BRM Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-90015

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence, Simple Western

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all BRM Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SPVQLHQLRAQILAYKMLARGQPLPETLQLAVQGKRTLPGLQQQQQQQQQQQQQQQQQQQQQQQPQQQPPQPQTQQQQQPALVNYNRPSGPGPELSGPSTPQKLPVP

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (82%), Rat (81%). Human reactivity reported in scientific literature (PMID: 26564006).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BRM Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: BRM Antibody [NBP1-90015]

Immunocytochemistry/ Immunofluorescence: BRM Antibody [NBP1-90015]

Immunocytochemistry/Immunofluorescence: BRM Antibody [NBP1-90015] - Staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: BRM Antibody [NBP1-90015]

Immunohistochemistry-Paraffin: BRM Antibody [NBP1-90015]

Immunohistochemistry-Paraffin: BRM Antibody [NBP1-90015] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: BRM Antibody [NBP1-90015]

Immunohistochemistry-Paraffin: BRM Antibody [NBP1-90015]

Immunohistochemistry-Paraffin: BRM Antibody [NBP1-90015] - Staining of human cerebral cortex shows moderate to strong nuclear positivity in neuronal cells.
Immunohistochemistry-Paraffin: BRM Antibody [NBP1-90015]

Immunohistochemistry-Paraffin: BRM Antibody [NBP1-90015]

Immunohistochemistry-Paraffin: BRM Antibody [NBP1-90015] - Staining of human fallopian tube shows strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: BRM Antibody [NBP1-90015]

Immunohistochemistry-Paraffin: BRM Antibody [NBP1-90015]

Immunohistochemistry-Paraffin: BRM Antibody [NBP1-90015] - Staining of human pancreas shows weak to moderate nuclear positivity in exocrine glandular cells.

Applications for BRM Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Simple Western

1:25
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BRM

BRM is encoded by this gene is a member of the SWI/SNF family of proteins and is highly similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. Two transcript variants encoding different isoforms have been found for this gene, which contains a trinucleotide repeat (CAG) length polymorphism.

Long Name

Probable global transcription activator SNF2L2

Alternate Names

BAF190B, EC 3.6.4.-, hBRM, SMARCA2, SNF2-alpha, SNF2A, SNF2L2

Gene Symbol

SMARCA2

Additional BRM Products

Product Documents for BRM Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for BRM Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for BRM Antibody - BSA Free

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Protocols

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FAQs

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