
    Stem Cell Reports, 2015;4(2):239-54.
    Species: Human
    Sample Types: Tissue Homogenates
  21. The role of transduced bone marrow cells overexpressing BMP-2 in healing critical-sized defects in a mouse femur.
    Authors: Pensak M, Hong S, Dukas A, Tinsley B, Drissi H, Tang A, Cote M, Sugiyama O, Lichtler A, Rowe D, Lieberman J
    Gene Ther, 2015;22(6):467-75.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  22. Asparagine-linked glycosylation of bone morphogenetic protein-2 is required for secretion and osteoblast differentiation.
    Authors: Hang, Qinglei, Zhou, Ying, Hou, Sicong, Zhang, Dongmei, Yang, Xiaojing, Chen, Jianping, Ben, Zhiyun, Cheng, Chun, Shen, Aiguo
    Glycobiology, 2014;24(3):292-304.
    Species: Hamster
    Sample Types: Cell Lysates
  23. Simple signaling molecules for inductive bone regenerative engineering.
    Authors: Cushnie, Emily K, Ulery, Bret D, Nelson, Stephen, Deng, Meng, Sethuraman, Swaminat, Doty, Stephen, Lo, Kevin W, Khan, Yusuf M, Laurencin, Cato T
    PLoS ONE, 2014;9(7):e101627.
    Species: Human
    Sample Types: Cell Culture Supernates
  24. Neuroprotection by rAAV-mediated gene transfer of bone morphogenic protein 7.
    Authors: Heinonen A, Rahman M, Dogbevia G, Jakobi H, Wolfl S, Sprengel R, Schwaninger M
    BMC Neurosci, 2014;15(0):38.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  25. Designing nanotopographical density of extracellular matrix for controlled morphology and function of human mesenchymal stem cells.
    Authors: Kim J, Kim H, Lim K, Kim Y, Seonwoo H, Park S, Lim H, Kim D, Suh K, Choung P, Choung Y, Chung J
    Sci Rep, 2013;3(0):3552.
    Species: Human
    Sample Types: Cell Culture Supernates
  26. Bone morphogenetic protein-2/-4 upregulation promoted by endothelial cells in coculture enhances mouse embryoid body differentiation.
    Authors: Talavera-Adame, Dodanim, Gupta, Ankur, Kurtovic, Silvia, Chaiboonma, Kira L, Arumugaswami, Vaithili, Dafoe, Donald C
    Stem Cells Dev, 2013;22(24):3252-60.
    Species: Human
    Sample Types: Cell Culture Supernates
  27. The serum bone morphogenetic protein-2 level in non-small-cell lung cancer patients.
    Authors: Choi YJ, Kim ST, Park KH, Oh SC, Seo JH, Shin SW, Kim JS, Kim YH
    Med. Oncol., 2012;29(0):582-8.
    Species: Human
    Sample Types: Serum
  28. Bone morphogenetic protein-2 may represent the molecular link between oxidative stress and vascular stiffness in chronic kidney disease.
    Authors: Dalfino G, Simone S, Porreca S, Cosola C, Balestra C, Manno C, Schena FP, Grandaliano G, Pertosa G
    Atherosclerosis, 2010;211(2):418-23.
    Species: Human
    Sample Types: Serum
  29. Association of bone morphogenetic proteins with spinal fusion in ankylosing spondylitis.
    Authors: Chen HA, Chen CH, Lin YJ, Chen PC, Chen WS, Lu CL, Chou CT
    J. Rheumatol., 2010;37(10):2126-32.
    Species: Human
    Sample Types: Serum
  30. Periodontal regeneration using ex vivo autologous stem cells engineered to express the BMP-2 gene: an alternative to alveolaplasty.
    Authors: Chen YL, Chen PK, Jeng LB, Huang CS, Yang LC, Chung HY, Chang SC
    Gene Ther., 2008;15(22):1469-77.
    Species: Rabbit
    Sample Types: Cell Culture Supernates
  31. Bone marrow cells are a rich source of growth factors and cytokines: implications for cell therapy trials after myocardial infarction.
    Authors: Korf-Klingebiel M, Kempf T, Sauer T, Brinkmann E, Fischer P, Meyer GP, Ganser A, Drexler H, Wollert KC
    Eur. Heart J., 2008;29(23):2851-8.
    Species: Human
    Sample Types: Cell Culture Supernates
  32. Isolation and characterization of CD146+ multipotent mesenchymal stromal cells.
    Authors: Sorrentino A, Ferracin M, Castelli G, Biffoni M, Tomaselli G, Baiocchi M, Fatica A, Negrini M, Peschle C, Valtieri M
    Exp. Hematol., 2008;36(8):1035-46.
    Species: Human
    Sample Types: Cell Culture Supernates
  33. Mesenchymal stem cells support migration, extracellular matrix invasion, proliferation, and survival of endothelial cells in vitro.
    Authors: Potapova IA, Gaudette GR, Brink PR, Robinson RB, Rosen MR, Cohen IS, Doronin SV
    Stem Cells, 2007;25(7):1761-8.
    Species: Human
    Sample Types: Cell Culture Supernates
  34. Activin-like kinase receptor 1 (ALK1) in atherosclerotic lesions and vascular mesenchymal cells.
    Authors: Yao Y, Zebboudj AF, Torres A, Shao E, Bostrom K
    Cardiovasc. Res., 2007;74(2):279-89.
    Species: Human
    Sample Types: Cell Culture Supernates
  35. Quantitative assessment of growth factors in reaming aspirate, iliac crest, and platelet preparation.
    Authors: Herrmann S, Green J, Weber T, Scharfenberger A
    Bone, 2006;39(5):1156-63.
    Species: Human
    Sample Types: Tissue Homogenates
  36. Targeted delivery system for juxtacrine signaling growth factor based on rhBMP-2-mediated carrier-protein conjugation.
    Authors: Liu HW, Chen CH, Tsai CL, Hsiue GH
    Bone, 2006;39(4):825-36.
    Species: Human
    Sample Types: Recombinant Protein
  37. Direct adenoviral transfer of bone morphogenetic protein-2 cDNA enhances fracture healing in osteoporotic sheep.
    Authors: Egermann M, Baltzer AW, Adamaszek S, Evans C, Robbins P, Schneider E, Lill CA
    Hum. Gene Ther., 2006;17(5):507-17.
    Species: Ovine
    Sample Types: Cell Culture Supernates
  38. Combinatorial gene therapy for bone regeneration: cooperative interactions between adenovirus vectors expressing bone morphogenetic proteins 2, 4, and 7.
    Authors: Zhao M, Zhao Z, Koh JT, Jin T, Franceschi RT
    J. Cell. Biochem., 2005;95(1):1-16.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  39. Measurement of bone morphogenetic proteins and other growth factors in demineralized bone matrix.
    Authors: Blum B, Moseley J, Miller L, Richelsoph K, Haggard W
    Orthopedics, 2004;27(1):161-5.
    Species: Rat
    Sample Types: Tissue Homogenates
  40. Shock wave application enhances pertussis toxin protein-sensitive bone formation of segmental femoral defect in rats.
    Authors: Chen YJ, Kuo YR, Yang KD, Wang CJ, Huang HC, Wang FS
    J. Bone Miner. Res., 2003;18(12):2169-79.
    Species: Rat
    Sample Types: Serum

