Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # OTI2E4
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Product Specifications
Immunogen
Full length human recombinant protein of human BTLA(NP_861445) produced in HEK293T cell.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Theoretical MW
32.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for BTLA/CD272 Antibody (OTI2E4)
![Western Blot: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]](https://resources.rndsystems.com/images/products/BTLA-CD272-Antibody-2E4-Western-Blot-NBP2-45549-img0015.jpg "Western Blot: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]")
Western Blot: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]
Western Blot: BTLA/CD272 Antibody (2E4) [NBP2-45549] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTLA.![Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]](https://resources.rndsystems.com/images/products/BTLA-CD272-Antibody-2E4-Immunohistochemistry-NBP2-45549-img0014.jpg "Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]")
Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]
Immunohistochemistry: BTLA/CD272 Antibody (2E4) [NBP2-45549] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)![Flow Cytometry: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]](https://resources.rndsystems.com/images/products/BTLA-CD272-Antibody-2E4-Flow-Cytometry-NBP2-45549-img0001.jpg "Flow Cytometry: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]")
Flow Cytometry: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]
Flow Cytometry: BTLA/CD272 Antibody (2E4) [NBP2-45549] - Analysis of live Sup-B15 cells, using BTLA antibody, compared to IgG1 isotype control (Black).![Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]](https://resources.rndsystems.com/images/products/BTLA-CD272-Antibody-2E4-Immunohistochemistry-NBP2-45549-img0002.jpg "Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]")
Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]
Immunohistochemistry: BTLA/CD272 Antibody (2E4) [NBP2-45549] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)![Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]](https://resources.rndsystems.com/images/products/BTLA-CD272-Antibody-2E4-Immunohistochemistry-NBP2-45549-img0003.jpg "Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]")
Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]
Immunohistochemistry: BTLA/CD272 Antibody (2E4) [NBP2-45549] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)![Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]](https://resources.rndsystems.com/images/products/BTLA-CD272-Antibody-2E4-Immunohistochemistry-NBP2-45549-img0004.jpg "Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]")
Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]
Immunohistochemistry: BTLA/CD272 Antibody (2E4) [NBP2-45549] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)![Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]](https://resources.rndsystems.com/images/products/BTLA-CD272-Antibody-2E4-Immunohistochemistry-NBP2-45549-img0005.jpg "Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]")
Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]
Immunohistochemistry: BTLA/CD272 Antibody (2E4) [NBP2-45549] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)![Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]](https://resources.rndsystems.com/images/products/BTLA-CD272-Antibody-2E4-Immunohistochemistry-NBP2-45549-img0006.jpg "Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]")
Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]
Immunohistochemistry: BTLA/CD272 Antibody (2E4) [NBP2-45549] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)![Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]](https://resources.rndsystems.com/images/products/BTLA-CD272-Antibody-2E4-Immunohistochemistry-NBP2-45549-img0007.jpg "Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]")
Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]
Immunohistochemistry: BTLA/CD272 Antibody (2E4) [NBP2-45549] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)![Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]](https://resources.rndsystems.com/images/products/BTLA-CD272-Antibody-2E4-Immunohistochemistry-NBP2-45549-img0008.jpg "Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]")
Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]
Immunohistochemistry: BTLA/CD272 Antibody (2E4) [NBP2-45549] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)![Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]](https://resources.rndsystems.com/images/products/BTLA-CD272-Antibody-2E4-Immunohistochemistry-NBP2-45549-img0009.jpg "Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]")
Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]
Immunohistochemistry: BTLA/CD272 Antibody (2E4) [NBP2-45549] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)![Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]](https://resources.rndsystems.com/images/products/BTLA-CD272-Antibody-2E4-Immunohistochemistry-NBP2-45549-img0010.jpg "Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]")
Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]
Immunohistochemistry: BTLA/CD272 Antibody (2E4) [NBP2-45549] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)![Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]](https://resources.rndsystems.com/images/products/BTLA-CD272-Antibody-2E4-Immunohistochemistry-NBP2-45549-img0011.jpg "Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]")
Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]
Immunohistochemistry: BTLA/CD272 Antibody (2E4) [NBP2-45549] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)![Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]](https://resources.rndsystems.com/images/products/BTLA-CD272-Antibody-2E4-Immunohistochemistry-NBP2-45549-img0012.jpg "Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]")
Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]
Immunohistochemistry: BTLA/CD272 Antibody (2E4) [NBP2-45549] - Analysis of Carcinoma of Human lung tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)![Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]](https://resources.rndsystems.com/images/products/BTLA-CD272-Antibody-2E4-Immunohistochemistry-NBP2-45549-img0013.jpg "Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]")
Immunohistochemistry: BTLA/CD272 Antibody (OTI2E4) [NBP2-45549]
Immunohistochemistry: BTLA/CD272 Antibody (2E4) [NBP2-45549] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)Applications for BTLA/CD272 Antibody (OTI2E4)
Application
Recommended Usage
Flow Cytometry
1:100
Immunohistochemistry
1:150
Immunohistochemistry-Paraffin
1:150
Western Blot
1:4000
Reviewed Applications
Read 1 review rated 3 using NBP2-45549 in the following applications:
Flow Cytometry Panel Builder
Bio-Techne Knows Flow Cytometry
Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: BTLA
Long Name
B- And T-Lymphocyte Attenuator
Alternate Names
CD272
Gene Symbol
BTLA
Additional BTLA Products
Product Documents for BTLA/CD272 Antibody (OTI2E4)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for BTLA/CD272 Antibody (OTI2E4)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Customer Reviews for BTLA/CD272 Antibody (OTI2E4) (1)
3 out of 5
1 Customer Rating
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Application: ELISASample Tested: Human serumSpecies: HumanVerified Customer | Posted 06/21/2017
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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