BOP1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-12594

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all BOP1 Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human BOP1 fragment

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Protein A & Antigen Affinity purified

Scientific Data Images for BOP1 Antibody - BSA Free

Western Blot: BOP1 Antibody [NBP3-12594]

Western Blot: BOP1 Antibody [NBP3-12594]

Western Blot: BOP1 Antibody [NBP3-12594] - Lane A: HeLa Whole Cell LysateLane B: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:94 kDaObserved band size:100 kDa(We are unsure as to the identity of these extra bands.)
BOP1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: BOP1 Antibody - BSA Free [NBP3-12594] -

Immunocytochemistry/ Immunofluorescence: BOP1 Antibody - BSA Free [NBP3-12594] - Immunofluorescence staining of BOP1 in PC3 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-12594 (dilution ratio 1:200) at 4 overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Nucleus.
BOP1 Antibody - BSA Free

Immunoprecipitation: BOP1 Antibody - BSA Free [NBP3-12594] -

Immunoprecipitation: BOP1 Antibody - BSA Free [NBP3-12594] - BOP1 was immunoprecipitated using: Lane A:0.5 mg HeLa Whole Cell Lysate 4 uL NBP3-12594 and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: NBP3-12594, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) [HRP] at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 94 kDa Observed band size :100 kDa

Applications for BOP1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunoprecipitation

1-5ul/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Format

BSA Free

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BOP1

BOP1 (block of proliferation 1) is a component of the PeBoW (Pes1, Bop, WDR12) complex that plays a role in the processing of 28S and 5.8S ribosomal RNAs and the formation of the 60S ribosome. BOP1 contains seven WD repeats and was originally identified as a cDNA which inhibited cell proliferation. The deregulation of the BOP1 gene is proposed to contribute to colorectal tumorigenesis.

Alternate Names

block of proliferation 1, KIAA0124Block of proliferation 1 protein, ribosome biogenesis protein BOP1

Gene Symbol

BOP1

Additional BOP1 Products

Product Documents for BOP1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for BOP1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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