Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # BOB1/2424

Format

Azide and BSA Free
View all BOB1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment (around aa 148-255) of human BOB1 protein (exact sequence is proprietary) (Uniprot: Q16633)

Localization

Nuclear

Marker

B-Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Theoretical MW

35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-75752).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for BOB1 Antibody (BOB1/2424) - Azide and BSA Free

Western Blot: BOB1 Antibody (BOB1/2424)Azide and BSA Free [NBP2-75753]

Western Blot: BOB1 Antibody (BOB1/2424)Azide and BSA Free [NBP2-75753]

Western Blot: BOB1 Antibody (BOB1/2424) - Azide and BSA Free [NBP2-75753] - Western Blot Analysis of Raji cell lysate using BOB1 Monospecific Mouse Monoclonal Antibody (BOB1/2424).
Immunocytochemistry/ Immunofluorescence: BOB1 Antibody (BOB1/2424) - Azide and BSA Free [NBP2-75753]

Immunocytochemistry/ Immunofluorescence: BOB1 Antibody (BOB1/2424) - Azide and BSA Free [NBP2-75753]

Immunocytochemistry/Immunofluorescence: BOB1 Antibody (BOB1/2424) - Azide and BSA Free [NBP2-75753] - Immunofluorescent staining of paraformaldehyde-fixed Raji cells using BOB1 Monospecific Mouse Monoclonal Antibody (BOB1/2424) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are labeled with RedDot (red).
Immunohistochemistry-Paraffin: BOB1 Antibody (BOB1/2424) - Azide and BSA Free [NBP2-75753]

Immunohistochemistry-Paraffin: BOB1 Antibody (BOB1/2424) - Azide and BSA Free [NBP2-75753]

Immunohistochemistry-Paraffin: BOB1 Antibody (BOB1/2424) - Azide and BSA Free [NBP2-75753] - Formalin-fixed, paraffin-embedded human Spleen stained with BOB1 Monospecific Mouse Monoclonal Antibody (BOB1/2424).
Flow Cytometry: BOB1 Antibody (BOB1/2424) - Azide and BSA Free [NBP2-75753]

Flow Cytometry: BOB1 Antibody (BOB1/2424) - Azide and BSA Free [NBP2-75753]

Flow Cytometry: BOB1 Antibody (BOB1/2424) - Azide and BSA Free [NBP2-75753] - Flow Cytometric Analysis of paraformaldehyde-fixed Raji cells. BOB1 Monospecific Mouse Monoclonal Antibody (BOB1/2424).; followed by goat anti-Mouse IgG-CF488 (blue) and Isotype Control (Red).
Protein Array: BOB1 Antibody (BOB1/2424) - Azide and BSA Free [NBP2-75753]

Protein Array: BOB1 Antibody (BOB1/2424) - Azide and BSA Free [NBP2-75753]

Protein Array: BOB1 Antibody (BOB1/2424) - Azide and BSA Free [NBP2-75753] - Analysis of Protein Array containing >19,000 full-length human proteins using BOB1 Monospecific Mouse Monoclonal Antibody (BOB1/2424)

Applications for BOB1 Antibody (BOB1/2424) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1 - 2 ug/mL

Immunocytochemistry/ Immunofluorescence

1 - 2 ug/mL

Immunohistochemistry

1 - 2 ug/mL

Immunohistochemistry-Paraffin

1 - 2 ug/mL

Western Blot

1 - 2 ug/mL
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: BOB1

BOB1 is a novel 35kDa protein designated Bob1 (B cell Oct binding protein 1) is a lymphocyte specific transcription co activator protein. B cell specific transcription is mediated by octamer motifs found in immunoglobulin heavy and light chain gene promoters and in some immunoglobulin enhancers. These sites are bound by the B cell specific Oct1 and Oct2 transcription factors, and the B cell restricted co activator BOB.1/OBF.1. This protein interacts only with the Oct1/2 proteins through sub domains in the POU domain of the Oct1/2 proteins, enhancing their transcriptional efficacy. Although having no intrinsic capacity for DNA binding, Bob1 associates tightly with the octomer motif in the presence of Oct1 and Oct2. Bob1 is expressed at highest levels in spleen and peripheral blood leukocytes.

Alternate Names

B-cell-specific coactivator OBF-1, BOB-1, OBF-1, OBF1BOB1, OCAB, OCA-B, OCT-binding factor 1, POU class 2 associating factor 1, POU domain class 2, associating factor 1, POU domain class 2-associating factor 1, POU domain, class 2, associating factor 1

Gene Symbol

POU2AF1

UniProt

Q16633 (Human)

Additional BOB1 Products

Product Documents for BOB1 Antibody (BOB1/2424) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for BOB1 Antibody (BOB1/2424) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for BOB1 Antibody (BOB1/2424) - Azide and BSA Free

There are currently no reviews for this product. Be the first to review BOB1 Antibody (BOB1/2424) - Azide and BSA Free and earn rewards!

Have you used BOB1 Antibody (BOB1/2424) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies