BST2 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-45265

Novus Biologicals

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Primate

Predicted:

Chimpanzee (100%), Orangutan (100%), Rhesus Macaque (94%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all BST-2/Tetherin Primary Antibodies »

Product Specifications

Immunogen

A portion of amino acids 80-130 of human BST2 was used as the immunogen for this antibody.

Reactivity Notes

The BST2 amino acid sequence used as immunogen for this antibody is 100% homologous in White-cheeked Gibbon, Great Ape, and Gibbon.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit BST2 Antibody - Azide and BSA Free (NBP2-27154) is a polyclonal antibody validated for use in IHC, WB, Flow and Simple Western. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for BST2 Antibody - Azide and BSA Free

Western Blot: BST2 AntibodyAzide Free [NBP2-45265]

Western Blot: BST2 AntibodyAzide Free [NBP2-45265]

Western Blot: BST2 Antibody - Azide Free [NBP2-45265] - Analysis using BST2 antibody. Human heart in the 1) absence and 2) presence of immunizing peptide, 3) human Jurkat, 4) mouse RAW lysate and 5) recombinant human BST2 protein probed with BST2 antibody at 4 ug/mL. Goat anti-rabbit IgG HRP secondary antibody and PicoTect ECL substrate solution were used for this test. Image using the standard format of this product.
Immunohistochemistry: BST2 Antibody - Azide Free [NBP2-45265]

Immunohistochemistry: BST2 Antibody - Azide Free [NBP2-45265]

Immunohistochemistry: BST2 Antibody - Azide Free [NBP2-45265] - Analysis of BST2 in human liver tissue using BST2 antibody at 5 ug/mL. Image using the standard format of this product.
Immunohistochemistry: BST2 Antibody - Azide Free [NBP2-45265]

Immunohistochemistry: BST2 Antibody - Azide Free [NBP2-45265]

Immunohistochemistry: BST2 Antibody - Azide Free [NBP2-45265] - Mouse femur bone marrow section stained with BST2 antibody (red) and DAPI (blue). Image from a verified customer review. Image using the standard format of this product.
Immunohistochemistry: BST2 Antibody - Azide Free [NBP2-45265]

Immunohistochemistry: BST2 Antibody - Azide Free [NBP2-45265]

Immunohistochemistry: BST2 Antibody - Azide Free [NBP2-45265] - BST-2 (green) was detected in human skin (nevus) using Ang-2 antibody (1:50; 1 hour) and secondary anti-rabbit-FITC (1:500; 30 min) in PBS. Nuclei were stained with DAPI (blue). Tissue was fixed in acetone. Image from a verified customer review. Image using the standard format of this product.
Simple Western: BST2 AntibodyAzide Free [NBP2-45265]

Simple Western: BST2 AntibodyAzide Free [NBP2-45265]

Simple Western: BST2 Antibody - Azide Free [NBP2-45265] - Lane view shows a specific band for BST2 using 0.5 mg/mL Jurkat cell lysate and antibody at 1:200. Electoropherogram image of corresponding Simple Western lane view. Image reported by internal validation.
Simple Western: BST2 AntibodyAzide Free [NBP2-45265]

Simple Western: BST2 AntibodyAzide Free [NBP2-45265]

Simple Western: BST2 Antibody - Azide Free [NBP2-45265] - Simple Western lane view shows a specific band for BST2 in 0.5 mg/ml of Jurkat lysate. This experiment was performed under reducing A246 conditions using the 12-230 kDa separation system. Image using the standard format of this product.

Applications for BST2 Antibody - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

reported in scientific literature (Crother et al (2011); PMID 21695198)

Immunohistochemistry-Paraffin

5 ug/ml

Simple Western

1:200

Western Blot

2-4 ug/ml
Application Notes

BST2 is a 180 amino acid protein with an observed molecular weight of approximately 30 kDa in western blotting (see Ishikawa et all, 1995 PMID 7607676). In Simple Western only 10-15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: antibody dilution of 1:200

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: BST-2/Tetherin

BST2, also known as CD317, HM1.24 antigen, DAMP-2 and recently designated tetherin, is an integral membrane protein with a novel topology. The specific function of the BST2, encoded by the bone marrow stromal cell antigen 2 gene, is undetermined; however, it may play a role in pre-B-cell growth, rheumatoid arthritis, and in antiretroviral defense, that blocks release of retrovirus from the cell surface. BST2 is highly expressed during B-cell development, from pro-B precursors to plasma cells, T-cells, monocytes, Nk cells and DCs (at protein level).

Long Name

Bone Marrow Stromal Cell Antigen 2

Alternate Names

BST2, CD317, HM1.24 Antigen, NPC-A-7, Tetherin

Gene Symbol

BST2

Additional BST-2/Tetherin Products

Product Documents for BST2 Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for BST2 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for BST2 Antibody - Azide and BSA Free

There are currently no reviews for this product. Be the first to review BST2 Antibody - Azide and BSA Free and earn rewards!

Have you used BST2 Antibody - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies