Borealin Antibody - BSA Free

Western Blot: Borealin Antibody - BSA Free [NBP1-89951]

Analysis in control (vector only transfected HEK293T lysate) and CDCA8 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).

Western Blot: Borealin Antibody [NBP1-89951] -

Western Blot: Borealin Antibody [NBP1-89951] - Aurora B preferentially binds to H3R2me2a & recruits CPC components.a The amino acid sequences of modified histone H3 peptides. b In vitro Haspin kinase assay w/ 21-aa peptide. c In vitro Aurora B kinase assay w/ 21-aa peptide for phosphorylation. d In vitro PRMT6 methyltransferase assay. e–h The cells treated w/ siPRMT6, PRMT6 inhibitor MS023, or the Haspin kinase inhibitor CHR-6494. The intensities of H3R2me2a (f) & H3T3ph (h) in chromosome arm & centromere analyzed by IF microscopy of nocodazole-arrested HeLa chromosome spreads & plotted (n = 100 chromosomes from three independent experiments). i After transfection of indicated plasmids, HeLa cells treated w/ a Haspin kinase inhibitor, CHR-6494, for 5 h & the intensity of CPC components analyzed for 100 centromeres from three independent experiments. j Recombinant CPC proteins incubated w/ biotinylated histone peptides & pulled down w/ streptavidin-agarose beads, & the binding visualized by immunoblotting. k Lysates of TN-arrested Aurora B-depleted cells supplemented w/ recombinant Survivin & Borealin proteins w/ or w/out recombinant Aurora B protein. The lysates incubated w/ antibodies against INCENP & the H3R2me2a peptide, & pulldown conducted w/ agarose A beads. The binding visualized by immunoblotting. The asterisk denotes the light chain of antibodies. l Lysates of TN-arrested mitotic HeLa cells incubated w/ biotinylated histone peptides & pulled down w/ streptavidin-agarose beads, & the binding visualized by immunoblotting. Relative band intensities of band measured w/ image processing software (Image Studio ver5.0). Error bars, SEMs. Scale bars, 5 μm. Source data are provided as a Source Data file. (Student’s t-test *p < 0.01). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32001712), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Borealin Antibody [NBP1-89951]

Borealin-Antibody-Western-Blot-NBP1-89951-img0018.jpg

Western Blot: Borealin Antibody [NBP1-89951]

Borealin-Antibody-Western-Blot-NBP1-89951-img0017.jpg

Western Blot: Borealin Antibody [NBP1-89951] -

Western Blot: Borealin Antibody [NBP1-89951] - Aurora B preferentially binds to H3R2me2a & recruits CPC components.a The amino acid sequences of modified histone H3 peptides. b In vitro Haspin kinase assay w/ 21-aa peptide. c In vitro Aurora B kinase assay w/ 21-aa peptide for phosphorylation. d In vitro PRMT6 methyltransferase assay. e–h The cells treated w/ siPRMT6, PRMT6 inhibitor MS023, or the Haspin kinase inhibitor CHR-6494. The intensities of H3R2me2a (f) & H3T3ph (h) in chromosome arm & centromere analyzed by IF microscopy of nocodazole-arrested HeLa chromosome spreads & plotted (n = 100 chromosomes from three independent experiments). i After transfection of indicated plasmids, HeLa cells treated w/ a Haspin kinase inhibitor, CHR-6494, for 5 h & the intensity of CPC components analyzed for 100 centromeres from three independent experiments. j Recombinant CPC proteins incubated w/ biotinylated histone peptides & pulled down w/ streptavidin-agarose beads, & the binding visualized by immunoblotting. k Lysates of TN-arrested Aurora B-depleted cells supplemented w/ recombinant Survivin & Borealin proteins w/ or w/out recombinant Aurora B protein. The lysates incubated w/ antibodies against INCENP & the H3R2me2a peptide, & pulldown conducted w/ agarose A beads. The binding visualized by immunoblotting. The asterisk denotes the light chain of antibodies. l Lysates of TN-arrested mitotic HeLa cells incubated w/ biotinylated histone peptides & pulled down w/ streptavidin-agarose beads, & the binding visualized by immunoblotting. Relative band intensities of band measured w/ image processing software (Image Studio ver5.0). Error bars, SEMs. Scale bars, 5 μm. Source data are provided as a Source Data file. (Student’s t-test *p < 0.01). Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32001712), licensed under a CC-BY license. Not internally tested by Novus Biologicals.