BMX Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-84778

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all BMX Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KFLCCQQSCKAAPGCTLWEAYANLHTAVNEEKHRVPTFPDRVLKIPRAVPVLKMDAPSSSTTLAQYDNESKKNYGSQPPSSSTSLAQYDSNSKKIYGSQPNFNMQYIPREDFPDWWQVR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BMX Antibody - BSA Free

Immunohistochemistry-Paraffin: BMX Antibody [NBP1-84778]

Immunohistochemistry-Paraffin: BMX Antibody [NBP1-84778]

Immunohistochemistry-Paraffin: BMX Antibody [NBP1-84778] - Staining in human epididymis and fallopian tube tissues using anti-BMX antibody. Corresponding BMX RNA-seq data are presented for the same tissues.
Western Blot: BMX Antibody [NBP1-84778]

Western Blot: BMX Antibody [NBP1-84778]

Western Blot: BMX Antibody [NBP1-84778] - Analysis in control (vector only transfected HEK293T lysate) and BMX over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: BMX Antibody [NBP1-84778]

Immunohistochemistry-Paraffin: BMX Antibody [NBP1-84778]

Immunohistochemistry-Paraffin: BMX Antibody [NBP1-84778] - Staining of human fallopian tube shows low expression as expected.
BMX Antibody - BSA Free Immunohistochemistry-Paraffin: BMX Antibody - BSA Free [NBP1-84778]

Immunohistochemistry-Paraffin: BMX Antibody - BSA Free [NBP1-84778]

Staining of human epididymis shows high expression.

Applications for BMX Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BMX

The Tec family of non-receptor tyrosine kinases is composed of six proteins designated Tec, Emt (also known as Itk or Tsk), Btk (previously known as Atk, BPK or Emb), Bmx, Txk (also known as Rlk) and Dsrc28C. All members of the family contain SH3 and SH2 domains and, with the exception of Txk and Dsrc28C, also contain a pleckstrin homology (PH) and a Tec homology (TH) domain in their amino termini. Four alternatively spliced forms of Tec are found to be expressed broadly in cells of hematopoietic lineage and hepatocytes. The 72 kDa Emt gene product associates with CD28 and becomes activated subsequent to CD28 ligation. Btk is necessary for proper B cell development, and mutations in the gene encoding Btk have been associated with families suffering from X-linked agammaglobulinemia, also referred to as Bruton's disease. The 80 kDa Bmx protein shares a high degree of homology with Btk and seems to be expressed at highest levels in the heart. Txk expression is T cell-specific, while expression of the Drosophila Tec homolog, Dsrc28C, is developmentally regulated.

Long Name

BMX Non-receptor Tyrosine Kinase

Alternate Names

ETK, NTK38, PSCTK3

Gene Symbol

BMX

Additional BMX Products

Product Documents for BMX Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for BMX Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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