BRD9 Antibody (BLR153J)
Novus Biologicals | Catalog # NBP3-14730
Recombinant Monoclonal Antibody
![Western Blot: BRD9 Antibody (BLR153J) [NBP3-14730]](https://resources.rndsystems.com/images/products/BRD9-Antibody-BLR153J-Western-Blot-NBP3-14730-img0008.jpg "Western Blot: BRD9 Antibody (BLR153J) [NBP3-14730]")
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Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Simple Western, Immunoprecipitation
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit Clone # BLR153J
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Product Specifications
Immunogen
between 547 and 597 (C-term)
Clonality
Monoclonal
Host
Rabbit
Scientific Data Images for BRD9 Antibody (BLR153J)
Western Blot: BRD9 Antibody (BLR153J) [NBP3-14730]
Western Blot: BRD9 Antibody (BLR153J) [NBP3-14730] - Whole cell lysate (30 ug) from CT26, CH27, and TCMK-1 cellsprepared using NETN lysis buffer. Antibody: Rabbit antiBRD9 recombinant monoclonal antibody [BLR153J] used at 1:1000. Secondary: HRPconjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 3 minutes.Lower Panel: Rabbit anti-Actin recombinant monoclonalantibody [BLR057F].
Simple Western: BRD9 Antibody [NBP3-14730] -
Simple Western: BRD9 Antibody [NBP3-14730] - Detection of human BRD9 by Simple Western. Samples: Whole cell lysate (0.4 mg/mL) from Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BRD9 recombinant monoclonal antibody [BLR153J] (NBP3-14730) used at 1:10, 1:50, 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram. Image internally validated.
Simple Western: BRD9 Antibody [NBP3-14730] -
Simple Western: BRD9 Antibody [NBP3-14730] - Detection of mouse BRD9 by Simple Western. Samples: Whole cell lysate (2.0 mg/mL) from CT26 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BRD9 recombinant monoclonal antibody [BLR153J] (NBP3-14730) used at 1:50 and 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram. Image internally validated.![Immunocytochemistry/ Immunofluorescence: BRD9 Antibody (BLR153J) [NBP3-14730]](https://resources.rndsystems.com/images/products/BRD9-Antibody-BLR153J-Immunocytochemistry-Immunofluorescence-NBP3-14730-img0002.jpg "Immunocytochemistry/ Immunofluorescence: BRD9 Antibody (BLR153J) [NBP3-14730]")
Immunocytochemistry/ Immunofluorescence: BRD9 Antibody (BLR153J) [NBP3-14730]
Immunocytochemistry/Immunofluorescence: BRD9 Antibody (BLR153J) [NBP3-14730] - Section of HDLM-2 cells. Antibody: Rabbit anti-BRD9 recombinant monoclonal antibody [BLR153J]. (Secondary: HRPconjugated goat anti-rabbit IgG.![Immunohistochemistry-Paraffin: BRD9 Antibody (BLR153J) [NBP3-14730]](https://resources.rndsystems.com/images/products/BRD9-Antibody-BLR153J-Immunohistochemistry-Paraffin-NBP3-14730-img0004.jpg "Immunohistochemistry-Paraffin: BRD9 Antibody (BLR153J) [NBP3-14730]")
Immunohistochemistry-Paraffin: BRD9 Antibody (BLR153J) [NBP3-14730]
Immunohistochemistry-Paraffin: BRD9 Antibody (BLR153J) [NBP3-14730] - Section of mouse plasmacytoma. Antibody: Rabbit anti-BRD9 recombinant monoclonal antibody [BLR153J]. Secondary: HRP-conjugatedgoat anti-rabbit IgG.![Western Blot: BRD9 Antibody (BLR153J) [NBP3-14730]](https://resources.rndsystems.com/images/products/BRD9-Antibody-BLR153J-Western-Blot-NBP3-14730-img0005.jpg "Western Blot: BRD9 Antibody (BLR153J) [NBP3-14730]")
Western Blot: BRD9 Antibody (BLR153J) [NBP3-14730]
