BST2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-27154

Novus Biologicals

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Primate

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Flow (Cell Surface), Immunocytochemistry/ Immunofluorescence, Simple Western

Cited:

Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Flow (Cell Surface), Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all BST-2/Tetherin Primary Antibodies »

Product Specifications

Immunogen

A portion of amino acids 80-130 of human BST2 was used as the immunogen for this antibody.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit BST2 Antibody - BSA Free (NBP2-27154) is a polyclonal antibody validated for use in IHC, WB, Flow, ICC/IF and Simple Western. Anti-BST2 Antibody: Cited in 4 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for BST2 Antibody - BSA Free

Western Blot: BST2 Antibody [NBP2-27154]

Western Blot: BST2 Antibody [NBP2-27154]

Western Blot: BST2 Antibody [NBP2-27154] - Analysis using BST2 antibody. Human heart in the 1) absence and 2) presence of immunizing peptide, 3) human Jurkat, 4) mouse RAW lysate and 5) recombinant human BST2 protein probed with BST2 antibody at 4 ug/mL. Goat anti-rabbit IgG HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Immunohistochemistry-Frozen: BST2 Antibody [NBP2-27154]

Immunohistochemistry-Frozen: BST2 Antibody [NBP2-27154]

Immunohistochemistry-Frozen: BST2 Antibody [NBP2-27154] - BST-2 (green) was detected in human skin (nevus) using Ang-2 antibody (1:50; 1 hour) and secondary anti-rabbit-FITC (1:500; 30 min) in PBS. Nuclei were stained with DAPI (blue). Tissue was fixed in acetone. Image from a verified customer review.
Immunohistochemistry-Paraffin: BST2 Antibody [NBP2-27154]

Immunohistochemistry-Paraffin: BST2 Antibody [NBP2-27154]

Immunohistochemistry-Paraffin: BST2 Antibody [NBP2-27154] - Analysis of BST2 in human liver tissue using BST2 antibody at 5 ug/mL.
Simple Western: BST2 Antibody [NBP2-27154]

Simple Western: BST2 Antibody [NBP2-27154]

Simple Western: BST2 Antibody [NBP2-27154] - Simple Western lane view shows a specific band for BST2 in 0.5 mg/ml of Jurkat lysate. This experiment was performed under standard reducing conditions using the 12-230 kDa separation system.
Immunofluorescence: BST2 Antibody [NBP2-27154]

Immunofluorescence: BST2 Antibody [NBP2-27154]

Immunofluorescence: BST2 Antibody [NBP2-27154] - Mouse femur bone marrow section stained with BST2 antibody (red) and DAPI (blue). Image from a verified customer review.
BST2 Antibody

Simple Western: BST2 Antibody [NBP2-27154] -

Simple Western: BST2 Antibody [NBP2-27154] - Lane view shows a specific band for BST2 using 0.5 mg/mL Jurkat cell lysate and antibody at 1:200. Electoropherogram image of corresponding Simple Western lane view. Image reported by internal validation.

Applications for BST2 Antibody - BSA Free

Application
Recommended Usage

Flow (Cell Surface)

reported in scientific literature (PMID 21695198)

Immunohistochemistry-Frozen

reported by customer review

Immunohistochemistry-Paraffin

5 ug/mL

Simple Western

1:200

Western Blot

2 - 4 ug/mL
Application Notes

BST2 is a 180 amino acid protein with an observed molecular weight of approximately 30 kDa in Western blot (see Ishikawa et all, 1995 PMID 7607676).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in Jurkat lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:200, apparent MW was 21 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.

Reviewed Applications

Read 3 reviews rated 4 using NBP2-27154 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BST-2/Tetherin

BST2, also known as CD317, HM1.24 antigen, DAMP-2 and recently designated tetherin, is an integral membrane protein with a novel topology. The specific function of the BST2, encoded by the bone marrow stromal cell antigen 2 gene, is undetermined; however, it may play a role in pre-B-cell growth, rheumatoid arthritis, and in antiretroviral defense, that blocks release of retrovirus from the cell surface. BST2 is highly expressed during B-cell development, from pro-B precursors to plasma cells, T-cells, monocytes, Nk cells and DCs (at protein level).

Long Name

Bone Marrow Stromal Cell Antigen 2

Alternate Names

BST2, CD317, HM1.24 Antigen, NPC-A-7, Tetherin

Gene Symbol

BST2

UniProt

Q10589 (Human)

Additional BST-2/Tetherin Products

Product Documents for BST2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for BST2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for BST2 Antibody - BSA Free

Customer Reviews for BST2 Antibody - BSA Free (3)

4 out of 5
3 Customer Ratings
5 Stars
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4 Stars
100%
3 Stars
0%
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1 Stars
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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • BST2 Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: Frozen human skin sections
    Species: Human
    Verified Customer | Posted 07/18/2019
    BST-2 (green) was detected in human skin (nevus) using Ang-2 antibody (1:50; 1 hour) and secondary anti-rabbit-FITC (1:500; 30 min) in PBS. Nuclei were stained with Dapi (blue). Tissue was fixed in acetone.
    BST2 Antibody - BSA Free NBP2-27154
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested:
    Species: Human
    Verified Customer | Posted 01/04/2016
  • Name: James Fox
    Application: Immunofluorescence
    Sample Tested:
    Species: Mouse
    Verified Customer | Posted 08/06/2015
    Immunohistochemistry of mouse femur bone marrow section stained with BST2 antibody (NBP2-27154) [red] and DAPI (blue)
    BST2 Antibody - BSA Free NBP2-27154

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