BRCC2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-92672

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all BRCC2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-108 of human BRCC2 (NP_001001786.2). MVTLLPIEGQEIHFFEILESECVLYTGWIERASGSSIYPEAKARLPLEALLGSNKEPMLPKETVLSLKRYNLGSSAMKRNVPGHVLQRPSYLTRIQVTLLCNSSAEAL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

12 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for BRCC2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: BRCC2 Antibody - BSA Free [NBP2-92672]

Immunocytochemistry/ Immunofluorescence: BRCC2 Antibody - BSA Free [NBP2-92672]

Immunocytochemistry/Immunofluorescence: BRCC2 Antibody [NBP2-92672] - Analysis of MCF-7 cells using BRCC2. Blue: DAPI for nuclear staining.
BRCC2 Antibody - BSA Free

Western Blot: BRCC2 Antibody - BSA Free [NBP2-92672] -

Western Blot: BRCC2 Antibody - BSA Free [NBP2-92672] - Western blot analysis of lysates from wild type (WT) and 293T cells transfected with BRCC2, using BRCC2 Rabbit pAb at 1:400 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.

Applications for BRCC2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:100

Western Blot

1:100 - 1:500

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: BRCC2

The BRCC2 gene encodes a BH3-like motif containing protein involved in cell death. The encoded protein may induce apoptosisin a caspase-dependent manner. The protein is localized in both the cytoplasm and the mitochondrion. (SH)

Alternate Names

BH3-like motif containing, cell death inducer, BRCC2BH3-like motif-containing cell death inducer, BRCCalpha, breast cancer cell 2, Breast cancer cell protein 2, MGC163233, MGC163235

Gene Symbol

BLID

Additional BRCC2 Products

Product Documents for BRCC2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for BRCC2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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