FAQs

  1. Does the BMP-2 Quantikine® ELISA kit (Catalog # DBP200) recognize all forms of BMP-2? Does this kit detect the BMP-2/BMP-7 heterodimer?

     

    • The BMP-2 Quantikine® ELISA kit (Catalog # DBP200) is specific to mammalian expressed BMP-2. Recombinant E.coli expressed BMP-2 (Catalog # 355-BEC) is not detected in this assay. Cross-reactivity information is provided under the specificity section of the kit booklet. This kit does not detect the BMP-2/BMP-7 heterodimer.

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Reviews for BMP-2 Quantikine ELISA Kit

Average Rating: 4.7 (Based on 7 Reviews)

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BMP-2 Quantikine ELISA Kit
By Anonymous on 10/10/2019
Application: Sample Tested: Bone Extracts

BMP-2 Quantikine ELISA Kit
By Anonymous on 08/24/2017
Application: Sample Tested: scaffolds loaded with BMP2

BMP-2 Quantikine ELISA Kit
By Anonymous on 05/12/2016
Application: Sample Tested: Bone Extracts

BMP-2 Quantikine ELISA Kit
By Anonymous on 05/09/2016
Application: Sample Tested: Cartilage tissue

BMP-2 Quantikine ELISA Kit
By Anonymous on 05/05/2016
Application: Sample Tested: Bone Extracts

BMP-2 Quantikine ELISA Kit
By Anonymous on 05/05/2016
Application: Sample Tested: Human tissue

BMP-2 Quantikine ELISA Kit
By Anonymous on 05/04/2016
Application: Sample Tested: Bone Extracts