Western Blot: BRD9 Antibody (BLR153J) [NBP3-14730] - Whole cell lysate (50 ug) prepared using NETN buffer fromJurkat cells (WT and KO). Antibody: Rabbit anti-BRD9recombinant monoclonal antibody [BLR153J] used at 1:1000. Secondary: HRP-conjugated goat antirabbit IgG. Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody [BLR057F].![Western Blot: BRD9 Antibody (BLR153J) [NBP3-14730]](https://resources.rndsystems.com/images/products/BRD9-Antibody-BLR153J-Western-Blot-NBP3-14730-img0006.jpg "Western Blot: BRD9 Antibody (BLR153J) [NBP3-14730]")
Western Blot: BRD9 Antibody (BLR153J) [NBP3-14730]
Western Blot: BRD9 Antibody (BLR153J) [NBP3-14730] - Whole cell lysate (30 ug) from Jurkat, HEK293T, K-562, MCF-7, and HeLa cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BRD9 recombinant monoclonal antibody [BLR153J] used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-COPB2 antibody.![Immunohistochemistry-Paraffin: BRD9 Antibody (BLR153J) [NBP3-14730]](https://resources.rndsystems.com/images/products/BRD9-Antibody-BLR153J-Immunohistochemistry-Paraffin-NBP3-14730-img0003.jpg "Immunohistochemistry-Paraffin: BRD9 Antibody (BLR153J) [NBP3-14730]")
Immunohistochemistry-Paraffin: BRD9 Antibody (BLR153J) [NBP3-14730]
Immunohistochemistry-Paraffin: BRD9 Antibody (BLR153J) [NBP3-14730] - Section of lung carcinoma. Antibody: Rabbit anti-BRD9 recombinant monoclonal antibody [BLR153J]. Secondary: HRP-conjugated goat antirabbit IgG.![Immunoprecipitation: BRD9 Antibody (BLR153J) [NBP3-14730]](https://resources.rndsystems.com/images/products/BRD9-Antibody-Immunoprecipitation-NBP3-14730-img0001.jpg "Immunoprecipitation: BRD9 Antibody (BLR153J) [NBP3-14730]")
Immunoprecipitation: BRD9 Antibody (BLR153J) [NBP3-14730]
Immunoprecipitation: BRD9 Antibody [NBP3-14730] - Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from HeLa cells preparedusing NETN lysis buffer. Antibodies: Rabbit anti-BRD9recombinant monoclonal antibody [BLR153J] (A700-153 lot2) used for IP at 20 ul/mg lysate. BRD9 was alsoimmunoprecipitated by a previous lot of this antibodyand a second antibody against a differentepitope of BRD9. For blottingimmunoprecipitated BRD9, was used at 1:1000.Chemiluminescence with an exposure time of 30 seconds.
BRD9 Antibody [NBP3-14730] -
Antibody: Rabbit anti-BRD9 recombinantmonoclonal antibody [BLR153J] or isotypecontrol (unshaded). Secondary: DyLight® 650-conjugatedgoat anti-rabbit IgG.Applications for BRD9 Antibody (BLR153J)
Application
Recommended Usage
Flow Cytometry
Fixed in 4% formaldehyde and permeabilized with 90% methanol. 0.5
Immunocytochemistry/ Immunofluorescence
1:100 - 1:500
Immunohistochemistry
1:100 - 1:500
Immunohistochemistry-Paraffin
1:100 - 1:500
Immunoprecipitation
6 ul/1 mg lysate
Simple Western
1:10-1:250
Western Blot
1:1000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections See Simple Western Antibody Database for Simple Western validation: Tested in Jurkat [0.4 mg/mL], separated by Size, antibody dilution of 1:10, 1:50, 1:250, apparent MW was 90-92 kDa
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
>95%
Formulation
Borate Buffered Saline (BBS) pH 8.2 with, BSA Free
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 2 - 8 C / 1 year from date of receipt
Background: BRD9
Long Name
Bromodomain Containing 9
Alternate Names
FLJ13441, LAVS3040, PRO9856
Gene Symbol
BRD9
Additional BRD9 Products
Product Documents for BRD9 Antibody (BLR153J)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for BRD9 Antibody (BLR153J)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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