Boll Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-33538

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (94%), Rat (95%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Boll Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: VTFETQEDAQKILQEAEKLNYKDKKLNIGPAIRKQQVGIPRSSIMPAAGTMYLTTSTGYPYTYHNGVAYFHTPEVTSVPPPWPSRSVCSSPVMVAQPIYQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Boll Antibody - BSA Free

Western Blot: Boll Antibody [NBP2-33538]

Western Blot: Boll Antibody [NBP2-33538]

Western Blot: Boll Antibody [NBP2-33538] - Analysis in control (vector only transfected HEK293T lysate) and BOLL over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: Boll Antibody [NBP2-33538]

Immunohistochemistry-Paraffin: Boll Antibody [NBP2-33538]

Immunohistochemistry-Paraffin: Boll Antibody [NBP2-33538] - Staining of human lymph node.
Immunohistochemistry-Paraffin: Boll Antibody [NBP2-33538]

Immunohistochemistry-Paraffin: Boll Antibody [NBP2-33538]

Immunohistochemistry-Paraffin: Boll Antibody [NBP2-33538] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: Boll Antibody [NBP2-33538]

Immunohistochemistry-Paraffin: Boll Antibody [NBP2-33538]

Immunohistochemistry-Paraffin: Boll Antibody [NBP2-33538] - Staining of human kidney shows low expression as expected.
Immunohistochemistry-Paraffin: Boll Antibody [NBP2-33538]

Immunohistochemistry-Paraffin: Boll Antibody [NBP2-33538]

Immunohistochemistry-Paraffin: Boll Antibody [NBP2-33538] - Staining of human testis shows high expression.
Immunohistochemistry-Paraffin: Boll Antibody [NBP2-33538]

Immunohistochemistry-Paraffin: Boll Antibody [NBP2-33538]

Immunohistochemistry-Paraffin: Boll Antibody [NBP2-33538] - Staining in human testis and kidney tissues using anti-BOLL antibody. Corresponding BOLL RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Boll Antibody [NBP2-33538]

Immunohistochemistry-Paraffin: Boll Antibody [NBP2-33538]

Immunohistochemistry-Paraffin: Boll Antibody [NBP2-33538] - Staining of human colon, liver, lymph node and testis using Anti-BOLL antibody NBP2-33538 (A) shows similar protein distribution across tissues to independent antibody NBP1-88377 (B).
Immunohistochemistry-Paraffin: Boll Antibody [NBP2-33538]

Immunohistochemistry-Paraffin: Boll Antibody [NBP2-33538]

Immunohistochemistry-Paraffin: Boll Antibody [NBP2-33538] - Staining of human liver.
Immunohistochemistry-Paraffin: Boll Antibody [NBP2-33538]

Immunohistochemistry-Paraffin: Boll Antibody [NBP2-33538]

Immunohistochemistry-Paraffin: Boll Antibody [NBP2-33538] - Staining of human colon.

Applications for Boll Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:2500 - 1:5000

Immunohistochemistry-Paraffin

1:2500 - 1:5000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Boll

Boll belongs to the DAZ gene family required for germ cell development. It encodes an RNA-binding protein which is more similar to Drosophila Boule than to human proteins encoded by genes DAZ (deleted in azoospermia) or DAZL (deleted in azoospermia-like). Loss of this gene function results in the absence of sperm in semen (azoospermia). Histological studies demonstrated that the primary defect is at the meiotic G2/M transition. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Alternate Names

bol, boule-like (Drosophila), BOULEbol (Drosophila boule homolog)-like, protein boule-like

Gene Symbol

BOLL

UniProt

Q8N9W6 (Human)

Additional Boll Products

Product Documents for Boll Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Boll Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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