BRD4 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-86640

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Rat (94%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence, Simple Western, Chromatin Immunoprecipitation (ChIP)

Cited:

Chemotaxis

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all BRD4 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PQPAKPQQVIQHHHSPRHHKSDPYSTGHLREAPSPLMIHSPQMSQFQSLTHQSPPQQNVQPKKQELRAASVVQPQPLVVVKEEKIHSPIIRSEPFSPSLRPEPPKHPESIKAPVHLPQRPEMKPVDVGRPVIRPPEQNAPPP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BRD4 Antibody - BSA Free

BRD4 Antibody - BSA Free Immunohistochemistry-Paraffin: BRD4 Antibody - BSA Free [NBP1-86640]

Immunohistochemistry-Paraffin: BRD4 Antibody - BSA Free [NBP1-86640]

Staining of human cell line U-251 MG shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: BRD4 Antibody [NBP1-86640]

Immunohistochemistry-Paraffin: BRD4 Antibody [NBP1-86640]

Immunohistochemistry-Paraffin: BRD4 Antibody [NBP1-86640] - Staining of human tonsil shows moderate to strong nuclear positivity in non-germinal center cells.
Immunohistochemistry-Paraffin: BRD4 Antibody [NBP1-86640]

Immunohistochemistry-Paraffin: BRD4 Antibody [NBP1-86640]

Immunohistochemistry-Paraffin: BRD4 Antibody [NBP1-86640] - Staining of human cerebral cortex shows moderate to strong nuclear positivity in neurons.
Immunohistochemistry-Paraffin: BRD4 Antibody [NBP1-86640]

Immunohistochemistry-Paraffin: BRD4 Antibody [NBP1-86640]

Immunohistochemistry-Paraffin: BRD4 Antibody [NBP1-86640] - Staining of human placenta shows moderate to strong nuclear positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: BRD4 Antibody [NBP1-86640]

Immunohistochemistry-Paraffin: BRD4 Antibody [NBP1-86640]

Immunohistochemistry-Paraffin: BRD4 Antibody [NBP1-86640] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.
Simple Western: BRD4 Antibody [NBP1-86640]

Simple Western: BRD4 Antibody [NBP1-86640]

Simple Western: BRD4 Antibody [NBP1-86640] - Simple Western lane view shows a specific band for BRD4 in 0.2 mg/ml of U-251MG sp lysate. This experiment was performed under reducing conditions using the 66-440 kDa separation system.
Simple Western: BRD4 Antibody [NBP1-86640]

Simple Western: BRD4 Antibody [NBP1-86640]

Simple Western: BRD4 Antibody [NBP1-86640] - Electropherogram image(s) of corresponding Simple Western lane view. BRD4 antibody was used at 1:60 dilution on U-251MG sp lysates(s).

Applications for BRD4 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

Reported in scientific literature (PMID:25616107).

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Simple Western

1:60
Application Notes

ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100. IHC-Paraffin, HIER pH6 retrieval is recommended. Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in U-251MG sp, separated by Size, antibody dilution of 1:60, apparent MW was 274 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BRD4

BRD4 is encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described. [provided by RefSeq]

Long Name

Bromodomain Containing 4

Alternate Names

HUNK1, HUNKI, MCAP

Gene Symbol

BRD4

Additional BRD4 Products

Product Documents for BRD4 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for BRD4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Epigenetics

Citations for BRD4 Antibody - BSA Free

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Protocols

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