BNIP3 Antibody - BSA Free
Novus Biologicals | Catalog # NB100-56150
![Western Blot: BNIP3 Antibody [NB100-56150]](https://resources.rndsystems.com/images/products/BNIP3-Antibody-Western-Blot-NB100-56150-img0001.jpg "Western Blot: BNIP3 Antibody [NB100-56150]")
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Key Product Details
Species Reactivity
Validated:
Human, Rat, Porcine, Chicken, Mouse (Negative), Xenopus
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
A synthetic peptide corresponding to amino acids 122-139 (SRPENIPPKEFLFKHPKR) of human NIP3 was used aas immunogen; GenBank no. gi|7669481|ref|NP_004043.2|.
Reactivity Notes
94% sequence identity with Canine protein. Does not work in mouse.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for BNIP3 Antibody - BSA Free
Western Blot: BNIP3 Antibody [NB100-56150]
Western Blot: BNIP3 Antibody [NB100-56150] - BNIP3 expression in porcine whole skeletal muscle lysate. Image from verified customer review.![Immunohistochemistry-Paraffin: BNIP3 Antibody [NB100-56150]](https://resources.rndsystems.com/images/products/BNIP3-Antibody-Immunohistochemistry-Paraffin-NB100-56150-img0002.jpg "Immunohistochemistry-Paraffin: BNIP3 Antibody [NB100-56150]")
Immunohistochemistry-Paraffin: BNIP3 Antibody [NB100-56150]
Immunohistochemistry-Paraffin: BNIP3 Antibody - Unpurified [NB100-56150] - BNIP3 Antibody [NB100-56150] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human liver using BNIP3 antibody at 1:1000 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Mitochondrial staining was observed. Staining was performed by Histowiz.Applications for BNIP3 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:1000-1:5000
Western Blot
1:1000-1:2000
Reviewed Applications
Read 1 review rated 4 using NB100-56150 in the following applications:
Formulation, Preparation, and Storage
Purification
Unpurified
Formulation
Whole antisera
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: BNIP3
Long Name
BCL2/Adenovirus E1B 19 Kda Protein-Interacting Protein 3-Like
Alternate Names
Nip3
Gene Symbol
BNIP3
Additional BNIP3 Products
Product Documents for BNIP3 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for BNIP3 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for BNIP3 Antibody - BSA Free
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Customer Reviews for BNIP3 Antibody - BSA Free (1)
4 out of 5
1 Customer Rating
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Application: Western BlotSample Tested: Whole Skeletal Muscle LysateSpecies: OtherVerified Customer | Posted 04/03/2015Whole Skeletal Muscle Lysate
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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