Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Cited:

Western Blot, Chemotaxis, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all BP1 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide made specifically to the human BP1 protein sequence (between residues 1-60) [UniProt# Q92988].

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: mouse (82%).

Localization

Nuclear.

Specificity

This is specific for human BP1 (DLX4 variant 1). Berg PE, Kirolikar S. DLX4 (distal-less homeobox 4). Atlas Genet Cytogenet Oncol Haematol. January 2011. URL : http://AtlasGeneticsOncology.org/Genes/DLX4ID49827ch17q21.html

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for BP1 Antibody - BSA Free

Western Blot: BP1 Antibody [NB100-481]

Western Blot: BP1 Antibody [NB100-481]

Western Blot: BP1 Antibody [NB100-481] - Detection of MDA-MB-231 cell lysates.
Immunohistochemistry-Frozen: BP1 Antibody [NB100-481]

Immunohistochemistry-Frozen: BP1 Antibody [NB100-481]

Immunohistochemistry-Frozen: BP1 Antibody [NB100-481] - Top Panel: BP1 antibody detected in breast cancer cells. Bottom Panel: Staining of normal breast tissue.
Western Blot: BP1 Antibody [NB100-481]

Western Blot: BP1 Antibody [NB100-481]

BP1-Antibody-Western-Blot-NB100-481-img0007.jpg
Immunoprecipitation: BP1 Antibody [NB100-481]

Immunoprecipitation: BP1 Antibody [NB100-481]

Immunoprecipitation: BP1 Antibody [NB100-481] - Antibody diluted 1:5,000 2. BP1-specific band after immunoprecipitation of 850 ug of cell extract using anti-BP1 (NB100-481) antibody diluted 1:1,000 3. Negative control for IP in lane 2: cell extract incubated with 50ul of Protein A/G beads.
BP1 Antibody

Western Blot: BP1 Antibody [NB100-481] -

Effect of BP1 on TNF alpha -induced cell death. (a) Western blot analysis of Beta Protein 1 (BP1) protein expression in MCF7/EV cell lines and MCF7/BP1 cell lines.

Applications for BP1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50-1:100

Immunohistochemistry-Paraffin

1:50-1:100

Immunoprecipitation

1:10-1:500

Western Blot

1:1000
Application Notes
This BP1 antibody is useful for Immunohistochemistry on paraffin-embedded sections, Immunoprecipitation and Western blot, where a band is seen at ~36 kDa. IP has been done with 35S-labeled BP1 protein only. It is not known if this antibody will immunoprecipitate a cell extract. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.Use in ChIP reported in scientific literature (PMID:35883028).

Reviewed Applications

Read 1 review rated 4 using NB100-481 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: BP1

BP1 (beta protein 1; also called distal-less homeobox gene 4 or DLX4) is a member of DLX family of homeobox genes and it controls many aspects of embryonic development including bone morphogenesis, skeletal patterning and cell differentiation. Originally discovered to be a repressor of beta-globin gene in erythroid cells, BP1 is normally expressed during early hematopoiesis with complete absence from most normal adult tissues and is widely re-expressed in leukemias, lung, breast, ovarian as well as prostate cancers. BP1 is an oncogene with a major role in breast cancer, and that its expression correlates with breast cancer progression and invasion. BP1 has been found to be detectable in 80% of infiltrating ductal carcinomas, but undetectable in matched normal controls, being the first DLX gene to be strongly implicated in breast cancer. Furthermore, expression of DLX4 correlates with estrogen receptor status and also cancer progression and invasion in breast cancer.

Alternate Names

Beta protein 1, BP1distal-less homeo box 7, distal-less homeobox 4, DLX7distal-less homeo box 9, DLX8DLX9, homeobox protein DLX-4, Homeobox protein DLX-7, Homeobox protein DLX-8

Entrez Gene IDs

1748 (Human)

Gene Symbol

DLX4

UniProt

Q92988 (Human)

Additional BP1 Products

Product Documents for BP1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for BP1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for BP1 Antibody - BSA Free

Customer Reviews for BP1 Antibody - BSA Free (1)

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  • Name: Patricia Berg
    Application: Western Blot
    Sample Tested: whole cell lysate, Sample Amount: 10-20ug/well
    Species: Human
    Verified Customer | Posted 08/10/2010

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