BST2 Antibody - Azide and BSA Free
Novus Biologicals | Catalog # H00000684-B02P
![Western Blot: BST2 Antibody [H00000684-B02P]](https://resources.rndsystems.com/images/products/BST2-Antibody-Western-Blot-H00000684-B02P-img0009.jpg "Western Blot: BST2 Antibody [H00000684-B02P]")
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Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Proximity Ligation Assay
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Mouse IgG
Format
Azide and BSA Free
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Product Specifications
Immunogen
BST2 (NP_004326.1, 1 a.a. - 180 a.a.) full-length human protein. MASTSYDYCRVPMEDGDKRCKLLLGIGILVLLIIVILGVPLIIFTIKANSEACRDGLRAVMECRNVTHLLQQELTEAQKGFQDVEAQAATCNHTVMALMASLDAEKAQGQKKVEELEGEITTLNHKLQDASAEVERLRRENQVLSVRIADKKYYPSSQDSSSAAAPQLLIVLLGLSALLQ
Specificity
BST2 - bone marrow stromal cell antigen 2,
Clonality
Polyclonal
Host
Mouse
Isotype
IgG
Description
Quality control test: Antibody reactive against mammalian transfected lysate.
Scientific Data Images for BST2 Antibody - Azide and BSA Free
Western Blot: BST2 Antibody [H00000684-B02P]
Western Blot: BST2 Antibody [H00000684-B02P] - Analysis of BST2 expression in human spleen.![Immunocytochemistry/ Immunofluorescence: BST2 Antibody [H00000684-B02P]](https://resources.rndsystems.com/images/products/BST2-Antibody-Immunocytochemistry-Immunofluorescence-H00000684-B02P-img0006.jpg "Immunocytochemistry/ Immunofluorescence: BST2 Antibody [H00000684-B02P]")
Immunocytochemistry/ Immunofluorescence: BST2 Antibody [H00000684-B02P]
Immunocytochemistry/Immunofluorescence: BST2 Antibody [H00000684-B02P] - Analysis of purified antibody to BST2 on HeLa cell. (antibody concentration 10 ug/ml)![Immunohistochemistry-Paraffin: BST2 Antibody [H00000684-B02P]](https://resources.rndsystems.com/images/products/BST2-Antibody-Immunohistochemistry-Paraffin-H00000684-B02P-img0007.jpg "Immunohistochemistry-Paraffin: BST2 Antibody [H00000684-B02P]")
Immunohistochemistry-Paraffin: BST2 Antibody [H00000684-B02P]
Immunohistochemistry-Paraffin: BST2 Antibody [H00000684-B02P] - Analysis of purified antibody to BST2 on formalin-fixed paraffin-embedded human kidney. (antibody concentration 3 ug/ml)![Western Blot: BST2 Antibody [H00000684-B02P]](https://resources.rndsystems.com/images/products/BST2-Antibody-Western-Blot-H00000684-B02P-img0001.jpg "Western Blot: BST2 Antibody [H00000684-B02P]")
Western Blot: BST2 Antibody [H00000684-B02P]
Western Blot: BST2 Antibody [H00000684-B02P] - analysis of BST2 expression in transfected 293T cell line by BST2 MaxPab polyclonal antibody. Lane 1: BST2 transfected lysate (19.8 KDa). Lane 2: Non-transfected lysate.![Western Blot: BST2 Antibody [H00000684-B02P]](https://resources.rndsystems.com/images/products/BST2-Antibody-Western-Blot-H00000684-B02P-img0008.jpg "Western Blot: BST2 Antibody [H00000684-B02P]")
Western Blot: BST2 Antibody [H00000684-B02P]
Western Blot: BST2 Antibody [H00000684-B02P] - Analysis of BST2 expression in Jurkat.![Western Blot: BST2 Antibody [H00000684-B02P]](https://resources.rndsystems.com/images/products/BST2-Antibody-Western-Blot-H00000684-B02P-img0010.jpg "Western Blot: BST2 Antibody [H00000684-B02P]")
Western Blot: BST2 Antibody [H00000684-B02P]
Western Blot: BST2 Antibody [H00000684-B02P] - Analysis of BST2 expression in transfected 293T cell line by BST2 polyclonal antibody. Lane 1: BST2 transfected lysate(19.8 KDa). Lane 2: Non-transfected lysate.Applications for BST2 Antibody - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Proximity Ligation Assay
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against tissue lysate and transfected lysate in WB. May also be used for IF, IHC-P and ELISA. Knock Out Validation was reported in scientific literature (PMID: 27657169).
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
PBS (pH 7.4)
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: BST-2/Tetherin
Long Name
Bone Marrow Stromal Cell Antigen 2
Alternate Names
BST2, CD317, HM1.24 Antigen, NPC-A-7, Tetherin
Entrez Gene IDs
684 (Human)
Gene Symbol
BST2
UniProt
Additional BST-2/Tetherin Products
Product Documents for BST2 Antibody - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for BST2 Antibody - Azide and BSA Free
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for BST2 Antibody - Azide and BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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