BTF Antibody (M33-P5B11) - BSA Free
Novus Biologicals | Catalog # NBP2-50118
![Western Blot: BTF Antibody (M33-P5B11)BSA Free [NBP2-50118]](https://resources.rndsystems.com/images/products/BTF-Antibody-M33-P5B11-Western-Blot-NBP2-50118-img0005.jpg "Western Blot: BTF Antibody (M33-P5B11)BSA Free [NBP2-50118]")
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot, ELISA
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # M33-P5B11
Format
BSA Free
Loading...
Product Specifications
Immunogen
Peptide Sequence - KYQGDGIVED (amino acids 900 - 909)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for BTF Antibody (M33-P5B11) - BSA Free
Western Blot: BTF Antibody (M33-P5B11)BSA Free [NBP2-50118]
Western Blot: BTF Antibody (M33-P5B11) [NBP2-50118] - Western Blot using mAb M33-P5B11.![Immunohistochemistry: BTF Antibody (M33-P5B11) - BSA Free [NBP2-50118]](https://resources.rndsystems.com/images/products/BTF-Antibody-M33-P5B11-Immunohistochemistry-NBP2-50118-img0002.jpg "Immunohistochemistry: BTF Antibody (M33-P5B11) - BSA Free [NBP2-50118]")
Immunohistochemistry: BTF Antibody (M33-P5B11) - BSA Free [NBP2-50118]
Immunohistochemistry: BTF Antibody (M33-P5B11) [NBP2-50118] - IHC was performed on formalin-fixed, paraffin-embedded tissue sections using Anti-BCLAF1 [M33-P5B11]. Image is showing both cytoplasmic and nuclear localisation positivity of BCLAF1 in colon cancer which is in agreement with existing literature. Exclusively nuclear localisation of BCLAF1 is observed in non-neoplastic colon mucosa. Antigen retrieval step was required (microwave 10 min @ 950W in 0.01M sodium citrate buffer, pH 6.0).Applications for BTF Antibody (M33-P5B11) - BSA Free
Application
Recommended Usage
ELISA
1:100 - 1:2000
Immunohistochemistry
1:10 - 1:500
Western Blot
1:100 - 1:2000
Application Notes
Positive control(s): ELISA- Peptide immunogen; Western Blot- Hela whole cell extract; IHC- Human colon carcinoma.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: BTF
Alternate Names
bcl-2-associated transcription factor 1, BCL2-associated transcription factor 1, Btf, BTFKIAA0164bK211L9.1
Gene Symbol
BCLAF1
Additional BTF Products
Product Documents for BTF Antibody (M33-P5B11) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for BTF Antibody (M33-P5B11) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Customer Reviews for BTF Antibody (M33-P5B11) - BSA Free
There are currently no reviews for this product. Be the first to review BTF Antibody (M33-P5B11) - BSA Free and earn rewards!
Have you used BTF Antibody (M33-P5B11) - BSA Free?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...